Application of bradykinin radioimmunoassay for the measurement of urinary kallikrein activity in rats

1978 ◽  
Vol 56 (S1) ◽  
pp. 127-129 ◽  
Author(s):  
M. Stocker ◽  
J. Hornung
Keyword(s):  
Urinary Kallikrein ◽  
Kallikrein Activity

The Relationship of Urinary Kallikrein Activity to Renal Salt and Water Excretion

1978 ◽  
Vol 54 (1) ◽  
pp. 39-45 ◽  
Author(s):  
S. B. Levy ◽  
R. P. Frigon ◽  
R. A. Stone
Keyword(s):  
Racial Differences ◽  
Sodium Intake ◽  
Dietary Sodium ◽  
Racial Groups ◽  
Urinary Kallikrein ◽  
Experimental Conditions ◽  
Water Excretion ◽  
Water Load ◽  
Black And White ◽  
Kallikrein Activity

1. We measured urinary kallikrein (kininogenin) excretion in black and white normotensive subjects during a variety of manipulations of salt and water balance. 2. A large intravenous saline load administered while the subjects were on an unrestricted sodium diet did not significantly change urinary kallikrein activity in either racial group. 3. After several days of dietary sodium restriction both racial groups increased their urinary kallikrein activity. An intravenous water load given then further increased urinary kallikrein activity. White subjects were studied for an additional 24 h period, and urinary kallikrein activity returned to pre-water load values, indicating that the excretion of a water load in sodium-depleted subjects is associated with an increase in kallikrein excretion. 4. Black subjects excreted less kallikrein in the urine than white subjects during the initial 24 h periods of unrestricted dietary sodium intake, but there were no other significant racial differences during the other experimental conditions.

scholarly journals Measurement of urinary kallikrein activity by kinin radioimmunoassay.

1979 ◽  
Vol 43 (3) ◽  
pp. 147-152 ◽  
Author(s):  
KAZUAKI SHIMAMOTO ◽  
SHIGEMICHI TANAKA ◽  
TAKASHI NAKAO ◽  
TOSHIAKI ANDO ◽  
YASUYUKI NAKAHASHI ◽  
...  
Keyword(s):  
Urinary Kallikrein ◽  
Kallikrein Activity

Urinary kallikrein activity is increased during the first few weeks of exercise training in essential hypertension

1994 ◽  
Vol 12 (7) ◽  
pp. 815???824 ◽  
Author(s):  
Shin-ichiro Miura ◽  
Eiichiro Tashiro ◽  
Takaaki Sakai ◽  
Manabu Koga ◽  
Akio Kinoshita ◽  
...  
Keyword(s):  
Essential Hypertension ◽  
Exercise Training ◽  
Urinary Kallikrein ◽  
Kallikrein Activity

Changes in Renal and Urinary Kallikrein Activity by Mannitol-Induced Osmotic Diuresis

1981 ◽  
Vol 61 (1) ◽  
pp. 47-51 ◽  
Author(s):  
G. Bönner ◽  
M. Marin-Grez ◽  
D. Beck ◽  
M. Deeg ◽  
F. Gross
Keyword(s):  
Renal Cortex ◽  
Plasma Aldosterone ◽  
Urinary Kallikrein ◽  
Osmotic Diuresis ◽  
Plasma Aldosterone Concentration ◽  
Urinary Kallikrein Excretion ◽  
Short Period ◽  
Urinary Potassium ◽  
Renal Kallikrein ◽  
Kallikrein Activity

1. Osmotic diuresis was induced in male Sprague-Dawley rats by a 30% (w/v) mannitol solution injected three times at 15-min intervals. Kallikrein excretion increased for a short period after the first two injections, but, despite marked diuresis, the increment of kallikrein excretion after the second injection was less marked than after the first and no enhanced kallikrein excretion was observed after the third injection of mannitol. 2. Urinary kallikrein excretion correlated only with urinary potassium excretion. No correlation was found with either urine volume or urinary sodium excretion. 3. At the end of the osmotic diuresis kallikrein activity was significantly reduced both in the urine and in the renal cortex. At that time plasma aldosterone concentration was slightly greater in the mannitol-treated than that in the control group, but the difference did not reach statistical significance. 4. In this experiment no relationship was observed between the activity of the renal kallikrein-kinin system and the plasma aldosterone concentration. 5. The transient increase in urinary kallikrein excretion is interpreted as a wash-out effect of renal kallikrein, which is followed by a diminished kallikrein activity in urine and in renal cortex.

