Insulin-sensitivity and insulin-insensitivity in indian diabetics as determined by the insulin tolerance test and their influence on the therapeutic response to tolbutamide

1970 ◽  
Vol 7-7 (1) ◽  
pp. 767-776
Author(s):  
Inder Singh ◽  
Munish Chandra Srivastava
Keyword(s):  
Insulin Sensitivity ◽  
Therapeutic Response ◽  
Tolerance Test ◽  
Insulin Tolerance Test ◽  
Insulin Tolerance ◽  
Insulin Insensitivity

Comparison of the rapid insulin sensitivity test (RIST), the insulin tolerance test (ITT), and the hyperinsulinemic euglycemic clamp (HIEC) to measure insulin action in rats

2002 ◽  
Vol 80 (8) ◽  
pp. 811-818 ◽  
Author(s):  
Maria A.G Reid ◽  
Martin G Latour ◽  
Dallas J Legare ◽  
Na Rong ◽  
W Wayne Lautt
Keyword(s):  
Insulin Resistance ◽  
Insulin Sensitivity ◽  
Insulin Action ◽  
Initial Response ◽  
Tolerance Test ◽  
Insulin Tolerance Test ◽  
Sensitivity Test ◽  
Euglycemic Clamp ◽  
Hyperinsulinemic Euglycemic Clamp ◽  
Insulin Tolerance

The objective was to compare the ability of the rapid insulin sensitivity test (RIST), the hyperinsulinemic euglycemic clamp (HIEC), and the insulin tolerance test (ITT) to detect hepatic insulin sensitizing substance (HISS) dependent insulin action. HISS action was augmented by feeding and inhibited by fasting, blockade of hepatic nitric oxide synthase, or blockade of hepatic muscarinic cholinergic receptors. A significant correlation was found between the RIST index and ITT nadir (r2 = 0.84) but not between the glucose infusion rate of the HIEC and RIST index. There was, however, a relationship between the RIST index and the initial response during the HIEC. Use of the HIEC resulted in HISS-dependent insulin resistance in both conscious and anesthetized animals. We concluded that since the RIST and ITT were comparable in quantifying both HISS-dependent and HISS-independent insulin action, the RIST was validated against this standard. The observation that the HIEC is capable of detecting HISS action in the first rising slope of the test but not at the end of the test and that HISS release is fully blocked after the conclusion of the HIEC raises concerns about the use of the commonly used HIEC.Key words: HISS, insulin resistance, insulin sensitivity tests.

Evaluation of different methods for assessment of insulin sensitivity in Göttingen minipigs: introduction of a new, simpler method

2009 ◽  
Vol 297 (4) ◽  
pp. R1195-R1201 ◽  
Author(s):  
Berit Christoffersen ◽  
Ulla Ribel ◽  
Kirsten Raun ◽  
Valeria Golozoubova ◽  
Giovanni Pacini
Keyword(s):  
Insulin Sensitivity ◽  
Cell Function ◽  
High Energy ◽  
Tolerance Test ◽  
Insulin Tolerance Test ◽  
Oral Glucose ◽  
Β Cell ◽  
Simple Index ◽  
Insulin Tolerance ◽  
Β Cell Function

The use of animal models in diabetes research requires reliable tests for evaluation of insulin sensitivity and β-cell function. Minipigs are being increasingly used in metabolic research, and the aim of this study was to compare different tests and indexes for evaluation of insulin sensitivity and β-cell function in Göttingen minipigs. Hyperinsulinemic, isoglycemic clamp, intravenous (IVGTT) and oral glucose tolerance tests (OGTT), and a modified insulin tolerance test were performed in minipigs fed either low- or high-energy diet. Furthermore, the reproducibility of IVGTT-derived parameters was assessed. Previously described insulin sensitivity indexes [steady-state glucose infusion rate/glucose concentration/insulin concentration from clamp (M/G/I); oral glucose insulin sensitivity (OGIS) and ISIcomp from OGTT; SI from minimal model analysis of IVGTT; and quantitative insulin sensitivity check index from fasting values] were calculated together with an insulin sensitivity index from the modified insulin tolerance test (ISIITT) and a new simple index (S2) derived from the first 30 min of the IVGTT. β-Cell function was assessed from the IVGTT and the OGTT. Reproducibility of the IVGTT-derived parameters was calculated as median intraindividual coefficient of variation (CV%).M/G/I correlated significantly only with S2 ( P < 0.05, r = 0.54). S2 furthermore correlated with SI ( P < 0.001, r = 0.81), ISIITT ( P < 0.001, r = 0.57), and the two indexes from OGTT, ISIcomp ( P < 0.001, r = 0.78) and OGIS ( p < 0.05, r = 0.48). No correlation was found between β-cell function indexes from OGTT and IVGTT. The median CV% of the new S2 index was 13. In conclusion, the new simple index of insulin sensitivity, S2, was revealed to be useful for evaluation of insulin sensitivity in pigs.

Validation of the low dose short insulin tolerance test for evaluation of insulin sensitivity

1994 ◽  
Vol 40 (5) ◽  
pp. 611-615 ◽  
Author(s):  
S. V. Gelding ◽  
S. Robinson ◽  
S. Lowe ◽  
R. Niththyananthan ◽  
D. G. Johnston
Keyword(s):  
Insulin Sensitivity ◽  
Low Dose ◽  
Tolerance Test ◽  
Insulin Tolerance Test ◽  
Insulin Tolerance
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