Staphylococcus aureus Cowan I-induced human immunoglobulin responses: Preferential IgM rheumatoid factor production and VH3 mRNA expression by protein A-binding B cells

1995 ◽  
Vol 15 (3) ◽  
pp. 145-151 ◽  
Author(s):  
L. M. Kozlowski ◽  
S. Roman Running ◽  
Y. Zheng ◽  
L. M. Wheatley ◽  
A. I. Levinson
Keyword(s):  
Staphylococcus Aureus ◽  
B Cells ◽  
Rheumatoid Factor ◽  
Mrna Expression ◽  
Protein A ◽  
Human Immunoglobulin ◽  
Igm Rheumatoid Factor ◽  
Factor Production ◽  
Staphylococcus Aureus Cowan ◽  
Staphylococcus Aureus Cowan I

scholarly journals Analysis of protein A encoded by a mutated gene of Staphylococcus aureus Cowan I

1985 ◽  
Vol 153 (3) ◽  
pp. 579-585 ◽  
Author(s):  
Bengt GUSS ◽  
Karen LEANDER ◽  
Ulf HELLMAN ◽  
Mathias UHLEN ◽  
John SJOQUIST ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Protein A ◽  
Staphylococcus Aureus Cowan ◽  
Staphylococcus Aureus Cowan I

scholarly journals Expression of Tac antigen on activated normal human B cells.

1984 ◽  
Vol 160 (2) ◽  
pp. 612-617 ◽  
Author(s):  
M Tsudo ◽  
T Uchiyama ◽  
H Uchino
Keyword(s):  
Staphylococcus Aureus ◽  
B Cells ◽  
Interleukin 2 ◽  
Fluorescence Analysis ◽  
Cell Immune Response ◽  
Receptor System ◽  
Human B Cells ◽  
Staphylococcus Aureus Cowan ◽  
Normal Human ◽  
Staphylococcus Aureus Cowan I

Two-color fluorescence analysis revealed that Tac antigen, which was previously reported to be restricted to T cells, was expressed on a proportion of normal B cells activated by Staphylococcus aureus Cowan I (SAC). Immunoaffinity-purified interleukin 2 (IL-2) induced the proliferation of SAC-activated B cells, and the proliferation was completely inhibited by anti-Tac antibody, which blocked the membrane binding and action of IL-2. These results suggest that an IL-2 receptor system is directly involved in the B cell immune response.

scholarly journals Interleukin 2-induced proliferation of leukemic human B cells.

1985 ◽  
Vol 161 (5) ◽  
pp. 1225-1230 ◽  
Author(s):  
O Lantz ◽  
C Grillot-Courvalin ◽  
C Schmitt ◽  
J P Fermand ◽  
J C Brouet
Keyword(s):  
Staphylococcus Aureus ◽  
T Cells ◽  
B Cells ◽  
B Cell ◽  
Proliferative Response ◽  
Interleukin 2 ◽  
Human B Cells ◽  
Staphylococcus Aureus Cowan ◽  
Recombinant Interleukin 2 ◽  
Staphylococcus Aureus Cowan I

The proliferative responses of purified leukemic human B cells from nine B cell chronic lymphocytic leukemias to recombinant interleukin 2 (IL-2), spontaneously, and after preactivation by Staphylococcus aureus Cowan I (SAC) or anti-mu antibodies were studied. Three patterns of response were observed: (a) no response (three cases); (b) a moderate spontaneous response enhanced by anti-mu (one case); (c) a high proliferative response after preactivation by anti-mu and/or SAC (five cases). IL-2 could also trigger normal B cells, purified from spleen, to proliferative after preactivation by anti-mu or SAC. These results provide evidence that IL-2 is a lymphokine that acts physiologically on both B and T cells.

scholarly journals Production of Antibodies in Egg Whites of Chickens

2021 ◽  
pp. 17-22
Author(s):  
Angel Justiz Vaillant ◽  
Belkis Ferrer-Cosme ◽  
Sehlule Vuma
Keyword(s):  
Staphylococcus Aureus ◽  
Affinity Chromatography ◽  
Protein A ◽  
Primary Immune Response ◽  
Bacterial Antigen ◽  
Staphylococcal Protein A ◽  
Staphylococcal Protein ◽  
Staphylococcus Aureus Cowan ◽  
Staphylococcus Aureus Cowan I

Background:  IgM, which participates in the primary immune response, is the primary antibody in egg whites. There is scant information about the production of antibodies in egg whites. This study describes the preparation of antibodies against a bacterial antigen, staphylococcal protein-A. Methods: The detection of antibodies against staphylococcal protein-A in egg white was performed by ELISA, and the antibodies were purified by protein-A affinity chromatography. Agglutination inhibition of Staphylococcus aureus Cowan I strains by purified antibodies against protein-A in vitro was investigated. Results:  ELISA showed the production of antibodies against staphylococcal protein-A in the egg whites of layer hens. The antibodies were separated using affinity chromatography. The agglutination of Staphylococcus aureus Cowan I strains occurred when the purified antibodies were incubated with S. aureus. Conclusion: The results showed that it is possible to produce antibodies against bacterial antigens in egg whites, which can have industrial applications in the preparation of antibodies for immunotherapy of infectious diseases.

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