Genetic counterselective procedure to isolate interspecific cell hybrids containing single human chromosomes: Construction of cell hybrids and recombinant DNA libraries specific for human chromosomes 3 and 4

1986 ◽  
Vol 12 (2) ◽  
pp. 163-174 ◽  
Author(s):  
Leon R. Carlock ◽  
David Smith ◽  
John J. Wasmuth
Keyword(s):  
Recombinant Dna ◽  
Human Chromosomes ◽  
Cell Hybrids ◽  
Dna Libraries ◽  
Interspecific Cell Hybrids

scholarly journals A strategy to detect and isolate an intron-containing gene in the presence of multiple processed pseudogenes

1989 ◽  
Vol 86 (17) ◽  
pp. 6691-6695 ◽  
Author(s):  
B Davies ◽  
S Feo ◽  
E Heard ◽  
M Fried
Keyword(s):  
Ribosomal Protein ◽  
Recombinant Dna ◽  
Single Gene ◽  
Ribosomal Protein Gene ◽  
Pcr Primers ◽  
Pcr Product ◽  
Dna Libraries ◽  
Processed Pseudogenes ◽  
Polymerase Chain ◽  
Genomic Dna Sequence

We have devised a strategy that utilizes the polymerase chain reaction (PCR) for the detection and isolation of intron-containing genes in the presence of an abundance of processed pseudogenes. The method depends on the genomic DNA sequence between the PCR primers spanning at least one intron in the gene of interest, resulting in the generation of a larger intron-containing PCR product in addition to the smaller PCR product amplified from the intronless pseudogenes. A unique intron probe isolated from the larger PCR product is used for the detection of intron-containing clones from recombinant DNA libraries that also contain pseudogene clones. This method has been used successfully for the selective isolation of an intron-containing rat L19 ribosomal protein gene in the presence of multiple pseudogenes. Analysis of a number of mammalian ribosomal protein multigene families by PCR indicates that they all contain only a single gene with introns.

Localization of genes on human chromosomes by studies of human-Chinese hamster somatic cell hybrids

1973 ◽  
Vol 20 (3) ◽  
pp. 195-202 ◽  
Author(s):  
Ans Jongsma ◽  
Harry Someren ◽  
Andries Westerveld ◽  
Ann Hagemeijer ◽  
Peter Pearson
Keyword(s):  
Somatic Cell ◽  
Chinese Hamster ◽  
Human Chromosomes ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids

Mouse-Human Somatic Cell Hybrids: Loss of Mouse and Human Chromosomes

1998 ◽  
Vol 24 (3) ◽  
pp. 165-171 ◽  
Author(s):  
Xianghong Wang ◽  
Margaret Fox ◽  
Susan Povey ◽  
John R.W. Masters
Keyword(s):  
Somatic Cell ◽  
Human Chromosomes ◽  
Somatic Cell Hybrids ◽  
Human Somatic Cell ◽  
Cell Hybrids

scholarly journals Human Chromosomes 9, 12, and 15 Contain the Nucleation Sites of Stress-Induced Nuclear Bodies

2002 ◽  
Vol 13 (6) ◽  
pp. 2069-2079 ◽  
Author(s):  
Marco Denegri ◽  
Daniela Moralli ◽  
Mariano Rocchi ◽  
Marco Biggiogera ◽  
Elena Raimondi ◽  
...  
Keyword(s):  
Heat Shock ◽  
Human Cell ◽  
Hela Cells ◽  
Heat Shock Factor ◽  
Nuclear Bodies ◽  
Chromosome 9 ◽  
Heat Shock Factor 1 ◽  
Human Chromosomes ◽  
Cell Hybrids ◽  
Nucleation Sites

