Studies on mixed fungal culture for cellulase and hemi-cellulase production part-1: Optimization of medium for the mixed culture ofTrichoderma reesei D1-6 andAspergillus Pt 2804

1983 ◽  
Vol 5 (11) ◽  
pp. 767-772 ◽  
Author(s):  
T. Panda ◽  
V. S. Bisaria ◽  
T. K. Ghose
Keyword(s):  
Mixed Culture ◽  
Cellulase Production ◽  
Fungal Culture ◽  
Mixed Fungal Culture ◽  
Production Part

Enzyme production by the mixed fungal culture with nano-shear pretreated biomass and lignocellulose hydrolysis

2013 ◽  
Vol 110 (8) ◽  
pp. 2123-2130 ◽  
Author(s):  
Jue Lu ◽  
Rankothge Ranjith Weerasiri ◽  
Yan Liu ◽  
Wei Wang ◽  
Shaowen Ji ◽  
...  
Keyword(s):  
Enzyme Production ◽  
Fungal Culture ◽  
Mixed Fungal Culture ◽  
Lignocellulose Hydrolysis ◽  
Pretreated Biomass

Isolation of Human Cancer Cell Growth Inhibitory, Antimicrobial Lateritin from a Mixed Fungal Culture

Planta Medica ◽  
2009 ◽  
Vol 76 (05) ◽  
pp. 500-501 ◽  
Author(s):  
Robin Pettit ◽  
George Pettit ◽  
Jung-Ping Xu ◽  
Christine Weber ◽  
Linda Richert
Keyword(s):  
Cell Growth ◽  
Cancer Cell ◽  
Human Cancer ◽  
Fungal Culture ◽  
Human Cancer Cell ◽  
Cancer Cell Growth ◽  
Growth Inhibitory ◽  
Mixed Fungal Culture

scholarly journals Culturable fungal diversity and cellulase production by mixed culture Aspergillus fungi from Sanya mangrove

2018 ◽  
Vol 64 (6) ◽  
pp. 293-298
Author(s):  
Siquan Zeng ◽  
Juan Ling ◽  
Manzoor Ahmad ◽  
Liyun Lin ◽  
Yanying Zhang ◽  
...  
Keyword(s):  
Mixed Culture ◽  
Fungal Diversity ◽  
Cellulase Production

Bioremediation of cardboard recycling industry effluents using mixed fungal culture

2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Zeinab Hosseini ◽  
Mohammad Taghi Ghaneian ◽  
Mahin Ghafourzade ◽  
Abbasali Jafari Nodoushan
Keyword(s):  
Removal Efficiency ◽  
Fungal Spores ◽  
Cod Removal ◽  
Fungal Culture ◽  
Potato Dextrose Agar Medium ◽  
Content Type ◽  
Recycling Industry ◽  
Cod Removal Efficiency ◽  
Mixed Fungal Culture ◽  
The Mean

Purpose This paper aims to evaluate the bioremediation [chemical oxygen demand (COD) and color removal] of the effluent from the cardboard recycling industry in Yazd, central province of Iran, using mixed fungal culture. Design/methodology/approach First, the effluent samples from the cardboard recycling industry were cultured on potato dextrose agar medium to isolate native fungal colonies. The grown colonies were then identified using morphological macroscopic and microscopic characteristics to choose the dominant fungi for bioremediations. The mixed cultures of Aspergillus niger, Aspergillus flavus and Penicillium digitatum were finally used for bioremediation experiments of the cardboard recycling industry. A suspension containing 1 × 106 CFU/ml of fungal spores was prepared from each fungus, separately and their homogenous mixture. Sewage samples were prepared and sterilized and used at 25%, 50% and 90% dilutions and pH levels of 5, 7 and 8 for bioremediation tests using mixed fungal spores. Following that, 10 ml of the mixed fungal spores were inoculated into the samples for decolorization and COD removal and incubated for 10 days at 30°C. The amount of COD removal and decolorization were measured before incubation and after 3, 6 and 10 days of inoculation. In this research, the color was measured by American Dye Manufacturer Institute and COD by the closed reflux method. The results of the present study were analyzed using SPSS 21 statistical software and one-way ANOVA tests at p-value < 0.05. Findings The results of this research showed that the mean decolorization by mixed fungal culture over 10 days at pH levels of 5, 7 and 8 were 44.40%, 45.00% and 36.84%, respectively, and the mean COD removal efficiency was 71.59%, 73.54% and 16.55%, respectively. Moreover, the mean decolorization at dilutions of 25%, 50% and 90% were 45.00%, 31.93% and 30.53%, respectively, and the mean COD removal efficiency was 73.54%, 62.38% and 34.93%, respectively. Therefore, the maximal COD removal and decolorization efficiency was obtained at dilution of 25% and pH 7. Originality/value Given that limited studies have been conducted on bioremediation of the effluent from the cardboard recycling industry using fungal species, this research could provide useful information on the physicochemical properties of the effluent in this industry.

