The formation of heinz bodies in ghosts of human erythrocytes of adults and newborn infants

1973 ◽  
Vol 51 (4) ◽  
pp. 201-203 ◽  
Author(s):  
W. Tillmann ◽  
J. Menke ◽  
W. Schr�ter
Keyword(s):  
Newborn Infants ◽  
Human Erythrocytes ◽  
Heinz Bodies

Heinz-Body Anaemia in the Newborn

PEDIATRICS ◽  
1957 ◽  
Vol 19 (6) ◽  
pp. 1001-1001
Keyword(s):  
Hemolytic Anemia ◽  
Case Reports ◽  
Newborn Infants ◽  
Term Infant ◽  
Heinz Bodies ◽  
Heinz Body ◽  
Large Numbers ◽  
Complete Reversal ◽  
Staining Techniques ◽  
Supravital Staining

The association of Heinz-bodies within erythrocytes, extreme distortion of the size and shape of erythrocytes and hemolytic anemia in newborn infants, especially premature infants, has been sporadically reported in the medical literature since 1948. Heinz-bodies are thought to be either remnants of disintegrated membranes of erythocytes or abnormal products of hemoglobin metablism, and are demonstrable only by supravital staining techniques. The present study contributes two case reports and discusses the sequence of the clinical and hematologic manifestations. The first patient was a full-term infant who developed jaundice and symptoms attributable to anemia at 2 weeks of life. The second infant was prematurely born, did not develop jaundice but showed large numbers of Heinz-bodies (70%) as the anemia progressed. Both patients responded well to a single transfusion of packed blood cells, with complete reversal of the abnormal peripheral blood findings. None of the usual causes of hemolysis could be demonstrated by extensive laboratory tests. Agents such as phenylhydrazine, known to produce Heinz-bodies, could not be incriminated. The phenomenon of Heinz-body formation in infants may be more common than is apparent as the technique of demonstration is not commonly a part of the routine study of infants with evidence of hemolytic anemia. This technique is described and illustrations of erythrocytes containing Heinz-bodies are provided.

Prevention of Ozonide-Induced Heinz Bodies in Human Erythrocytes by Vitamin E

1975 ◽  
Vol 30 (5) ◽  
pp. 234-236 ◽  
Author(s):  
Daniel B. Menzel ◽  
Ronald J. Slaughter ◽  
Anita M. Bryant ◽  
Hugo O. Jauregui
Keyword(s):  
Vitamin E ◽  
Human Erythrocytes ◽  
Heinz Bodies

scholarly journals Heinz bodies do not modify the membrane characteristics of common marmoset (Callithrix jacchus) erythrocytes

1989 ◽  
Vol 23 (1) ◽  
pp. 66-69 ◽  
Author(s):  
S. C. Omorphos ◽  
C. Rice-Evans ◽  
C. Hawkey
Keyword(s):  
Surface Charge ◽  
Reduced Glutathione ◽  
Common Marmoset ◽  
Callithrix Jacchus ◽  
Human Erythrocytes ◽  
Membrane Function ◽  
Common Marmosets ◽  
Heinz Bodies ◽  
Normal Human ◽  
Malonyl Dialdehyde

The possibility was examined that the membrane function of erythrocytes obtained from healthy common marmosets ( Callithrix jacchus) was modified by the presence in the cells of Heinz bodies. No significant differences were found in erythrocyte endogenous free malonyl dialdehyde (MDA) or reduced glutathione (GSH) between normal human erythrocytes and marmoset erythrocytes containing Heinz bodies. Membrane fluorescent chromolipids, surface charge and thiol levels were similar in both species but average membrane bulk lipid fluidity was slightly elevated in the marmosets. It was concluded that, in contrast to the situation in human erythrocytes, the presence of Heinz bodies in red cells of marmosets does not adversely affect the properties of the membrane.

Elemental analysis of human erythrocytes

1989 ◽  
Vol 47 ◽  
pp. 242-243
Author(s):  
S. A. Livesey ◽  
A. A. del Campo ◽  
E. S. Griffey ◽  
D. Ohlmer ◽  
T. Schifani ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Elemental Analysis ◽  
Human Erythrocyte ◽  
Spectroscopic Analysis ◽  
Model System ◽  
Human Erythrocytes ◽  
List Type ◽  
Chemical Fixation ◽  
Ethanol Dehydration ◽  
Lowicryl K4m

The aim of this study is to compare methods of sample preparation for elemental analysis. The model system which is used is the human erythrocyte. Energy dispersive spectroscopic analysis has been previously reported for cryofixed and cryosectioned erythrocytes. Such work represents the reference point for this study. The use of plastic embedded samples for elemental analysis has also been documented. The work which is presented here is based on human erythrocytes which have been either chemically fixed and embedded or cryofixed and subsequently processed by a variety of techniques which culminated in plastic embedded samples.Heparinized and washed erythrocytes were prepared by the following methods for this study :(1). Chemical fixation in 4% paraformaldehyde/0.25% glutaraldehyde/0.2 M sucrose in 0.1 M Na cacodylate, pH 7.3 for 30 min, followed by ethanol dehydration, infiltration and embedding in Lowicryl K4M at -20° C.

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