Regional mapping of human genes for hexosaminidase B and diphtheria toxin sensitivity on chromosome 5 using mouse � human hybrid cells

1977 ◽  
Vol 3 (6) ◽  
pp. 629-638 ◽  
Author(s):  
Donna L. George ◽  
Uta Francke
Keyword(s):  
Diphtheria Toxin ◽  
Regional Mapping ◽  
Hybrid Cells ◽  
Chromosome 5 ◽  
Human Genes ◽  
Toxin Sensitivity

Sensitivity to Diphtheria Toxin as a Species-Specific Marker in Hybrid Cells

1973 ◽  
Vol 12 (3) ◽  
pp. 831-837 ◽  
Author(s):  
P. R. DENDY ◽  
H. HARRIS
Keyword(s):  
Interspecific Hybrid ◽  
Diphtheria Toxin ◽  
Specific Marker ◽  
Hybrid Cells ◽  
Species Specific

Diphtheria toxin may be used as a species-specific marker in the study of interspecific hybrid cells. Sensitivity to the toxin is dominant in heterokaryons made by fusing sensitive and insensitive cells.

Regional assignment of the gene for diphtheria toxin sensitivity using subchromosomal fragments in microcell hybrids

Chromosoma ◽  
1987 ◽  
Vol 96 (1) ◽  
pp. 26-32 ◽  
Author(s):  
H�l�ne Hayes ◽  
Yasufumi Kaneda ◽  
Tsuyoshi Uchida ◽  
Yoshio Okada
Keyword(s):  
Diphtheria Toxin ◽  
Toxin Sensitivity ◽  
Microcell Hybrids ◽  
Regional Assignment

scholarly journals SURFACE ANTIGENS OF MAMMALIAN CELLS AS GENETIC MARKERS. II

1973 ◽  
Vol 138 (1) ◽  
pp. 229-244 ◽  
Author(s):  
Paul Wuthier ◽  
Carol Jones ◽  
Theodore T. Puck
Keyword(s):  
Mammalian Cells ◽  
Human Lymphocytes ◽  
Human Fibroblasts ◽  
Chinese Hamster ◽  
Lactic Dehydrogenase ◽  
Hybrid Cells ◽  
Human Genes ◽  
Cultured Human Fibroblasts ◽  
Human Rbcs ◽  
Cell Plating

A second surface antigen, BL, lethal in the presence of specific antibody and complement has been identified on some human cells and shown to behave as a good genetic marker. It is autosomal, unlinked to the human AL antigen previously described, and unlinked to 15 other human genes. The AL antigen, which is linked to the lactic dehydrogenase A gene, is found on the HeLa, the cultured human fibroblast, and in small amounts on the human lymphocyte. BL occurs on HeLa cells, on cultured human fibroblasts, and on human lymphocytes, but not on human RBCs. Hybrid cells formed by fusion of human and Chinese hamster cells have been prepared containing each of the four possible combinations of these two markers. Highly selective antisera sensitive to each marker separately can be obtained. The use of single-cell plating to demonstrate the presence of the antigens and of hybrid cells containing desired combinations of the markers facilitates study in this system.

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