Adenosine kinase as a new selective marker in somatic cell genetics: Isolation of adenosine kinase-deficient mouse cell lines and human-mouse hybrid cell lines containing adenosine kinase

1978 ◽  
Vol 4 (1) ◽  
pp. 1-12 ◽  
Author(s):  
Teh-sheng Chan ◽  
Richard P. Creagan ◽  
M. Patricia Reardon
Keyword(s):  
Somatic Cell ◽  
Cell Lines ◽  
Hybrid Cell ◽  
Mouse Cell ◽  
Adenosine Kinase ◽  
Deficient Mouse ◽  
Selective Marker ◽  
Somatic Cell Genetics ◽  
Hybrid Cell Lines

Genetic analysis of the human Y chromosome by chromosome-mediated gene transfer

Development ◽  
1987 ◽  
Vol 101 (Supplement) ◽  
pp. 59-65
Author(s):  
Catrin A. Pritchard ◽  
Peter N. Goodfellow
Keyword(s):  
Gene Transfer ◽  
Y Chromosome ◽  
Cell Lines ◽  
Selectable Marker ◽  
Hybrid Cell ◽  
Mapping Data ◽  
Somatic Cell Genetics ◽  
Structural Mapping ◽  
Hybrid Cell Lines ◽  
Human Y Chromosome

Chromosome-mediated gene transfer (CMGT) can be used to segregate fragments of human chromosomes in human—rodent hybrid cells. As with all somatic cell genetics methods, a selection technique is needed to isolate the hybrid cell lines produced by CMGT. Expression of the MIC2 gene product on the cell surface (the 12E7 antigen) provides an endogenous selectable marker for the human Y chromosome. Using chromosome transfer followed by separation of 12E7 antigen-positive cells on the fluorescence-activated cell sorter, a series of cell lines containing segregated fragments of the Y chromosome have been derived. The possibility of using these fragments to derive fine structural mapping data for the Y chromosome is considered in this review.

Selectable mutations altering two mechanisms of mammalian K+ transport are dominant

1987 ◽  
Vol 252 (5) ◽  
pp. C515-C522 ◽  
Author(s):  
J. J. Gargus
Keyword(s):  
Somatic Cell ◽  
Cell Lines ◽  
Selectable Marker ◽  
Hybrid Cell ◽  
Mutant Phenotype ◽  
Wild Type ◽  
Low Potassium ◽  
Cotransport System ◽  
Hybrid Cell Lines ◽  
K Transport

Somatic cell mutants with altered K+ transport have previously been isolated from mutagenzied LMTK- cells for their ability to grow at subthreshold low-potassium concentrations (0.2 mM). These mutants fall into two classes: one class, LTK-5, possesses a functionally altered furosemide-sensitive Na+-K+-Cl- cotransport system and the other, LTK-1, an altered K+-conducting channel. Somatic cell hybrids have been formed between each of these cell lines and a wild-type L-cell line, making use of complementing selectable marker mutations carried by these parents, to establish the dominance of the K+ transport mutations. Hybrids were isolated and studied in two ways: clonal hybrid cell lines were selected in a manner unbiased toward their K+ transport phenotype, which was later assayed; and the number of independent hybrids arising in this single-selective condition was compared with the number arising in a condition which is double selective for the mutant phenotype as well. By both assays, hybrids formed with LTK-1 or LTK-5 as a parent uniformly exhibited the mutant phenotype by growth and cloning, whereas control hybrids with LMTK- as parent never did. This demonstrates both transport mutations to be dominant and thus potentially isolatable.

Extinction and activation of the thyroglobulin promoter in hybrids of differentiated and transformed thyroid cells

1990 ◽  
Vol 10 (3) ◽  
pp. 1033-1040
Author(s):  
I M Bonapace ◽  
M Sanchez ◽  
S Obici ◽  
A Gallo ◽  
S Garofalo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Lines ◽  
Selectable Marker ◽  
Hybrid Cell ◽  
Transformed Cells ◽  
Thyroid Cells ◽  
Dominant Selectable Marker ◽  
Thyroglobulin Gene ◽  
Rat Thyroid ◽  
Hybrid Cell Lines

Thyroglobulin gene expression was repressed in a rat thyroid cell line transformed with Kirsten murine sarcoma virus. Expression of a dominant selectable marker driven by the thyroglobulin promoter was also inhibited. Somatic cell hybridization of transformed and differentiated thyroid cells resulted in extinction of thyroglobulin gene expression. When transformed cells carrying a dominant selectable marker driven by the thyroglobulin promoter were fused to differentiated cells and expression of this marker was selected, we obtained stable hybrid cell lines expressing both the endogenous and the exogenous thyroglobulin promoters. Although the expression of v-ras remained unchanged compared with expression in the parental transformed cells, transformation was suppressed in the hybrid cell lines. The other thyroid differentiation markers, iodide uptake and thyroid-stimulating hormone-dependent growth, were inhibited in all the hybrids tested. We show that activity of the thyroglobulin promoter correlates with the presence of a thyroid nuclear factor that binds the promoter at position -60 from the transcription start site. Loss of this factor accompanies the extinction of thyroglobulin gene expression in hybrids selected for expression of a non-thyroid-specific promoter.

Embryonic stem cells can be used to construct hybrid cell lines containing a single, selectable murine chromosome

1999 ◽  
Vol 10 (4) ◽  
pp. 381-384 ◽  
Author(s):  
Petra M. Jakobs ◽  
Lesley Smith ◽  
Mathew Thayer ◽  
Markus Grompe
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Cell Lines ◽  
Hybrid Cell ◽  
Embryonic Stem ◽  
Murine Chromosome ◽  
Hybrid Cell Lines

scholarly journals Propagation of Hepatitis A Virus in Hybrid Cell Lines Derived from Marmoset Liver and Vero Cells

1989 ◽  
Vol 70 (9) ◽  
pp. 2487-2494 ◽  
Author(s):  
M. Ashida ◽  
H. Hara ◽  
H. Kojima ◽  
T. Kamimura ◽  
F. Ichida ◽  
...  
Keyword(s):  
Cell Lines ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Hybrid Cell ◽  
Vero Cells ◽  
Hybrid Cell Lines
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