B lymphocyte alloantigens controlled by theI region of the major histocompatibility complex in mice

1974 ◽  
Vol 1 (1) ◽  
pp. 68-81 ◽  
Author(s):  
Günter J. Hämmerling ◽  
Beverly D. Deak ◽  
Gisela Mauve ◽  
Ulrich Hämmerling ◽  
Hugh O. McDevitt
Keyword(s):  
Major Histocompatibility Complex ◽  
B Lymphocyte ◽  
Major Histocompatibility ◽  
Histocompatibility Complex ◽  
Thei Region

scholarly journals Two distinct nuclear factors bind the conserved regulatory sequences of a rabbit major histocompatibility complex class II gene.

1988 ◽  
Vol 8 (5) ◽  
pp. 2034-2041 ◽  
Author(s):  
N Sittisombut
Keyword(s):  
Major Histocompatibility Complex ◽  
Cell Fusion ◽  
Mhc Class Ii ◽  
Dna Sequences ◽  
B Lymphocyte ◽  
Nuclear Extract ◽  
Class Ii ◽  
Regulatory Sequences ◽  
Major Histocompatibility ◽  
Histocompatibility Complex

The constitutive coexpression of the major histocompatibility complex (MHC) class II genes in B lymphocytes requires positive, trans-acting transcriptional factors. The need for these trans-acting factors has been suggested by the reversion of the MHC class II-negative phenotype of rare B-lymphocyte mutants through somatic cell fusion with B cells or T-cell lines. The mechanism by which the trans-acting factors exert their effect on gene transcription is unknown. The possibility that two highly conserved DNA sequences, located 90 to 100 base pairs (bp) (the A sequence) and 60 to 70 bp (the B sequence) upstream of the transcription start site of the class II genes, are recognized by the trans-acting factors was investigated in this study. By using the gel electrophoresis retardation assay, a minimum of two proteins which specifically bound the conserved A or B sequence of a rabbit DP beta gene were identified in murine nuclear extracts of a B-lymphoma cell line, A20-2J. Fractionation of nuclear extract through a heparin-agarose column allowed the identification of one protein, designated NF-MHCIIB, which bound an oligonucleotide containing the B sequence and protected the entire B sequence in the DNase I protection analysis. Another protein, designated NF-MHCIIA, which bound an oligonucleotide containing the A sequence and partially protected the 3' half of this sequence, was also identified. NF-MHCIIB did not protect a CCAAT sequence located 17 bp downstream of the B sequence. The possible relationship between these DNA-binding factors and the trans-acting factors identified in the cell fusion experiments is discussed.

scholarly journals Major histocompatibility complex-restricted antigen presentation to antigen-reactive T cells by B lymphocyte tumor cells.

1981 ◽  
Vol 154 (5) ◽  
pp. 1419-1431 ◽  
Author(s):  
D J McKean ◽  
A J Infante ◽  
A Nilson ◽  
M Kimoto ◽  
C G Fathman ◽  
...  
Keyword(s):  
Major Histocompatibility Complex ◽  
Antigen Presentation ◽  
Tumor Cells ◽  
Soluble Protein ◽  
B Lymphocyte ◽  
Antigen Presenting Cells ◽  
Major Histocompatibility ◽  
Protein Antigens ◽  
Histocompatibility Complex ◽  
Antigen Presenting

Previous reports have demonstrated that accessory cells function to present soluble protein antigens in association with gene products encoded within the I region of the major histocompatibility complex (MHC) to antigen-reactive T helper cells. The biochemical events that occur during antigen presentation are, however, not well-documented primarily because of the difficulties involved in purifying sufficient numbers of homogeneous antigen-presenting cells. In this paper, a number of Ia-positive B lymphocyte tumor lines are shown to be capable of presenting soluble protein antigens to antigen-reactive continuous T cell lines in an MHC-restricted fashion. The characterization of the antigen presentation function of these tumor cells indicates that the tumor cells have many of the functional antigen-presenting characteristics previously thought to be limited to macrophages. These tumor cells should provide a useful model system for determining the biochemical events that occur in antigen uptake and processing as well as for determining the potential interactions between processed antigen and Ia molecules on the plasma membrane of these antigen-presenting cells.

scholarly journals Anatomy of a new B-cell-specific enhancer.

