Synthesis and incorporation of human ribosomal protein S14 into functional ribosomes in human-Chinese hamster cell hybrids containing human chromosome 5: HumanRPS14 gene is the structural gene for ribosomal protein S14

1985 ◽  
Vol 11 (6) ◽  
pp. 625-631 ◽  
Author(s):  
Sharon L. Dana ◽  
Stephen Chang ◽  
John J. Wasmuth
Keyword(s):  
Human Chromosome ◽  
Ribosomal Protein ◽  
Structural Gene ◽  
Chinese Hamster ◽  
Chinese Hamster Cell ◽  
Chromosome 5 ◽  
Cell Hybrids ◽  
Human Ribosomal Protein ◽  
Human Chromosome 5

Selective linkage disruption in human-Chinese hamster cell hybrids: deletion mapping of the leuS, hexB, emtB, and chr genes on human chromosome 5

1982 ◽  
Vol 2 (10) ◽  
pp. 1220-1228
Author(s):  
S Dana ◽  
J J Wasmuth
Keyword(s):  
Human Chromosome ◽  
Mammalian Species ◽  
Chinese Hamster ◽  
Trna Synthetase ◽  
Gene Organization ◽  
Deletion Mapping ◽  
Chromosome 2 ◽  
Chromosome 5 ◽  
Human Chromosome 5 ◽  
Chinese Hamster Chromosome

Chinese hamster-human interspecific hybrid cells, which contain human chromosome 5 and express four genes linked on that chromosome, were subjected to selective conditions requiring them to retain one of the four linked genes, leuS (encoding leucyl-tRNA synthetase), but lose another, either emtB (encoding ribosomal protein S14) or chr. Cytogenetic and biochemical analyses of spontaneous segregants isolated by using these unique selective pressures have enabled us to determine the order and regional location of the leuS, hexB, emtB, and chr genes on human chromosome 5. These segregants arise primarily by terminal deletions of various portions of the long arm of chromosome 5. Our results indicate that the order of at least three of these genes is the same on human chromosome 5 and Chinese hamster chromosome 2. Thus, there appears to be extensive homology between Chinese hamster chromosome 2 and human chromosome 5, which represents an extreme example of the conservation of gene organization between very divergent mammalian species. In addition, these hybrids and selective conditions provide a very simple and quantitative means to assess the potency of various agents suspected of inducing gross chromosomal damage.

scholarly journals Selective linkage disruption in human-Chinese hamster cell hybrids: deletion mapping of the leuS, hexB, emtB, and chr genes on human chromosome 5.

1982 ◽  
Vol 2 (10) ◽  
pp. 1220-1228 ◽  
Author(s):  
S Dana ◽  
J J Wasmuth
Keyword(s):  
Human Chromosome ◽  
Mammalian Species ◽  
Chinese Hamster ◽  
Trna Synthetase ◽  
Gene Organization ◽  
Deletion Mapping ◽  
Chromosome 2 ◽  
Chromosome 5 ◽  
Human Chromosome 5 ◽  
Chinese Hamster Chromosome

Chinese hamster-human interspecific hybrid cells, which contain human chromosome 5 and express four genes linked on that chromosome, were subjected to selective conditions requiring them to retain one of the four linked genes, leuS (encoding leucyl-tRNA synthetase), but lose another, either emtB (encoding ribosomal protein S14) or chr. Cytogenetic and biochemical analyses of spontaneous segregants isolated by using these unique selective pressures have enabled us to determine the order and regional location of the leuS, hexB, emtB, and chr genes on human chromosome 5. These segregants arise primarily by terminal deletions of various portions of the long arm of chromosome 5. Our results indicate that the order of at least three of these genes is the same on human chromosome 5 and Chinese hamster chromosome 2. Thus, there appears to be extensive homology between Chinese hamster chromosome 2 and human chromosome 5, which represents an extreme example of the conservation of gene organization between very divergent mammalian species. In addition, these hybrids and selective conditions provide a very simple and quantitative means to assess the potency of various agents suspected of inducing gross chromosomal damage.

