ABO blood grouping of bloodstains by sandwich ELISA using monoclonal antibody specific for human red cell band 3

1993 ◽  
Vol 105 (4) ◽  
pp. 209-212 ◽  
Author(s):  
A. Kimura ◽  
T. Uda ◽  
S. Nakashima ◽  
H. Ikeda ◽  
S. Yasuda ◽  
...  
Author(s):  
Arumugam P. ◽  
Swathandran Hamsavardhini ◽  
Ravishankar J.

Background: ABO discrepancies occur whenever the results of red cell grouping and serum grouping are in disagreement. The reasons for discrepancies both clinical and technical have to be sorted out. Further analysis is essential to resolve such discrepancies. If discrepancies are encountered, the interpretation of the ABO grouping has to be delayed until the same has been resolved. The aim of the study was to resolve ABO discrepancies encountered, by serological work up.Methods: All cases of discrepant samples received between August 2014 and May 2016 at the Department of Transfusion Medicine, The Tamilnadu Dr. MGR Medical University, Chennai, India were analyzed to determine the etiology by serological workup.Results: A total of twenty-one samples were analyzed and resolved. Fifteen cases of Type IV discrepancy, two cases of Type II discrepancy, one case Type III discrepancy, one case Type I discrepancy and two cases of technical errors were identified.Conclusions: ABO discrepancies can be resolved serologically if properly worked up. As ABO blood grouping is indispensible in blood transfusion service, it is imperative to resolve such discrepancies before transfusion.


1999 ◽  
Vol 4 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Naoyuki Araki ◽  
Naoto Koizumi ◽  
Fumie Kaneko ◽  
Nozomu Horie ◽  
Takako Nishino ◽  
...  

2018 ◽  
Vol 56 (1) ◽  
pp. 17
Author(s):  
Nguyen Thi Trung ◽  
Truong Nam Hai

So, it needs to balance the ratio of anti-A monoclonal antibody and anti-B monoclonal antibody  in the mixing so that the possibility of agglutination is the best. In this paper, anti-A monoclonal antibody (titer is 1/256) and anti-B monoclonal antibody (titer is 1/256) was used. The best results were obtained at one volume anti A monoclonal antibody is mixed one volume anti-B monoclonal antibody. The anti-A,B antibody titer was 1/128 for red blood group A and it was 1/128 for red blood group B. The intensity of agglutination reached  3+ for both red blood group A and B.


1991 ◽  
Vol 104 (5) ◽  
pp. 255-258 ◽  
Author(s):  
Akihiko Kimura ◽  
Fuseo Matsumura ◽  
Kenichiro Sodesaki ◽  
Motoki Osawal ◽  
Haruhiko Ikeda ◽  
...  

2006 ◽  
Vol 82 (6) ◽  
pp. 1595
Author(s):  
Stephen J. Wagner ◽  
Andrey Skripchenko ◽  
Dedeene Thompson-Montgomery ◽  
Helen Awatefe ◽  
David J. Donnelly ◽  
...  

2003 ◽  
Vol 7 (2) ◽  
pp. 145-153
Author(s):  
Yasunari Hisashi ◽  
Tatsuo Shinozuka ◽  
Osamu Ohue ◽  
Setsuko Takei ◽  
Ayako Ro ◽  
...  

Blood ◽  
2000 ◽  
Vol 96 (3) ◽  
pp. 1184-1186 ◽  
Author(s):  
Vivek R. Sharma ◽  
Donald R. Fleming ◽  
Stephen P. Slone

Abstract Rituximab is a chimeric monoclonal antibody directed against CD20 and used in the treatment of B-cell non-Hodgkin's lymphoma. Due to its ability to deplete B lymphocytes, rituximab can interfere with humoral immunity, causing it to be suppressed for several months after treatment. The reported case depicts a serious consequence of this effect of rituximab therapy: pure red cell aplasia resulting from chronic parvovirus B19 infection. The point of interest in this case is not only the association between rituximab therapy and pure red cell aplasia, but the diagnostic and therapeutic utility of the knowledge of parvovirus B19 as the likely etiologic link between the two. Given the known efficacy of intravenous immunoglobulin (IVIg) in the treatment of chronic parvovirus B19 infection, this therapy can cure some of these patients and successfully render most others transfusion-independent until recovery of their own humoral immune system.


2016 ◽  
Vol 1858 (7) ◽  
pp. 1507-1532 ◽  
Author(s):  
Reinhart A.F. Reithmeier ◽  
Joseph R. Casey ◽  
Antreas C. Kalli ◽  
Mark S.P. Sansom ◽  
Yilmaz Alguel ◽  
...  

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