Enzyme-linked immunosorbent assay for the detection of corn stunt spiroplasma in plant and insect tissues

1981 ◽  
Vol 5 (2) ◽  
pp. 101-104 ◽  
Author(s):  
Boligala C. Raju ◽  
George Nyland
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Corn Stunt ◽  
Corn Stunt Spiroplasma

Monoclonal antibodies against corn stunt spiroplasma

1985 ◽  
Vol 31 (10) ◽  
pp. 900-904 ◽  
Author(s):  
C. P. Lin ◽  
T. A. Chen
Keyword(s):  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
Polyclonal Antibodies ◽  
Double Diffusion ◽  
The Other ◽  
Diffusion Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Corn Stunt ◽  
Splenic Cells ◽  
Corn Stunt Spiroplasma

Monoclonal antibodies against the corn stunt spiroplasma were produced by fusing NS-1/1-Ag4-1 murine myeloma cells with splenic cells from mice immunized with corn stunt spiroplasma (I-747). Isotypes of these monoclonal antibodies have been determined to be IgG1 or IgG2a subclass by the Ouchterlony double diffusion method using class and subclass specific antibodies for mouse IgG1, IgG2a, IgG2b, IgG3, and IgM. Antibody titers for both hybridoma culture supernatants and ascitic fluids were determined with indirect biotinylated enzyme-linked immunosorbent assay and they ranged from 800 to 3200, and 312 500 to 7 812 400, respectively. In serological specificity studies, with indirect enzyme-linked immunosorbent assay, 32 spiroplasma strains belonging to 19 different serogroups were tested using both corn stunt spiroplasma monoclonal antibodies and conventionally produced polyclonal antibodies. Corn stunt spiroplasma specific monoclonal antibodies reacted specifically with any of the three corn stunt spiroplasma strains tested, but not with all of the other spiroplasma strains from different groups and subgroups. On the other hand, some nonspecific reactions to heterologous spiroplasma strains were obtained when conventional polyclonal antibodies were used.

Plasminogen Interactions with Immobilized Fibrinogen

1989 ◽  
Vol 62 (04) ◽  
pp. 1078-1082 ◽  
Author(s):  
Burt Adelman ◽  
Patricia Ouynn
Keyword(s):  
Immunosorbent Assay ◽  
Aminocaproic Acid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Binding Regions ◽  
Dose Dependent Fashion ◽  
Epsilon Aminocaproic Acid ◽  
Dose Dependent

SummaryThis report describes the binding of plasminogen to fibrinogen adsorbed onto polystyrene wells. Binding was determined by enzyme linked immunosorbent assay. Both glu- and lys-plasminogen bound to immobilized fibrinogen in a dose-dependent fashion. However, more lys- than glu-plasminogen bound when equal concentrations of either were added to immobilized fibrinogen. Plasminogen binding was inhibited by epsilon aminocaproic acid indicating that binding was mediated via lysine-binding regions of plasminogen. Soluble fibrinogen added in excess of immobilized fibrinogen did not compete for plasminogen binding but fibrinogen fragments produced by plasmin digestion of fibrinogen did. Treatment of immobilized fibrinogen with thrombin caused a small but significant (p <0.01) increase in plasminogen binding. These studies demonstrate that immobilized fibrinogen binds both glu- and lys-plasminogen and that binding is mediated via lysine-binding regions. These interactions may facilitate plasminogen binding to fibrinogen adsorbed on to surfaces and to cells such as platelets which bind fibrinogen.

Alterations in Vascular Endothelial Cell-related Plasma Proteins In Thalassaemic Patients and their Correlation with Clinical Symptoms

1995 ◽  
Vol 74 (04) ◽  
pp. 1045-1049 ◽  
Author(s):  
P Butthep ◽  
A Bunyaratvej ◽  
Y Funahara ◽  
H Kitaguchi ◽  
S Fucharoen ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Immunosorbent Assay ◽  
Plasma Proteins ◽  
Clinical Symptoms ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial Cell ◽  
Enzyme Linked Immunosorbent Assay ◽  
Vascular Endothelial ◽  
Leg Ulcers

SummaryAn increased level of plasma thrombomodulin (TM) in α- and β- thalassaemia was demonstrated using an enzyme-linked immunosorbent assay (ELISA). Nonsplenectomized patients with β-thalassaemia/ haemoglobin E (BE) had higher levels of TM than splenectomized cases (BE-S). Patients with leg ulcers (BE-LU) were found to have the highest increase in TM level. Appearance of larger platelets in all types of thalassaemic blood was observed indicating an increase in the number of younger platelets. These data indicate that injury of vascular endothelial cells is present in thalassaemic patients.

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