Reduced urinary kallikrein activity in rats developing spontaneous hypertension

1987 ◽  
Vol 252 (6) ◽  
pp. F964-F969 ◽  
Author(s):  
J. L. Ader ◽  
T. Tran-Van ◽  
F. Praddaude
Keyword(s):  
Urinary Excretion ◽  
Renal Excretion ◽  
Systolic Arterial Pressure ◽  
Sodium Balance ◽  
Urinary Kallikrein ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Wistar Kyoto ◽  
Kallikrein Activity ◽  
Lower Urinary

Urinary kallikrein activity, intake, and excretion of sodium and water were measured in metabolic balance studies on spontaneously hypertensive rats (SHR) of the Okamoto-Aoki strain and normotensive Wistar-Kyoto rats (WKY) on a normal sodium diet from 4 through 15 wk of age. Urinary excretion of active and total kallikrein was significantly lower in SHR at all ages examined and represented a stable percentage of the values in age-matched WKY throughout development, on the average 69.5 and 67.4%, respectively. SHR exhibited a lower urinary excretion of sodium and water than WKY, a higher cumulative sodium balance at all ages studied, and a higher cumulative water balance only at ages 7 and 8 wk. The slopes of the regression lines correlating urinary kallikrein to systolic arterial pressure and to urinary excretion and cumulative balance of sodium and water were always significantly less in SHR than in WKY. The results of the present study indicate that SHR developing hypertension exhibit a precocious and stable abnormality in renal excretion of kallikrein activity.

Dissociation of urinary kallikrein activity and salt and water excretion in the rat

1986 ◽  
Vol 250 (6) ◽  
pp. F1082-F1089 ◽  
Author(s):  
D. M. Pollock ◽  
M. I. Butterfield ◽  
J. L. Ader ◽  
W. J. Arendshorst
Keyword(s):  
Sodium Excretion ◽  
Linear Regression Analysis ◽  
Urine Flow ◽  
Urinary Kallikrein ◽  
Water Excretion ◽  
Time Control ◽  
Dose Dependent Decrease ◽  
State Group ◽  
Kallikrein Activity ◽  
The Relationship

Experiments were designed to examine the relationship, if any, between urinary kallikrein activity (amidolytic assay) and sodium and water excretion in 12-wk-old Munich-Wistar rats. Five groups of animals were studied: euvolemic, saline-expanded and water-loaded anesthetized rats, and euvolemic and saline-expanded conscious restrained rats. Following surgery, animals were allowed to stabilize (60-180 min) and reach a steady-state urine flow. By design, basal sodium and/or water excretion varied markedly among groups as a function of hydration state. Group means for sodium excretion and urine flow ranged from 0.8 to 12.4 mu eq/min and 6 to 112 microliter/min, respectively. In contrast, neither active nor total urinary kallikrein activity differed significantly among the five groups. In anesthetized euvolemic rats, intravenous administration of aprotinin produced a dose-dependent decrease in urinary kallikrein activity. The greatest inhibition of 93 +/- 3% (active) and 72 +/- 10% (total) was observed with a dose of 5,000 kallikrein inhibiting units (KIU)/kg and 1,000 KIU X kg-1 X min-1. This dose produced a significant decrease in active and total kallikrein activity in each group (P less than 0.001). However, sodium and water excretion were unchanged in aprotinin-treated rats and similar to values in vehicle-treated time-control groups. Linear regression analysis revealed no significant correlations between urinary kallikrein activity and sodium excretion or urine flow either among or within groups. These results indicate that urinary kallikrein activity is not related to acute sodium and water homeostasis in anesthetized or conscious rats.

Sign in / Sign up

Export Citation Format

Share Document