Effects of insulin, dexamethasone and cytokines on ?1-acid glycoprotein gene expression in primary cultures of normal rat hepatocytes

Inflammation ◽  
1996 ◽  
Vol 20 (2) ◽  
pp. 191-202 ◽  
Author(s):  
B�atrice Barraud ◽  
Sophie Balavoine ◽  
G�rard Feldmann ◽  
Bernard Lardeux
Keyword(s):  
Gene Expression ◽  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Acid Glycoprotein ◽  
Glycoprotein Gene ◽  
Normal Rat

scholarly journals ADD1/SREBP-1c Is Required in the Activation of Hepatic Lipogenic Gene Expression by Glucose

1999 ◽  
Vol 19 (5) ◽  
pp. 3760-3768 ◽  
Author(s):  
Marc Foretz ◽  
Corinne Pacot ◽  
Isabelle Dugail ◽  
Patricia Lemarchand ◽  
Colette Guichard ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fatty Acid Synthase ◽  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Dominant Negative ◽  
Temporal Association ◽  
Lipogenic Gene ◽  
Lipogenic Genes ◽  
Lipogenic Gene Expression ◽  
Carbohydrate Feeding

ABSTRACT The transcription of genes encoding proteins involved in the hepatic synthesis of lipids from glucose is strongly stimulated by carbohydrate feeding. It is now well established that in the liver, glucose is the main activator of the expression of this group of genes, with insulin having only a permissive role. While ADD1/SREBP-1 has been implicated in lipogenic gene expression through temporal association with food intake and ectopic gain-of-function experiments, no genetic evidence for a requirement for this factor in glucose-mediated gene expression has been established. We show here that the transcription of ADD1/SREBP-1c in primary cultures of hepatocytes is controlled positively by insulin and negatively by glucagon and cyclic AMP, establishing a link between this transcription factor and carbohydrate availability. Using adenovirus-mediated transfection of a powerful dominant negative form of ADD1/SREBP-1c in rat hepatocytes, we demonstrate that this factor is absolutely necessary for the stimulation by glucose of l-pyruvate kinase, fatty acid synthase, S14, and acetyl coenzyme A carboxylase gene expression. These results demonstrate that ADD1/SREBP-1c plays a crucial role in mediating the expression of lipogenic genes induced by glucose and insulin.

scholarly journals Synchronized synthesis and intracellular transport of serum albumin and apolipoprotein B in cultured rat hepatocytes as studied by double immunofluorescence.

1986 ◽  
Vol 34 (9) ◽  
pp. 1223-1230 ◽  
Author(s):  
G A Keller ◽  
C Glass ◽  
D Louvard ◽  
D Steinberg ◽  
S J Singer
Keyword(s):  
Serum Albumin ◽  
Apolipoprotein B ◽  
Intracellular Transport ◽  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Cell System ◽  
Secretory Proteins ◽  
Transport Pathway ◽  
Apo B ◽  
Normal Rat

Synthesis and intracellular transport of two secretory proteins, serum albumin (SA) and apolipoprotein B (apo B) have been synchronized in primary cultures of normal rat hepatocytes to make possible immunocytochemical study of the transport pathway. Under appropriate conditions of cycloheximide treatment, synthesis of new protein was inhibited and, by double immunofluorescent labeling, the cells were found to be largely depleted of the SA and apo B previously synthesized. Re-initiation of protein synthesis led to sequential appearance of SA and apo B, first in the endoplasmic reticulum, then in the Golgi complex, and finally at the cell surface. These results indicate that it should be feasible to use this cell system for high-resolution investigation of the sequence of structures involved in intracellular transport of SA and apo B by corresponding immunolabeling experiments as observed by electron microscopy.

scholarly journals Different effects of the glucosidase inhibitors 1-deoxynojirimycin, N-methyl-1-deoxynojirimycin and castanospermine on the glycosylation of rat α1-proteinase inhibitor and α1-acid glycoprotein

1986 ◽  
Vol 236 (3) ◽  
pp. 853-860 ◽  
Author(s):  
V Gross ◽  
T A Tran-Thi ◽  
R T Schwarz ◽  
A D Elbein ◽  
K Decker ◽  
...  
Keyword(s):  
Proteinase Inhibitor ◽  
De Novo ◽  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Complex Type ◽  
Glucose Residue ◽  
Acid Glycoprotein ◽  
Glucosidase Inhibitors ◽  
Oligosaccharide Processing ◽  
High Mannose

The glucosidase inhibitors 1-deoxynojirimycin, N-methyl-1-deoxynojirimycin and castanospermine were used to inhibit oligosaccharide processing in primary cultures of rat hepatocytes. Their effect on the glycosylation of alpha 1-proteinase inhibitor (alpha 1PI) and alpha 1-acid glycoprotein (alpha 1AGP) was studied. Of the three glucosidase inhibitors examined, 1-deoxynojirimycin inhibited not only oligosaccharide trimming but also glycosylation de novo of newly synthesized proteins, resulting in the formation of alpha 1PI with two and three (normally carrying three) and alpha 1AGP with two to five (normally carrying six) oligosaccharide side chains. In the presence of the glucosidase inhibitors, glucosylated high-mannose-type oligosaccharides accumulated. Whereas most of the endoglucosaminidase-H-sensitive oligosaccharides formed in the presence of 1-deoxynojirimycin contained only one glucose residue, N-methyl-1-deoxynojirimycin and castanospermine led mainly to the formation of oligosaccharides with three glucose residues. None of the three glucosidase inhibitors completely prevented the formation of complex-type oligosaccharides. Thus, in their presence, alpha 1PI and alpha 1AGP with a mixture of both high-mannose and complex-type oligosaccharides were secreted.

scholarly journals Collagen measured in primary cultures of normal rat hepatocytes derives from lipocytes within the monolayer.

1988 ◽  
Vol 82 (2) ◽  
pp. 450-459 ◽  
Author(s):  
J J Maher ◽  
D M Bissell ◽  
S L Friedman ◽  
F J Roll
Keyword(s):  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Normal Rat
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