Production of a polar metabolite of 1,25-dihydroxyvitamin D3 in a rat liver perfusion system

1981 ◽  
Vol 26 (3) ◽  
pp. 242-246 ◽  
Author(s):  
Rajiv Kumar ◽  
Sreeramulu Nagubandi ◽  
James M. Londowski
Keyword(s):  
Rat Liver ◽  
Liver Perfusion ◽  
Perfusion System ◽  
Dihydroxyvitamin D3 ◽  
Polar Metabolite ◽  
Rat Liver Perfusion ◽  
1,25 Dihydroxyvitamin D3

How to measure first-order hepatic transfer coefficients by distributed modeling of a recirculating rat liver perfusion system

1982 ◽  
Vol 243 (6) ◽  
pp. G518-G531
Author(s):  
B. A. Luxon ◽  
P. D. King ◽  
E. L. Forker
Keyword(s):  
Rat Liver ◽  
Liver Perfusion ◽  
Normal Liver ◽  
Perfusion System ◽  
Transfer Coefficients ◽  
Flow Measurements ◽  
Distributed Modeling ◽  
First Order ◽  
Rat Liver Perfusion ◽  
Transit Times

A digital computer is used to simulate solute disappearance curves evolving in the reservoir of a rat liver perfusion system. The results show that it is feasible to obtain good estimates of these kinetic parameters with only approximate information about the distribution of sinusoidal flows and the transfer function of the nonexchanging vasculature. By contrast satisfactory estimates of the transfer coefficients for uptake and efflux (but not excretion) are critically dependent on accurate measurements of the system transit times. A perfusion system suitable for implementing the method is tested and found to maintain normal liver function for upward of 60 min. The means to achieve the required volume and flow measurements are discussed, and an empirical criterion is developed for recognizing disappearance curves whose information content is too low to yield reliable results.

EFFECTS OF INSULIN AND GLUCAGON ON PRODUCTION OF RENIN SUBSTRATE BY THE ISOLATED RAT LIVER

1980 ◽  
Vol 85 (1) ◽  
pp. 151-153 ◽  
Author(s):  
EIKI MURAKAMI ◽  
KUNIO HIWADA ◽  
TATSUO KOKUBU
Keyword(s):  
Normal Control ◽  
Rat Liver ◽  
Liver Perfusion ◽  
Perfusion System ◽  
Angiotensin I ◽  
Renin Substrate ◽  
Isolated Rat Liver ◽  
Rat Liver Perfusion ◽  
Isolated Rat

SUMMARY An isolated rat liver perfusion system was used to study the effects of insulin and glucagon on renin substrate production. Normal livers synthesized renin substrate at a rate of 28·3 ± 3·8 (s.e.m.) ng angiotensin I equiv./g liver each h (n = 8). The addition of insulin (more than 0·1 i.u.) to the perfusion significantly enhanced the production of renin substrate which was about twofold higher than normal control values (P< 0·001). However, glucagon (20 μg) did not affect the synthesis of renin substrate. These results indicated that insulin promoted the synthesis of renin substrate by the isolated rat liver.

scholarly journals Evaluation for effect of hypothermia on the disposition of 4-nitrophenol in rats by in-vitro metabolism study and rat liver perfusion system

2013 ◽  
Vol 65 (10) ◽  
pp. 1536-1540 ◽  
Author(s):  
Hirotaka Miyamoto ◽  
Satoshi Matsueda ◽  
Kotaro Komori ◽  
Shintaro Fumoto ◽  
Mikiro Nakashima ◽  
...  
Keyword(s):  
Rat Liver ◽  
Liver Perfusion ◽  
In Vitro Metabolism ◽  
Perfusion System ◽  
Rat Liver Perfusion ◽  
Metabolism Study

Uptake Characteristics of Oligonucleotides in the Isolated Rat Liver Perfusion System

1996 ◽  
Vol 6 (3) ◽  
pp. 177-183 ◽  
Author(s):  
YOSHINOBU TAKAKURA ◽  
RAM I. MAHATO ◽  
MITSUNOBU YOSHIDA ◽  
TARO KANAMARU ◽  
MITSURU HASHIDA
Keyword(s):  
Rat Liver ◽  
Liver Perfusion ◽  
Perfusion System ◽  
Isolated Rat Liver ◽  
Rat Liver Perfusion ◽  
Isolated Rat
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