The sequential change of local cerebral blood flow and local cerebral glucose metabolism after focal cerebral ischaemia and reperfusion in rat and the effect of MK-801 on local cerebral glucose metabolism

1997 ◽  
Vol 139 (8) ◽  
pp. 770-779 ◽  
Author(s):  
Yunhui Liu ◽  
S. Mituska ◽  
K. Hashizume ◽  
T. Hosaka ◽  
H. Nukui
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Glucose Metabolism ◽  
Cerebral Ischaemia ◽  
Cerebral Glucose Metabolism ◽  
Focal Cerebral Ischaemia ◽  
Local Cerebral Blood Flow ◽  
Mk 801 ◽  
Sequential Change ◽  
Ischaemia And Reperfusion

scholarly journals Perivascular Microapplication of Endothelin-1: A New Model of Focal Cerebral Ischaemia in the Rat

1993 ◽  
Vol 13 (5) ◽  
pp. 865-871 ◽  
Author(s):  
John Sharkey
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Cerebral Ischaemia ◽  
Dorsal Hippocampus ◽  
Focal Cerebral Ischaemia ◽  
Local Cerebral Blood Flow ◽  
Guide Cannula ◽  
Conscious Rat ◽  
Endothelin 1 ◽  
Arterial Blood

In the present study, we describe the effects of perivascular microapplication of the potent vasoconstrictor peptide endothelin-1 (Et-1; (120 pmol in 3 μl), delivered via a guide cannula stereotaxically positioned above the left cerebral artery (MCA) of the conscious male Sprague–Dawley rat. Ten minutes after the administration of Et-1, mean arterial blood pressure had increased by 20% and profound reductions in local cerebral blood flow (up to 93%) were observed within those brain areas supplied by the MCA. In addition, significant increases in local cerebral blood flow were observed within the globus pallidus (100%), substantia nigra pars reticulata (48%), ventrolateral thalamus (65%), and dorsal hippocampus (74%) ipsilateral to the insult. Twenty-four hours following the insult, the pattern of ischaemic damage was similar to that reported previously following permanent occlusion of the rat MCA. It is suggested that perivascular microapplication of Et-1 may provide a useful model for the study of the functional disturbances associated with focal cerebral ischaemia in the conscious rat.

scholarly journals Evaluation of a Double-Tracer Autoradiographic Technique for the Measurement of Both Local Cerebral Glucose Metabolism and Local Cerebral Blood Flow

1985 ◽  
Vol 5 (2) ◽  
pp. 335-337 ◽  
Author(s):  
Stephen C. Jones ◽  
Joel H. Greenberg
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Glucose Metabolism ◽  
Brain Tissue ◽  
Simultaneous Measurement ◽  
Cerebral Glucose Metabolism ◽  
Careful Attention ◽  
Local Cerebral Blood Flow ◽  
Tissue Sections ◽  
Autoradiographic Technique

A solvent washout technique is evaluated that could be used to remove one 14C tracer after a combined autoradiographic CMRglu and CBF study using [14C]2-deoxyglucose (2DG) and [14C]iodoantipyrine (IAP). The technique permits the simultaneous measurement of local CBF and local CMRglu in the same animal without the use of the short-lived tracers of iodine, 123I and 131I, for iodoantipyrine labeling. This report shows that brain tissue sections did not retain 14C from [14C]2DG when chloroform was used as the washout solvent. Chloroform washing removes nearly all the 14C from [14C]IAP. For this study, careful attention was given to the control, pre-wash measurement of 14C concentration.

Colloidal volume expansion during acute cerebral ischaemia: assessed by local cerebral blood flow and computerized power ratio index

1988 ◽  
Vol 10 (3) ◽  
pp. 151-155 ◽  
Author(s):  
Bruce I. Tranmer ◽  
Cordell E. Gross ◽  
Geoff R. Adey ◽  
Ted S. Keller ◽  
Ken Nagata ◽  
...  
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Cerebral Ischaemia ◽  
Volume Expansion ◽  
Power Ratio ◽  
Local Cerebral Blood Flow ◽  
Acute Cerebral Ischaemia

Reduction of Edema and Infarction by Memantine and MK-801 After Focal Cerebral Ischaemia and Reperfusion in Rat

2000 ◽  
Vol 142 (11) ◽  
pp. 1287-1292 ◽  
Author(s):  
A. Görgülü ◽  
T. Kınş ◽  
S. Çobanoğlu ◽  
F. U¨nal ◽  
N. İ zgi ◽  
...  
Keyword(s):  
Cerebral Ischaemia ◽  
Focal Cerebral Ischaemia ◽  
Mk 801 ◽  
Ischaemia And Reperfusion

scholarly journals Influence of early posttraumatic hypothermia therapy on local cerebral blood flow and glucose metabolism after fluid-percussion brain injury

