Effects of alcohol on lectin binding affinity in rat gastric mucosa

1990 ◽  
Vol 35 (7) ◽  
pp. 865-872 ◽  
Author(s):  
Philip A. Mitchell ◽  
Thomas A. Miller ◽  
Karmen L. Schmidt
Keyword(s):  
Gastric Mucosa ◽  
Binding Affinity ◽  
Lectin Binding ◽  
Rat Gastric Mucosa

scholarly journals Reaction of Various Lectins to Mucin Derived from the Different Layers of Rat Gastric Mucosa. Comparison of Enzyme-Linked Lectin Binding Assay with Lectin Histochemistry.

2000 ◽  
Vol 23 (10) ◽  
pp. 1173-1179 ◽  
Author(s):  
Hisamasa KODAIRA ◽  
Kazuhiko ISHIHARA ◽  
Kyoko HOTTA ◽  
Masatoyo KAGOSHIMA ◽  
Hideyo SHIMADA ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Binding Assay ◽  
Lectin Binding ◽  
Lectin Histochemistry ◽  
Rat Gastric Mucosa

scholarly journals Characterization of glycoconjugates of human gastrointestinal mucosa by lectins. II. Lectin binding to the isolated glycoproteins of normal and malignant gastric mucosa.

1984 ◽  
Vol 32 (7) ◽  
pp. 690-696 ◽  
Author(s):  
J Fischer ◽  
G Uhlenbruck ◽  
P J Klein ◽  
M Vierbuchen ◽  
R Fischer
Keyword(s):  
Molecular Weight ◽  
Gastric Mucosa ◽  
High Molecular Weight ◽  
Lectin Binding ◽  
Gel Filtration ◽  
Molar Ratio ◽  
Gastric Cancer Cells ◽  
Tissue Sections ◽  
Gradient Gel Electrophoresis ◽  
Triton X

Using affinity chromatography on HPA-, PNA-, Con A, and WGA-agarose columns only a part (10-30%) of the high molecular weight mucous glycoproteins could be isolated from the Triton X-100 solubilized components of normal as well as carcinomatous gastric mucosa. The main part of the mucus was not bound by the lectins, which corresponds to our earlier lectin histochemical observations on paraffin-embedded tissue sections. The lectin-bound mucous glycoproteins had a relatively lower molecular weight, ranging from about 250-1,000 kilodaltons, as indicated by polyacrylamide gradient gel electrophoresis and by gel filtration on Biogel A 1.5 m column. In gas chromatographic analysis the molar ratio of aminohexoses to galactose was found to be much higher (3:1) in the lectin-bound mucous substances than in the whole high molecular weight mucus (1:1). This finding indicates that lectins have a higher affinity to the hexosamine rich components of mucus, which may be special forms of mucous glycoprotein molecules or the incompletely glycosylated core and backbone regions of the oligosaccharide chains of mucus. Extremely high hexosamine values (10:1) were found in the PNA isolated mucus of gastric adenocarcinoma. Since it is known that PNA binds to the terminal disaccharide, beta-galactose-(1-3)-N-acetylgalactosamine, which is localized at the reducing end of the oligosaccharide chains of mucus, it is highly probable that the elongation of the oligosaccharide side chains is disturbed in gastric cancer cells.

Role of complement regulatory membrane proteins in ischaemia-reperfusion injury of rat gastric mucosa

1999 ◽  
Vol 14 (10) ◽  
pp. 967-972 ◽  
Author(s):  
Fumihiro Iwata ◽  
Takashi Joh ◽  
Toyohiro Tada ◽  
Noriko Okada ◽  
B Paul Morgan ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Membrane Proteins ◽  
Reperfusion Injury ◽  
Ischaemia Reperfusion Injury ◽  
Ischaemia Reperfusion ◽  
Rat Gastric Mucosa

The lectin binding affinity of Reed-Sternberg cells

1983 ◽  
Vol 139 (2) ◽  
pp. 151-158 ◽  
Author(s):  
Christine S. Morris ◽  
A. E. Stuart
Keyword(s):  
Binding Affinity ◽  
Lectin Binding
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