Increased response of the rainbow trout gonad cell unscheduled DNA repair assay

1985 ◽  
Vol 34 (1) ◽  
pp. 340-348 ◽  
Author(s):  
Douglas G. Walton ◽  
Alfred B. Acton ◽  
Hans F. Stich
Keyword(s):  
Dna Repair ◽  
Rainbow Trout ◽  
Gonad Cell

scholarly journals Environmentally Relevant Mixture of Pesticides Affect Mobility and DNA Integrity of Early Life Stages of Rainbow Trout (Oncorhynchus mykiss)

Toxics ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 174
Author(s):  
Shannon Weeks Santos ◽  
Jérôme Cachot ◽  
Bettie Cormier ◽  
Nicolas Mazzella ◽  
Pierre-Yves Gourves ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Repair ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Protein Carbonyl ◽  
Swimming Activity ◽  
Mitochondrial Metabolism ◽  
Pesticide Mixture ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Mixture Concentration

The aim of this study was to analyze the impact of three concentrations of a pesticide mixture on the first development stages of rainbow trout (Oncorhynchus mykiss). The mixture was made up of three commonly used pesticides in viticulture: glyphosate (GLY), chlorpyrifos (CPF) and copper sulfate (Cu). Eyed stage embryos were exposed for 3 weeks to three concentrations of the pesticide mixture. Lethal and sub-lethal effects were assessed through a number of phenotypic and molecular endpoints including survival, hatching delay, hatching success, biometry, swimming activity, DNA damage (Comet assay), lipid peroxidation (TBARS), protein carbonyl content and gene expression. Ten target genes involved in antioxidant defenses, DNA repair, mitochondrial metabolism and apoptosis were analyzed using real-time RT-qPCR. No significant increase of mortality, half-hatch, growth defects, TBARS and protein carbonyl contents were observed whatever the pesticide mixture concentration. In contrast, DNA damage and swimming activity were significantly more elevated at the highest pesticide mixture concentration. Gene transcription was up-regulated for genes involved in detoxification (gst and mt1), DNA repair (ogg1), mitochondrial metabolism (cox1 and 12S), and cholinergic system (ache). This study highlighted the induction of adaptive molecular and behavioral responses of rainbow trout larvae when exposed to environmentally realistic concentrations of a mixture of pesticides.

DNA repair synthesis in isolated rainbow trout liver cells

1989 ◽  
Vol 10 (6) ◽  
pp. 995-1001 ◽  
Author(s):  
Michael R. Miller ◽  
James B. Blair ◽  
David E. Hinton
Keyword(s):  
Dna Repair ◽  
Rainbow Trout ◽  
Liver Cells ◽  
Repair Synthesis ◽  
Dna Repair Synthesis ◽  
Rainbow Trout Liver

Bacillus subtilis GSY 1057 Assay for Aflatoxin B1 Activation by Rainbow Trout (Salmo gairdneri)

1975 ◽  
Vol 58 (5) ◽  
pp. 1074-1076
Author(s):  
Grant L Schoenhard ◽  
Paul E Bishop ◽  
Donald J Lee ◽  
Russell O Sinnhuber
Keyword(s):  
Dna Repair ◽  
Bacillus Subtilis ◽  
Rainbow Trout ◽  
Aflatoxin B1 ◽  
Incubation Mixture ◽  
Salmo Gairdneri ◽  
Microbial Assay ◽  
Liver Homogenates ◽  
Base Plates ◽  
Blood Agar Base

Abstract A rapid and sensitive microbial assay was developed to detect lethal products of anatoxin B1 metabolism by rainbow trout (Salmon gairdneri) Mt. Shasta strain. Bacillus subtilis GSY 1057 (hisA1, uvr-1, metB4), a DNA repair deficient strain, was incubated for 20 min in the 20,000 × g supernate from trout liver homogenates which had been preincubated for 10 min with various levels of aflatoxin B1. Serial dilutions of the incubation mixture were plated in triplicate on tryptose blood agar base plates and colonies were counted after 12 hr at 37°C. One μmole aflatoxin B1 in 3.2 ml incubation mixture reduced viability 60°.

259: Dna-Repair Genetic Polymorphisms and Prostate Cancer Risk

2005 ◽  
Vol 173 (4S) ◽  
pp. 71-71
Author(s):  
Peter E. Clark ◽  
M. Craig Hall ◽  
Kristin L. Lockett ◽  
Jianfeng Xu ◽  
Sigun L. Zheng ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Dna Repair ◽  
Cancer Risk ◽  
Genetic Polymorphisms ◽  
Prostate Cancer Risk

983: Bladder Cancer Predisposition: A Pathway-Based Approach to DNA Repair and Cell Cycle Control Genes

2006 ◽  
Vol 175 (4S) ◽  
pp. 317-317
Author(s):  
Xifeng Wu ◽  
Jian Gu ◽  
H. Barton Grossman ◽  
Christopher I. Amos ◽  
Carol Etzel ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Bladder Cancer ◽  
Dna Repair ◽  
Cell Cycle Control ◽  
Cancer Predisposition

Drugs Impair Keratinocyte DNA Repair in Culture

2005 ◽  
Vol 36 (7) ◽  
pp. 42
Author(s):  
PATRICE WENDLING
Keyword(s):  
Dna Repair
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