Nucleocytoplasmic interactions in experimental binucleates formed from normal and transformed components

1979 ◽  
Vol 5 (3) ◽  
pp. 397-407 ◽  
Author(s):  
Audrey L. Muggleton-Harris ◽  
Michael Palumbo
Keyword(s):  
Nucleocytoplasmic Interactions

scholarly journals PROTEINS IN NUCLEOCYTOPLASMIC INTERACTIONS

1968 ◽  
Vol 39 (2) ◽  
pp. 404-414 ◽  
Author(s):  
David Prescott ◽  
Lester Goldstein
Keyword(s):  
Binding Sites ◽  
Interphase Nucleus ◽  
Nuclear Protein ◽  
Protein A ◽  
Amoeba Proteus ◽  
Nuclear Proteins ◽  
Nuclear Transplantation ◽  
Amino Acid Labeling ◽  
Turn Over ◽  
Nucleocytoplasmic Interactions

The behavior of nuclear proteins in Amoeba proteus was studied by tritiated amino acid labeling, nuclear transplantation, and cytoplasmic amputation. During prophase at least 77% (but probably over 95%) of the nuclear proteins is released to the cytoplasm. These same proteins return to the nucleus within the first 3 hr of interphase. When cytoplasm is amputated from an ameba in mitosis (shen the nuclear proteins are in the cytoplasm), the resultant daughter nuclei are depleted in the labeled nuclear proteins. The degree of depletion is less than proportional to the amount of cytoplasm removed because a portion of rapidly migrating protein (a nuclear protein that is normally shuttling between nucleus and cytoplasm and is thus also present in the cytoplasm) which would normally remain in the cytoplasm is taken up by the reconstituting daughter nuclei. Cytoplasmic fragments cut from mitotic cells are enriched in both major classes of nuclear proteins, i.e. rapidly migrating protein and slow turn-over protein. An interphase nucleus implanted into such an enucleated cell acquires from the cytoplasm essentially all of the excess nuclear proteins of both classes. The data indicate that there is a lack of binding sites in the cytoplasm for the rapidly migrating nuclear protein. The quantitative aspects of the distribution of rapidly migrating protein between the nucleus and the cytoplasm indicate that the distribution is governed primarily by factors within the nucleus.

Studies on an embryonic lethal hybrid in Drosophila

Development ◽  
1967 ◽  
Vol 17 (2) ◽  
pp. 405-423
Author(s):  
Janice D. Kinsey
Keyword(s):  
X Chromosome ◽  
Interspecific Hybrids ◽  
Genetic Mechanism ◽  
Abnormal Development ◽  
Central Importance ◽  
Embryonic Lethal ◽  
Female Hybrid ◽  
Drosophila Montana ◽  
Lethal Hybrid ◽  
Nucleocytoplasmic Interactions

The problems of nucleocytoplasmic interactions are of central importance in development and genetics. Studies of such interactions may help elucidate the mechanisms of differentiation of cells receiving the same genetic complement. One approach to a study of nucleocytoplasmic interactions is by examination of the development of interspecific hybrids, particularly those in which either syngamy fails to occur, or an abnormal development is produced. These studies describe a lethal hybrid which occurs between Drosophila montana and D. texana. In 1944, Patterson & Griffen described a genetic mechanism which acts in the hybrid females produced by crossing D. montana females to D. texana males. In this cross, only male offspring were produced, so that there appeared to be some incompatibility between the D. montana ooplasm and the D. texana X-chromosome which acted to kill the female hybrid embryos before hatching.

scholarly journals PROTEINS IN NUCLEOCYTOPLASMIC INTERACTIONS

1971 ◽  
Vol 48 (1) ◽  
pp. 202-207 ◽  
Author(s):  
Samar Chatterjee ◽  
Lester Goldstein
Keyword(s):  
Nucleocytoplasmic Interactions
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