Two-dimensional protein electrophoretic analysis of postponed aging inDrosophila

Genetica ◽  
1993 ◽  
Vol 91 (1-3) ◽  
pp. 183-198 ◽  
Author(s):  
James E. Fleming ◽  
Greg S. Spicer ◽  
Roger C. Garrison ◽  
Michael R. Rose
Keyword(s):  
Electrophoretic Analysis ◽  
Two Dimensional ◽  
Postponed Aging

Two-dimensional protein electrophoretic analysis of postponed aging in Drosophila

2004 ◽  
pp. 205-220
Author(s):  
James E. Fleming ◽  
Greg S. Spicer ◽  
Roger C. Garrison ◽  
Michael R. Rose
Keyword(s):  
Electrophoretic Analysis ◽  
Two Dimensional ◽  
Postponed Aging

Two-dimensional electrophoretic analysis of cytosols from human breast tumors: optimal migration conditions.

1984 ◽  
Vol 30 (12) ◽  
pp. 1947-1949 ◽  
Author(s):  
P Motté ◽  
J M Bidart ◽  
J C Delarue ◽  
E Comoy ◽  
P Moingeon ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Breast Tumors ◽  
Electrophoretic Analysis ◽  
Ph Gradient ◽  
Two Dimensional ◽  
Human Breast ◽  
Cytosolic Proteins ◽  
Optimal Migration ◽  
Dodecyl Sulfate

Abstract In an examination of cytosols from human breast tumors, we performed two-dimensional electrophoretic analysis. Several migration conditions were tried in a search for a homogeneous repartition of cytosolic proteins. The most workable gels were obtained with a 4.40 to 8.05 pH gradient in the first dimension and a homogeneous 125 g/L acrylamide/sodium dodecyl sulfate gel, 1 mm in thickness, in the second dimension. The practicability of this method is discussed.

Head-down tilt increases rat cardiac muscle eIF-2 alpha phosphorylation

1995 ◽  
Vol 269 (3) ◽  
pp. C802-C804 ◽  
Author(s):  
V. Menon ◽  
D. B. Thomason
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein 70 ◽  
Center Of Mass ◽  
Electrophoretic Analysis ◽  
Initiation Factor ◽  
Two Dimensional ◽  
Eukaryotic Initiation Factor ◽  
Hsp 70 ◽  
Initiation Rate ◽  
Initiation Factor 2

We previously demonstrated that head-down tilt in rats decreases heart polypeptide initiation rate and proposed a mechanism whereby redistribution of the chaperone heat-shock cognate/heat-shock protein-70 (HSC/HSP-70) facilitates the phosphorylation of eukaryotic initiation factor-2 alpha (eIF-2 alpha). In this study, two-dimensional gel electrophoretic analysis of eIF-2 alpha showed no phosphorylation in control hearts. At 8 h of head-down tilt, there was a 45% increase in total eIF-2 alpha, and 79% was phosphorylated. At 18 h, eIF-2 alpha increased to 142% of control, of which 4% was phosphorylated. This is consistent with the previous study where, at 8 h, there was a 78% increase in polysomal HSC/HSP-70 and a shift in the polysome center-of-mass to lighter polysomes (indicating decreased initiation). After 18 h of suspension, polysomal HSC/HSP-70 levels were 24% relative to control, and the center-of-mass returned toward control. We conclude that the decrease in polypeptide initiation during head-down tilt is mediated by HSC/HSP-70 via phosphorylation of eIF-2 alpha.

Procedures for two-dimensional electrophoretic analysis of nuclear proteins

1986 ◽  
Vol 351 ◽  
pp. 77-89 ◽  
Author(s):  
Thierry Rabilloud ◽  
Michelle Hubert ◽  
Philippe Tarroux
Keyword(s):  
Electrophoretic Analysis ◽  
Nuclear Proteins ◽  
Two Dimensional
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