Medium hydrated collagen gel as an explant support in organ culture

1982 ◽  
Vol 7 (3) ◽  
pp. 117-122 ◽  
Author(s):  
James A. McAteer ◽  
Thomas J. Cavanagh
Keyword(s):  
Organ Culture ◽  
Collagen Gel ◽  
Hydrated Collagen Gel

Characterization of materials ejected by excimer laser ablation of hydrated collagen gel

2002 ◽  
Vol 197-198 ◽  
pp. 782-785 ◽  
Author(s):  
Katsunori Tsunoda ◽  
Daisuke Kumaki ◽  
Teruo Takahashi ◽  
Hirofumi Yajima ◽  
Tadahiro Ishii ◽  
...  
Keyword(s):  
Laser Ablation ◽  
Excimer Laser ◽  
Collagen Gel ◽  
Excimer Laser Ablation ◽  
Characterization Of Materials ◽  
Hydrated Collagen Gel

scholarly journals Cellular events and behaviors after grafting of stratified squamous epithelial cell sheet onto a hydrated collagen gel

FEBS Open Bio ◽  
2017 ◽  
Vol 7 (5) ◽  
pp. 691-704 ◽  
Author(s):  
Yoshiyuki Kasai ◽  
Naoya Takeda ◽  
Shinichiro Kobayashi ◽  
Ryo Takagi ◽  
Masayuki Yamato
Keyword(s):  
Epithelial Cell ◽  
Cell Sheet ◽  
Collagen Gel ◽  
Squamous Epithelial Cell ◽  
Cellular Events ◽  
And Behaviors ◽  
Hydrated Collagen Gel

Culture of Human Laryngeal Papilloma Cells in Vitro

1982 ◽  
Vol 90 (6) ◽  
pp. 728-735 ◽  
Author(s):  
Bettie M. Steinberg ◽  
Allan L. Abramson ◽  
Raymond P. Meade
Keyword(s):  
Tissue Culture ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Collagen Gel ◽  
Primary Cells ◽  
Normal Epithelium ◽  
Normal Cells ◽  
Laryngeal Papilloma ◽  
Hydrated Collagen Gel

Human laryngeal epithelial cells have been grown in tissue culture in a hydrated collagen gel containing Nutrient Mixture F12 (Gibco) supplemented with 15% fetal calf serum and 10μg//mL hydrocortisone. Primary cells often remain viable in culture for more than six months. They can be serially transferred two to four times before senescence. Cells derived both from normal epithelium and from laryngeal papilloma have been successfully cultured. Papilloma cells appear to contain more perinuclear granules and form fewer tight junctions than normal cells.

scholarly journals Establishment and transformation diminish the ability of fibroblasts to contract a native collagen gel.

1980 ◽  
Vol 87 (1) ◽  
pp. 304-308 ◽  
Author(s):  
B M Steinberg ◽  
K Smith ◽  
M Colozzo ◽  
R Pollack
Keyword(s):  
Serum Concentration ◽  
Human Fibroblasts ◽  
Collagen Gel ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Collagen Contraction ◽  
Absolute Efficiency ◽  
Sv40 Transformation ◽  
Native Collagen ◽  
Hydrated Collagen Gel

Cultures of established and transformed fibroblasts were less able to contract a hydrated collagen gel than normal precrisis cells. Postcrisis fibroblasts from different rodent strains and species underwent a further reduction in contraction ability and either spontaneous or simian virus 40 (SV40) transformation. Human precrisis fibroblasts contracted much more efficiently than two SV40-transformed human lines. Fibroblasts from a patient with Glanzmann's thrombasthenia were intermediate between all other human fibroblasts assayed and the SV40-transformed human lines. The absolute efficiency of contraction was dependent on temperature and serum concentration, but no conditions were found that resulted in equal efficiencies for the three types of cells. Precrisis cells were extremely sensitive to the passage procedures when assayed for collagen contraction.

Delivery of Transforming Growth Factor-β2-Perturbing Antibody in a Collagen Vehicle Inhibits Cranial Suture Fusion in Calvarial Organ Culture

2003 ◽  
Vol 40 (3) ◽  
pp. 225-232 ◽  
Author(s):  
Amr M. Moursi ◽  
Phillip L. Winnard ◽  
Doug Fryer ◽  
Mark P. Mooney
Keyword(s):  
Growth Factor ◽  
Organ Culture ◽  
Fibroblast Growth Factor ◽  
Collagen Gel ◽  
Fibroblast Growth Factor 2 ◽  
Blue Staining ◽  
Fibroblast Growth ◽  
Cranial Suture ◽  
Antibody Treatment ◽  
Suture Fusion

Objective To determine whether antibody perturbation of Tgf-β, delivered in a collagen gel, could inhibit cranial suture fusion. Design Attachment and proliferation of osteoblasts cultured on a collagen gel with or without anti-Tgf-β2 antibody were determined by AlamarBlue dye assay and cell morphology by toluidine-blue staining. In rat calvarial organ culture, collagen gel with and without anti-Tgf-β2 antibody was injected subperiosteally over the posterior frontal suture of postnatal day 15 rat calvariae. A quantitative analysis of suture fusion was used to measure suture bridging in histological serial sections at various time points. Results Attachment and proliferation for cells cultured on collagen gel with anti-Tgf-β2 antibody were similar to collagen gel controls. Although proliferation was lower than on tissue culture plastic, cells treated with anti-Tgf-β2 antibody maintained an osteoblastic morphology. After 7, 10, and 15 days in organ culture, anti-Tgf-β2 antibody treatment caused a reduction in the percent bridging of posterior frontal sutures, compared with controls. Sutures exposed to anti-Tgf-β2 antibody and fibroblast growth factor-2 concurrently did not show an inhibition of bony bridging. Conclusions These results support previous reports suggesting a role for Tgf-β2 in cranial suture fusion. In cell culture the collagen gel, both with and without anti-Tgf-β2 antibody, promoted similar osteoblast attachment, proliferation, and osteoblastic morphology. In organ culture anti-Tgf-β2 antibody was delivered in a bioactive state via a collagen gel to inhibit cranial suture fusion. Also, the results suggest that the inductive effect of fibroblast growth factor-2 is not dependent on Tgf-β2 activity. Together, these results provide further support for the role of Tgf-β2 in cranial suture fusion.

Gamma-Irradiation Markedly Inhibits the Hydrated Collagen Gel Contraction by Arterial Smooth Muscle Cells

2001 ◽  
Vol 49 (3) ◽  
pp. 258-264 ◽  
Author(s):  
Pierre-Frederic Keller ◽  
Vitali Verin ◽  
Thierry Ziegler ◽  
Bernadette Mermillod ◽  
Youri Popowski ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Gamma Irradiation ◽  
Collagen Gel ◽  
Muscle Cells ◽  
Arterial Smooth Muscle ◽  
Collagen Gel Contraction ◽  
Arterial Smooth Muscle Cells ◽  
Hydrated Collagen Gel
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