Cloning of rat liver arginase cDNA and elucidation of regulation of arginase gene expression in H4 rat hepatoma cells

1986 ◽  
Vol 12 (4) ◽  
pp. 375-384 ◽  
Author(s):  
George J. Dizikes ◽  
Elaine B. Spector ◽  
Stephen D. Cederbaum
Keyword(s):  
Gene Expression ◽  
Rat Liver ◽  
Hepatoma Cells ◽  
Rat Hepatoma ◽  
Rat Hepatoma Cells

The characterization of mitochondrial translation products in rat liver and rat hepatoma cells

FEBS Letters ◽  
1981 ◽  
Vol 126 (1) ◽  
pp. 61-65 ◽  
Author(s):  
Jordan Kolarov ◽  
Štefan Kužela ◽  
Antek Wielburski ◽  
B.Dean Nelson
Keyword(s):  
Rat Liver ◽  
Hepatoma Cells ◽  
Mitochondrial Translation ◽  
Rat Hepatoma ◽  
Rat Hepatoma Cells

Osmotic Regulation of MAP-Kinase Activities and Gene Expression in H4IIE Rat Hepatoma Cells

1998 ◽  
Vol 379 (6) ◽  
pp. 667-672 ◽  
Author(s):  
Sabine Wiese ◽  
Freimut Schliess ◽  
Dieter Häussinger
Keyword(s):  
Gene Expression ◽  
Map Kinase ◽  
Hepatoma Cells ◽  
Osmotic Regulation ◽  
Rat Hepatoma ◽  
Rat Hepatoma Cells

Osmotic regulation of betaine homocysteine-S-methyltransferase expression in H4IIE rat hepatoma cells

2007 ◽  
Vol 292 (4) ◽  
pp. G1089-G1098 ◽  
Author(s):  
Christine Schäfer ◽  
Lars Hoffmann ◽  
Katrin Heldt ◽  
Mohammad Reza Lornejad-Schäfer ◽  
Gernot Brauers ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mrna Expression ◽  
Tyrosine Kinases ◽  
Hepatoma Cells ◽  
Pcr Analysis ◽  
Osmotic Regulation ◽  
A Cell ◽  
Gene Expression Studies ◽  
Rat Hepatoma ◽  
Rat Hepatoma Cells

Cell hydration changes critically affect liver metabolism and gene expression. In the course of gene expression studies using nylon cDNA-arrays we found that hyperosmolarity (405 mosmol/l) suppressed the betaine-homocysteine methyltransferase ( Bhmt) mRNA expression in H4IIE rat hepatoma cells. This was confirmed by Northern blot and real-time quantitative RT-PCR analysis, which in addition unraveled a pronounced induction of Bhmt mRNA expression by hypoosmotic (205 mosmol/l) swelling. Osmotic regulation of Bhmt mRNA expression was largely paralleled at the levels of Bhmt protein and enzymatic activity. Like hyperosmotic NaCl, hyperosmotic raffinose but not hyperosmotic urea suppressed Bhmt mRNA expression, suggesting that cell shrinkage rather than increased ionic strength or hyperosmolarity per se is the trigger. Hypoosmolarity increased the expression of a reporter gene driven by the entire human BHMT promoter, whereas destabilization of BHMT mRNA was observed under hyperosmotic conditions. Osmosensitivity of Bhmt mRNA expression was impaired by inhibitors of tyrosine kinases and cyclic nucleotide-dependent kinases. The osmotic regulation of BHMT may be part of a cell volume-regulatory response and additionally lead to metabolic alterations that depend on the availability of betaine-derived methyl groups.

scholarly journals Osmoregulated taurine transport in H4IIE hepatoma cells and perfused rat liver

1997 ◽  
Vol 321 (3) ◽  
pp. 683-690 ◽  
Author(s):  
Ulrich WARSKULAT ◽  
Matthias WETTSTEIN ◽  
Dieter HÄUSSINGER
Keyword(s):  
Rat Liver ◽  
Hepatoma Cells ◽  
Cell Swelling ◽  
Mrna Levels ◽  
Taurine Transport ◽  
H4iie Cells ◽  
Taurine Uptake ◽  
Rat Hepatoma ◽  
Taurine Efflux ◽  
Rat Hepatoma Cells

The effects of aniso-osmotic exposure on taurine transport were studied in H4IIE rat hepatoma cells. Hyperosmotic (405 mosmol/l) exposure of H4IIE cells stimulated Na+-dependent taurine uptake and led to an increase in taurine transporter (TAUT) mRNA levels, whereas hypo-osmotic (205 mosmol/l) exposure diminished both taurine uptake and TAUT mRNA levels when compared with normo-osmotic (305 mosmol/l) control incubations. Taurine uptake increased 30Ő40-fold upon raising the ambient osmolarity from 205 to 405 mosmol/l. When H4IIE cells and perfused livers were preloaded with taurine, hypo-osmotic cell swelling led to a rapid release of taurine from the cells. The taurine efflux, but not taurine uptake, was sensitive to 4,4ƀ-di-isothiocyanatostilbene-2,2ƀ-disulphonic acid (DIDS), suggestive of an involvement of DIDS-sensitive channels in mediating volume-regulatory taurine efflux. Whereas in both H4IIE rat hepatoma cells and primary hepatocytes TAUT mRNA levels were strongly dependent upon ambient osmolarity, mRNAs for other osmolyte transporters, i.e. the betaine transporter BGT-1 and the Na+/myo-inositol transporter SMIT, were not detectable. In line with this, myo-inositol uptake by H4IIE hepatoma cells was low and was not stimulated by hyperosmolarity. However, despite the absence of BGT-1 mRNA, a slight osmosensitive uptake of betaine was observed, but the rate was less than 10% of that of taurine transport. This study identifies a constitutively expressed and osmosensitive TAUT in H4IIE cells and the use of taurine as a main osmolyte, whereas betaine and myo-inositol play little or no role in the osmolyte strategy in these cells. This is in contrast with rat liver macrophages, in which betaine has been shown to be a major osmolyte.

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