Preparation of Chinese hamster bone marrow and spleen primary cell cultures for sister chromatid exchange and chromosomal aberration studies

1986 ◽  
Vol 9 (4) ◽  
pp. 199-203 ◽  
Author(s):  
G. Krishna ◽  
J. Nath ◽  
T. Ong
Keyword(s):  
Bone Marrow ◽  
Chromosomal Aberration ◽  
Cell Cultures ◽  
Sister Chromatid ◽  
Sister Chromatid Exchange ◽  
Chinese Hamster ◽  
Primary Cell ◽  
Primary Cell Cultures

scholarly journals Importance of the Toxicological Tests in the Application and Safety of Ozone Therapy

2018 ◽  
Vol 2 (3) ◽  
Author(s):  
Silvia Menendez-Cepero ◽  
Zullyt Zamora ◽  
Frank Hernandez
Keyword(s):  
Bone Marrow ◽  
Chromosomal Aberration ◽  
Sister Chromatid ◽  
Sister Chromatid Exchange ◽  
Micronucleus Assay ◽  
Ozone Therapy ◽  
Toxicological Studies

Until today, there is not any official registry of the different routes of systemic ozone therapy. With the aim to prove the safety of this therapy, several ways of ozone (O3) applications were evaluated, such as rectal insufflation (RI), major autohemotherapy (M-AHT) and intraperitoneal application (IP). For RI, some toxicological tests were performed such as: acute, sub-chronic, mutagenic (bone marrow chromosomic aberrations and micronucleus), teratogenic  and irritation studies (at 40 mg/L and 10 mL during 15 days). For M-AHT, acute toxicological (in rats with 21, 47 and 64 mg/L and a volume of 2 mL with daily evaluation up to 15 days) and mutagenic (chromosomal aberration analysis, micronucleus assay and sister chromatid exchange test) studies were performed. For IP application, subchronic (O3 was administered during 15 days in mice at concentrations of 11, 29 and 35 mg/L and volumes of 80 mL/kg) and genotoxic (cytotoxic and clastogenic activity) studies were evaluated. All the toxicological studies performed for the O3 RI fulfilled the requirements established for an official registry (no deleterious effects were observed). However, more toxicological studies must be done with respect to M-AHT and IP O3 applications in order to prove the complete safety of these ways.

scholarly journals Author Correction: Novel methods to establish whole‑body primary cell cultures for the cnidarians Nematostella vectensis and Pocillopora damicornis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
James D. Nowotny ◽  
Michael T. Connelly ◽  
Nikki Traylor‑Knowles
Keyword(s):  
Cell Cultures ◽  
Primary Cell ◽  
Whole Body ◽  
Nematostella Vectensis ◽  
Pocillopora Damicornis ◽  
Primary Cell Cultures ◽  
Novel Methods

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Detection and quantification of farnesol-induced apoptosis in difficult primary cell cultures by TaqMan protein assay

APOPTOSIS ◽  
2013 ◽  
Vol 18 (4) ◽  
pp. 452-466 ◽  
Author(s):  
Christina Pfister ◽  
Heike Pfrommer ◽  
Marcos S. Tatagiba ◽  
Florian Roser
Keyword(s):  
Cell Cultures ◽  
Primary Cell ◽  
Protein Assay ◽  
Primary Cell Cultures ◽  
Induced Apoptosis ◽  
Detection And Quantification
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