We previously reported the identification of a novel nuclear compartment detectable in heat-shocked HeLa cells that we termed stress-induced Src-activated during mitosis nuclear body (SNB). This structure is the recruitment center for heat shock factor 1 and for a number of RNA processing factors, among a subset of Serine-Arginine splicing factors. In this article, we show that stress-induced SNBs are detectable in human but not in hamster cells. By means of hamster>human cell hybrids, we have identified three human chromosomes (9, 12, and 15) that are individually able to direct the formation of stress bodies in hamster cells. Similarly to stress-induced SNB, these bodies are sites of accumulation of hnRNP A1-interacting protein and heat shock factor 1, are usually associated to nucleoli, and consist of clusters of perichromatin granules. We show that the p13-q13 region of human chromosome 9 is sufficient to direct the formation of stress bodies in hamster>human cell hybrids. Fluorescence in situ hybridization experiments demonstrate that the pericentromeric heterochromatic q12 band of chromosome 9 and the centromeric regions of chromosomes 12 and 15 colocalize with stress-induced SNBs in human cells. Our data indicate that human chromosomes 9, 12, and 15 contain the nucleation sites of stress bodies in heat-shocked HeLa cells.

Progress in the molecular cytogenetics of man

1988 ◽  
Vol 319 (1194) ◽  
pp. 239-248 ◽  
Keyword(s):  
Dna Sequences ◽  
Recombinant Dna ◽  
Molecular Cytogenetics ◽  
Chromosome Analysis ◽  
Parental Origin ◽  
Human Chromosomes ◽  
Recombinant Dna Technology ◽  
Chromosome Deletions ◽  
Dna Technology

Recombinant DNA technology has contributed greatly to the precision of chromosome analysis in man. Breakpoints of chromosome deletions and rearrangements may be defined on a chromosome map whose landmarks are the loci of DNA sequences rather than Giemsa bands. Flow cytogenetics allows the extent of chromosome duplications and deletions to be measured more precisely than has hitherto been possible. DNA probes can reveal hidden translocations through the application of in situ hybridization, and may be used as markers to determine the parental origin of non-disjunction. It is evident that a study of the pathology of human chromosomes now requires the combined skills of recombinant DNA and cytology.

scholarly journals Multiple human beta interferon genes.

1982 ◽  
Vol 156 (3) ◽  
pp. 744-755 ◽  
Author(s):  
A D Sagar ◽  
P B Sehgal ◽  
D L Slate ◽  
F H Ruddle
Keyword(s):  
Somatic Cell ◽  
Human Chromosomes ◽  
Somatic Cell Hybrids ◽  
Diploid Fibroblasts ◽  
Mrna Species ◽  
Human Diploid Fibroblasts ◽  
Beta Interferon ◽  
Cell Hybrids ◽  
Marked Cell ◽  
Mouse Somatic Cell

Analysis of human beta interferon (IFN) mRNA preparations obtained from poly(I) . poly (C)-induced human diploid fibroblasts (FS-4) and from several similarly induced human-mouse somatic cell hybrids by electrophoresis through agarose-CH3HgOH tube gels led to the detection of at least five translationally active human IFN-beta mRNA species. The results obtained are consistent with the existence of IFN-beta genes on different human chromosomes. Marked cell-dependent variability in the expression of these IFN mRNA species was observed.

PCR primers for human chromosomes: Reagents for the rapid analysis of somatic cell hybrids

Genomics ◽  
1991 ◽  
Vol 9 (3) ◽  
pp. 511-516 ◽  
Author(s):  
S. Theune ◽  
J. Fung ◽  
S. Todd ◽  
A.Y. Sakaguchi ◽  
S.L. Naylor
Keyword(s):  
Somatic Cell ◽  
Pcr Primers ◽  
Rapid Analysis ◽  
Human Chromosomes ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids

Positioning of human chromosomes in murine cell hybrids according to synteny

Chromosoma ◽  
2008 ◽  
Vol 117 (6) ◽  
pp. 579-591 ◽  
Author(s):  
Karen J. Meaburn ◽  
Robert F. Newbold ◽  
Joanna M. Bridger
Keyword(s):  
Human Chromosomes ◽  
Murine Cell ◽  
Cell Hybrids
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