2001 ◽  
Vol 11 (4) ◽  
pp. 510-519
Author(s):  
Weizhao Zhao ◽  
Ofelia F. Alonso ◽  
Judith Y. Loor ◽  
Raul Busto ◽  
Myron D. Ginsberg
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Glucose Metabolism ◽  
Glucose Utilization ◽  
Linear Regression Analysis ◽  
Data Sets ◽  
Imaging Data ◽  
Local Cerebral Blood Flow ◽  
Fluid Percussion ◽  
The Right

Object Using autoradiographic image averaging, the authors recently described prominent foci of marked glucose metabolism-greater-than-blood-flow uncoupling in the acutely traumatized rat brain. Because hypothermia is known to ameliorate injury in this and other injury models, the authors designed the present study to assess the effects of post-traumatic therapeutic hypothermia on the local cerebral metabolic rate of glucose (LCMRglu) and local cerebral blood flow (LCBF) following moderate parasagittal fluid-percussion head injury (FPI) in rats. Methods Either cranial hypothermia (30°C) or normothermia (37°C) was induced for 3 hours in matched groups of rats immediately after FPI; LCMRglu and LCBF were assessed 3 hours after concluding these temperature manipulations. In rats subjected to FPI, regardless of whether normothermia or hypothermia ensued, LCBF was reduced relative to the sham-injury groups. In addition, when FPI was followed by hypothermia (FPI–30°C group), the subsequent LCBF was significantly lower (35–38% on average) than in FPI–37°C rats. Statistical mapping of LCBF difference imaging data revealed confluent cortical and subcortical zones of significantly reduced LCBF (largely ipsilateral to the prior injury) in FPI–30°C rats relative to the FPI–37°C group. Local glucose utilization was reduced in both hemispheres of FPI–37°C rats relative to the sham-injury group and was lower in the right (traumatized) hemisphere than in the left. However, LCMRglu values were largely unaffected by temperature manipulation in either the FPI or sham-injury groups. The LCMRglu/LCBF ratio was nearly doubled in FPI–30°C rats relative to the FPI–37°C group, in a diffuse and bihemispheric fashion. Linear regression analysis comparing LCMRglu and LCBF revealed that the FPI–37°C and FPI–30°C data sets were completely nonoverlapping, whereas the two sham-injury data sets were intermixed. Conclusions Despite its proven neuroprotective efficacy, early posttraumatic hypothermia (30°C for 3 hours) nonetheless induces a moderate decline in cerebral perfusion without the (anticipated) improvement in cerebral glucose utilization, so that a state of mild metabolism-greater-than-blood-flow dissociation is perpetuated.

Effects of an analogue of thyrotrophin-releasing hormone, RX77368, on infarct size and cerebral blood flow in focal cerebral ischaemia in the rat

1989 ◽  
Vol 67 (10) ◽  
pp. 1345-1350 ◽  
Author(s):  
C. T. O'Shaughnessy ◽  
N. J. Rothwell ◽  
J. Shrewsbury-Gee
Keyword(s):  
Blood Flow ◽  
Cerebral Blood Flow ◽  
Infarct Size ◽  
Middle Cerebral Artery ◽  
Cerebral Ischaemia ◽  
Lesion Size ◽  
Focal Cerebral Ischaemia ◽  
Thyrotrophin Releasing Hormone ◽  
Releasing Hormone ◽  
Stimulation Of

Effects of a stable analogue of thyrotrophin-releasing hormone, RX77368, on cerebral blood flow and infarct size have been studied in an acute model of cerebral ischaemia in the rat. Two hours after electrocoagulation of the left middle cerebral artery (MCA), the mean area of ischaemia (± SEM), determined histochemically, was 11.5 ± 2.2% of a single hemisphere and blood flow, determined using radiolabeled microspheres, was reduced by 40% in the left forebrain (p < 0.001 compared with sham-operated animals). Administration of RX77368 (50 μg/kg, intracerebroventricularly) within 10 min of arterial occlusion caused a significant (p < 0.01) reduction in mean lesion size to 3.7 ± 1.8% and stimulation of blood flow to the left ischaemic forebrain (60% above saline treated). Peripheral administration of RX77368 (1 mg/kg intraperitoneally) also significantly stimulated blood flow to the ischaemic forebrain and caused an apparent decrease in frequency of large infarcted areas of brain tissue, although mean lesion size was not significantly affected. These findings indicate that RX77368 ameliorates tissue damage in acute focal cerebral ischaemia. Such effects may be related to stimulation of cerebral blood flow.Key words: middle cerebral artery, focal cerebral ischaemia, cerebral blood flow, thyrotrophin-releasing hormone analogue.

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