Ultrastructural immunocytochemical localization of B-50/GAP43, a protein kinase C substrate, in isolated presynaptic nerve terminals and neuronal growth cones

1989 ◽  
Vol 18 (4) ◽  
pp. 479-489 ◽  
Author(s):  
M. Van Lookeren Campagne ◽  
A. Beate Oestreicher ◽  
P. M. P. Van Bergen En Henegouwen ◽  
W. H. Gispen
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Growth Cones ◽  
Nerve Terminals ◽  
Immunocytochemical Localization ◽  
Neuronal Growth ◽  
Kinase C ◽  
Presynaptic Nerve Terminals ◽  
Neuronal Growth Cones

scholarly journals Protein kinase C activation decreases peripheral actin network density and increases central nonmuscle myosin II contractility in neuronal growth cones

2013 ◽  
Vol 24 (19) ◽  
pp. 3097-3114 ◽  
Author(s):  
Qing Yang ◽  
Xiao-Feng Zhang ◽  
David Van Goor ◽  
Ashleigh P. Dunn ◽  
Callen Hyland ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Myosin Ii ◽  
Growth Cones ◽  
Network Density ◽  
Actin Network ◽  
Neuronal Growth ◽  
Kinase C ◽  
Neuronal Growth Cones ◽  
Pkc Activation

Protein kinase C (PKC) can dramatically alter cell structure and motility via effects on actin filament networks. In neurons, PKC activation has been implicated in repulsive guidance responses and inhibition of axon regeneration; however, the cytoskeletal mechanisms underlying these effects are not well understood. Here we investigate the acute effects of PKC activation on actin network structure and dynamics in large Aplysia neuronal growth cones. We provide evidence of a novel two-tiered mechanism of PKC action: 1) PKC activity enhances myosin II regulatory light chain phosphorylation and C-kinase–potentiated protein phosphatase inhibitor phosphorylation. These effects are correlated with increased contractility in the central cytoplasmic domain. 2) PKC activation results in significant reduction of P-domain actin network density accompanied by Arp2/3 complex delocalization from the leading edge and increased rates of retrograde actin network flow. Our results show that PKC activation strongly affects both actin polymerization and myosin II contractility. This synergistic mode of action is relevant to understanding the pleiotropic reported effects of PKC on neuronal growth and regeneration.

scholarly journals Neurogranin: immunocytochemical localization of a brain-specific protein kinase C substrate

1990 ◽  
Vol 10 (12) ◽  
pp. 3782-3792 ◽  
Author(s):  
A Represa ◽  
JC Deloulme ◽  
M Sensenbrenner ◽  
Y Ben-Ari ◽  
J Baudier
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Specific Protein ◽  
Immunocytochemical Localization ◽  
Kinase C ◽  
Specific Protein Kinase

scholarly journals Immunocytochemical localization of protein kinase C isozymes in rat brain

1988 ◽  
Vol 8 (12) ◽  
pp. 4734-4744 ◽  
Author(s):  
FL Huang ◽  
Y Yoshida ◽  
H Nakabayashi ◽  
WS Young ◽  
KP Huang
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Rat Brain ◽  
Immunocytochemical Localization ◽  
Kinase C
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