Root contraction in hyacinth III. Orientation of cortical microtubules visualized by immunofluorescence microscopy

PROTOPLASMA ◽  
1989 ◽  
Vol 151 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Nancy L. Smith-Huerta ◽  
Judith A. Jernstedt
Keyword(s):  
Immunofluorescence Microscopy ◽  
Cortical Microtubules ◽  
Root Contraction

Computer-aided 3-D reconstruction of interphase epidermal cells of Datura stramonium reveals assembly

Development ◽  
1989 ◽  
Vol 106 (3) ◽  
pp. 531-541
Author(s):  
D.J. Flanders ◽  
D.J. Rawlins ◽  
P.J. Shaw ◽  
C.W. Lloyd
Keyword(s):  
Immunofluorescence Microscopy ◽  
Datura Stramonium ◽  
Epidermal Cells ◽  
Crossing Over ◽  
Cortical Microtubules ◽  
Cellulase Treatment ◽  
Cell Arrays ◽  
Side Walls ◽  
Polyhedral Cells ◽  
Set Up

From immunofluorescence microscopy it has been cortical microtubules form whole-cell arrays. This has clearly seen in cylindrical hairs where the existence testifies to the continuity of the array around the cell. It is not, however, clear how microtubules pack polyhedral cells with multiple, angled facets. In problem, elongated and isodiametric cells in the stramonium L. were subjected to anti-tubulin avoiding distortion by cellulase treatment and air- sections were then deblurred by computer, the digitized, reconstructed and then rotated in order to arrangement of microtubules along the anticlinal walls This established several things. Microtubules tend to any one cell face; they form transverse, oblique or except that some walls bear a crisscross arrangement. cells, microtubules clearly form helices. In the cells, transversely wound microtubules are confirmed continuous from one face to another and probably, constitute helices. Microtubules on oblique end walls continue onto the side walls and do not form a microtubules can be ordered upon two adjacent facets, with respect to the stem's axis need not necessarily both facets, i.e. overall alignment can change at the isodiametric epidermal cells, microtubules can one cell facet to another. However, where microtubules anticlinal walls spill over onto a periclinal wall at a crisscross arrangement is set up. This is attributed geometrical problem of fitting parallel lines around polyhedra. Despite crossing over one another, the walls are nevertheless continuous with MTs on the side conclusion, in elongated cells the arrays still various pitch: in isodiametric cells (where the walls non-orthogonal angles to one another) the integrity of appears to be preserved by microtubules crossing over what is termed a ‘sacrificial’ face. The overriding microtubules to form an integral array regardless of

The application of video-enhanced constrast/differential interference constrast microscopy in conjunction with whole mount electron microscopy to the study of organelle transport

1988 ◽  
Vol 46 ◽  
pp. 66-67
Author(s):  
J. H. Hayden
Keyword(s):  
Electron Microscopy ◽  
Rana Pipiens ◽  
Silver Film ◽  
Immunofluorescence Microscopy ◽  
Organelle Transport ◽  
Linear Element ◽  
Whole Mount ◽  
Carbon Coated ◽  
Linear Elements ◽  
Dic Microscopy

In a previous study, Allen video-enhanced constrast/differential interference constrast (AVEC-DIC) microscopy was used in conjunction with immunofluorescence microscopy to demonstrate that organelles and vesicle move in either direction along linear elements composed of microtubules. However, this study was limited in that the number of microtubules making up a linear element could not be determined. To overcome this limitation, we have used AVEC-DIC microscopy in conjunction with whole mount electron microscopy.Keratocytes from Rana pipiens were grown on glass coverslips as described elsewhere. Gold London Finder grids were Formvar- and carbon coated, and sterilized by exposure to ultraviolet light. It is important to select a Formvar film that gives a grey reflection when it is floated on water. A silver film is too thick and will detract from the image in the light microscope.

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