1989 ◽  
Vol 9 (1) ◽  
pp. 303-311 ◽  
Author(s):  
W Koch ◽  
C Benoist ◽  
D Mathis
Keyword(s):  
Major Histocompatibility Complex ◽  
Major Histocompatibility Complex Class ◽  
B Cell ◽  
B Lymphocyte ◽  
Class Ii ◽  
Complex Class ◽  
Major Histocompatibility ◽  
Enhancer Activity ◽  
Histocompatibility Complex ◽  
Complex Anatomy

The major histocompatibility complex class II molecules, like the immunoglobulins, are prominent B-lymphocyte markers. Herein, we describe a B-cell-specific enhancer associated with the murine class II gene, Ek alpha. This enhancer has a complex anatomy that suggests interactions between remotely spaced elements. Of particular interest is the finding that two CCAAT boxes spaced one kilobase apart are important for enhancer activity. Somewhat surprisingly, the E alpha and immunoglobulin enhancers seem to show little resemblance.

Two distinct nuclear factors bind the conserved regulatory sequences of a rabbit major histocompatibility complex class II gene

1988 ◽  
Vol 8 (5) ◽  
pp. 2034-2041
Author(s):  
N Sittisombut
Keyword(s):  
Major Histocompatibility Complex ◽  
Cell Fusion ◽  
Mhc Class Ii ◽  
Dna Sequences ◽  
B Lymphocyte ◽  
Nuclear Extract ◽  
Class Ii ◽  
Regulatory Sequences ◽  
Major Histocompatibility ◽  
Histocompatibility Complex

The constitutive coexpression of the major histocompatibility complex (MHC) class II genes in B lymphocytes requires positive, trans-acting transcriptional factors. The need for these trans-acting factors has been suggested by the reversion of the MHC class II-negative phenotype of rare B-lymphocyte mutants through somatic cell fusion with B cells or T-cell lines. The mechanism by which the trans-acting factors exert their effect on gene transcription is unknown. The possibility that two highly conserved DNA sequences, located 90 to 100 base pairs (bp) (the A sequence) and 60 to 70 bp (the B sequence) upstream of the transcription start site of the class II genes, are recognized by the trans-acting factors was investigated in this study. By using the gel electrophoresis retardation assay, a minimum of two proteins which specifically bound the conserved A or B sequence of a rabbit DP beta gene were identified in murine nuclear extracts of a B-lymphoma cell line, A20-2J. Fractionation of nuclear extract through a heparin-agarose column allowed the identification of one protein, designated NF-MHCIIB, which bound an oligonucleotide containing the B sequence and protected the entire B sequence in the DNase I protection analysis. Another protein, designated NF-MHCIIA, which bound an oligonucleotide containing the A sequence and partially protected the 3' half of this sequence, was also identified. NF-MHCIIB did not protect a CCAAT sequence located 17 bp downstream of the B sequence. The possible relationship between these DNA-binding factors and the trans-acting factors identified in the cell fusion experiments is discussed.

Anatomy of a new B-cell-specific enhancer

1989 ◽  
Vol 9 (1) ◽  
pp. 303-311
Author(s):  
W Koch ◽  
C Benoist ◽  
D Mathis
Keyword(s):  
Major Histocompatibility Complex ◽  
Major Histocompatibility Complex Class ◽  
B Cell ◽  
B Lymphocyte ◽  
Class Ii ◽  
Complex Class ◽  
Major Histocompatibility ◽  
Enhancer Activity ◽  
Histocompatibility Complex ◽  
Complex Anatomy

The major histocompatibility complex class II molecules, like the immunoglobulins, are prominent B-lymphocyte markers. Herein, we describe a B-cell-specific enhancer associated with the murine class II gene, Ek alpha. This enhancer has a complex anatomy that suggests interactions between remotely spaced elements. Of particular interest is the finding that two CCAAT boxes spaced one kilobase apart are important for enhancer activity. Somewhat surprisingly, the E alpha and immunoglobulin enhancers seem to show little resemblance.

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