Long-range restriction map around 11p13 aniridia locus

1989 ◽  
Vol 15 (6) ◽  
pp. 605-615 ◽  
Author(s):  
Lisa M. Davis ◽  
Andrew M. Everest ◽  
Kalle O. J. Simola ◽  
Thomas B. Shows
Keyword(s):  
Long Range ◽  
Restriction Map ◽  
Range Restriction

scholarly journals CpG island mapping of a mouse double-minute chromosome.

1993 ◽  
Vol 13 (8) ◽  
pp. 4459-4464 ◽  
Author(s):  
J L Beland ◽  
J A Longo ◽  
P J Hahn
Keyword(s):  
Cell Line ◽  
Long Range ◽  
Molecular Mechanisms ◽  
Cpg Island ◽  
Single Copy ◽  
Restriction Map ◽  
Chromosomal Dna ◽  
Dhfr Gene ◽  
Range Restriction ◽  
Double Minute

The development of double-minute chromosomes (DMs) and subsequent gene amplification are important genomic alterations resulting in increased oncogene expression in a variety of tumors. The molecular mechanisms mediating the development of these acentric extrachromosomal elements have not been completely defined. To elucidate the mechanisms involved in DM formation, we have developed strategies to map amplified circular DM DNA. In this study, we present a long-range restriction map of a 980-kb DM. A cell line cloned from mouse EMT-6 cells was developed by stepwise selection for resistance to methotrexate. This cloned cell line contains multiple copies of the 980-kb DM carrying the dihydrofolate reductase (DHFR) gene. A long-range restriction map was developed in which a hypomethylated CpG-rich region near the DHFR gene served as a landmark. This strategy was combined with plasmid-like analysis of ethidium bromide-stained pulsed-field gels and indicated that a single copy of the DHFR gene was located near a hypomethylated region containing SsII and NotI sites. At least 490 kb of this DM appears to be composed of unrearranged chromosomal DNA.

CpG island mapping of a mouse double-minute chromosome

1993 ◽  
Vol 13 (8) ◽  
pp. 4459-4464
Author(s):  
J L Beland ◽  
J A Longo ◽  
P J Hahn
Keyword(s):  
Cell Line ◽  
Long Range ◽  
Molecular Mechanisms ◽  
Cpg Island ◽  
Single Copy ◽  
Restriction Map ◽  
Chromosomal Dna ◽  
Dhfr Gene ◽  
Range Restriction ◽  
Double Minute

The development of double-minute chromosomes (DMs) and subsequent gene amplification are important genomic alterations resulting in increased oncogene expression in a variety of tumors. The molecular mechanisms mediating the development of these acentric extrachromosomal elements have not been completely defined. To elucidate the mechanisms involved in DM formation, we have developed strategies to map amplified circular DM DNA. In this study, we present a long-range restriction map of a 980-kb DM. A cell line cloned from mouse EMT-6 cells was developed by stepwise selection for resistance to methotrexate. This cloned cell line contains multiple copies of the 980-kb DM carrying the dihydrofolate reductase (DHFR) gene. A long-range restriction map was developed in which a hypomethylated CpG-rich region near the DHFR gene served as a landmark. This strategy was combined with plasmid-like analysis of ethidium bromide-stained pulsed-field gels and indicated that a single copy of the DHFR gene was located near a hypomethylated region containing SsII and NotI sites. At least 490 kb of this DM appears to be composed of unrearranged chromosomal DNA.

A long-range restriction map of the interleukin-4 and interleukin-5 linkage group on chromosome 5

Genomics ◽  
1990 ◽  
Vol 6 (1) ◽  
pp. 94-99 ◽  
Author(s):  
Settara C. Chandrasekharappa ◽  
Michelle S. Rebelsky ◽  
Thomas A. Firak ◽  
Michelle M. Le Beau ◽  
Carol A. Westbrook
Keyword(s):  
Linkage Group ◽  
Long Range ◽  
Interleukin 4 ◽  
Restriction Map ◽  
Chromosome 5 ◽  
Range Restriction ◽  
Interleukin 5

A long-range restriction map encompassing the cystic fibrosis locus and its closely linked genetic markers

Genomics ◽  
1988 ◽  
Vol 2 (4) ◽  
pp. 337-345 ◽  
Author(s):  
AnneMarie Poustka ◽  
Hans Lehrach ◽  
Robert Williamson ◽  
Gillian Bates
Keyword(s):  
Cystic Fibrosis ◽  
Long Range ◽  
Genetic Markers ◽  
Restriction Map ◽  
Range Restriction

A long-range restriction map across 3 Mb of the chromosome 11 breakpoint region of a translotion linked to schizophrenia

1998 ◽  
Vol 8 (3) ◽  
pp. 175-182 ◽  
Author(s):  
J. K. Millar ◽  
J. Brown ◽  
J. C. Maule ◽  
Y. Shibasaki ◽  
S. Christie ◽  
...  
Keyword(s):  
Long Range ◽  
Breakpoint Region ◽  
Restriction Map ◽  
Chromosome 11 ◽  
Range Restriction

Long range restriction map of the von Hippel-Lindau gene region on human chromosome 3p

1993 ◽  
Vol 92 (3) ◽  
Author(s):  
SusanneC. Szymanski ◽  
Holger Hummerich ◽  
Farida Latif ◽  
MichaelI. Lerman ◽  
Gunter R�hrborn ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Long Range ◽  
Gene Region ◽  
Restriction Map ◽  
Range Restriction ◽  
Von Hippel Lindau ◽  
Chromosome 3P
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