Segregation of recessive phenotypes in somatic cell hybrids: role of mitotic recombination, gene inactivation, and chromosome nondisjunction

1981 ◽  
Vol 1 (4) ◽  
pp. 336-346
Author(s):  
C E Campbell ◽  
R G Worton
Keyword(s):  
Somatic Cell ◽  
Chinese Hamster ◽  
Mitotic Recombination ◽  
Chinese Hamster Cell ◽  
Gene Inactivation ◽  
Chromosome Loss ◽  
Resistance Locus ◽  
Chromosome 2 ◽  
Somatic Cell Hybrids ◽  
Cell Hybrids

Somatic cell hybrids heterozygous at the emetine resistance locus (emtr/emt+) or the chromate resistance locus (chrr/chr+) are known to segregate the recessive drug resistance phenotype at high frequency. We have examined mechanisms of segregation in Chinese hamster cell hybrids heterozygous at these two loci, both of which map to the long arm of Chinese hamster chromosome 2. To follow the fate of chromosomal arms through the segregation process, our hybrids were also heterozygous at the mtx (methotrexate resistance) locus on the short arm of chromosome 2 and carried cytogenetically marked chromosomes with either a short-arm deletion (2p-) or a long-arm addition (2q+). Karyotype and phenotype analysis of emetine- or chromate-resistant segregants from such hybrids allowed us to distinguish four potential segregation mechanisms: (i) loss of the emt+- or chr+-bearing chromosome; (ii) mitotic recombination between the centromere and the emt or chr loci, giving rise to homozygous resistant segregants; (iii) inactivation of the emt+ or chr+ alleles; and (iv) loss of the emt+- or chr+-bearing chromosome with duplication of the homologous chromosome carrying the emtr or chrr allele. Of 48 independent segregants examined, only 9 (20%) arose by simple chromosome loss. Two segregants (4%) were consistent with a gene inactivation mechanism, but because of their rarity, other mechanisms such as mutation or submicroscopic deletion could not be excluded. Twenty-one segregants (44%) arose by either mitotic recombination or chromosome loss and duplication; the two mechanisms were not distinguishable in that experiment. Finally, in hybrids allowing these two mechanisms to be distinguished, 15 segregants (31%) arose by chromosome loss and duplication, and none arose by mitotic recombination.

scholarly journals The gene coding for the p68 calcium-binding protein is localised to bands q32?q34 of human chromosome 5, and to mouse chromosome 11

1989 ◽  
Vol 82 (3) ◽  
pp. 234-238 ◽  
Author(s):  
Adelina A. Davies ◽  
Stephen E. Moss ◽  
Mark R. Crompton ◽  
Tania A. Jones ◽  
Nigel K. Spurr ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Mouse Chromosome ◽  
Calcium Binding ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
Chromosome 11 ◽  
Chromosome 5 ◽  
Gene Coding ◽  
Mouse Chromosome 11 ◽  
Human Chromosome 5

Genes encoding adrenergic receptors are not clustered on the long arm of human chromosome 5

1994 ◽  
Vol 67 (2) ◽  
pp. 69-74 ◽  
Author(s):  
S.K. Loftus ◽  
R. Shiang ◽  
J.A. Warrington ◽  
U. Bengtsson ◽  
J.D. McPherson ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Adrenergic Receptors ◽  
Chromosome 5 ◽  
Genes Encoding ◽  
Human Chromosome 5

IL9 maps to mouse chromosome 13 and human chromosome 5

1990 ◽  
Vol 31 (4) ◽  
pp. 265-270 ◽  
Author(s):  
Beverly A. Mock ◽  
Marianne Krall ◽  
Christine A. Kozak ◽  
Muriel N. Nesbitt ◽  
O. Wesley McBride ◽  
...  
Keyword(s):  
Human Chromosome ◽  
Mouse Chromosome ◽  
Chromosome 5 ◽  
Chromosome 13 ◽  
Human Chromosome 5
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