scholarly journals Microbial oil production byMortierella isabellinafrom corn stover under different pretreatments

RSC Advances ◽  
2017 ◽  
Vol 7 (89) ◽  
pp. 56239-56246 ◽  
Author(s):  
Chen Zhao ◽  
Lu Deng ◽  
Hao Fang ◽  
Shaolin Chen
Keyword(s):  
Enzymatic Hydrolysis ◽  
Aspergillus Niger ◽  
Mixed Culture ◽  
Corn Stover ◽  
Trichoderma Reesei ◽  
Oil Production ◽  
Cellulase Production ◽  
Microbial Oil ◽  
Pretreated Corn Stover ◽  
Hydrolysis Of

Mixed culture ofTrichoderma reeseiandAspergillus nigerwas employed to accomplish on-site cellulase production where cellulases were applied directly to the enzymatic hydrolysis of pretreated corn stover.

scholarly journals Production of cellulase from Aspergillus niger and Trichoderma reesei mixed culture in carboxymethylcellulose medium as sole carbon

2019 ◽  
Vol 20 (12) ◽  
Author(s):  
Dia Septiani ◽  
HERMAN SURYADI ◽  
Abdul Mun'im ◽  
WIBOWO MANGUNWARDOYO
Keyword(s):  
Aspergillus Niger ◽  
Ammonium Sulfate ◽  
Mixed Culture ◽  
Trichoderma Reesei ◽  
Crude Extract ◽  
Hydrolytic Enzymes ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Partial Purification ◽  
Cellulose Depolymerization

Abstract. Septiani D, Suryadi H, Mun’im A, Mangunwardoyo W. 2019. Production of cellulase from Aspergillus niger and Trichoderma reesei mixed culture in carboxymethylcellulose medium as sole carbon. Biodiversitas 20: 3539-3544. Cellulase is one of hydrolytic enzymes that breakdown cellulose into glucose. Cellulases are promising to be applied in natural products which may improve the yield of bioactive in plant extract through cellulose depolymerization. Cellulases from mixed culture of Aspergillus niger and Trichoderma reesei can produce a high cellulase activity because of the synergism activity among endoglucanase, exoglucanase, and also β-glucoside. Cellulase production and partial purification of monoculture and mixed culture (1:1) of these fungi on carboxymethylcellulose media were investigated in this study. Total cellulase activity was measured by filter paper assay followed by protein estimation with Bradford method. The crude extract of Aspergillus niger monoculture has the highest cellulase activity (0.131 U/mL, P<005) followed by mixed culture (0.109 U/mL) and Trichoderma reesei (0.106 U/mL). The cellulase activity of partially purified cellulase from mixed culture significantly increased (0.335, 0.348, 0.374 U/mL, P<0.05) compared to crude extract along with stepwise addition of ammonium sulfate. Cellulase activity of mixed culture at 80% ammonium sulfate increase up to 2.238-fold and showed highest value (P<0.05) compared to monocultures. In conclusion, combination of Aspergillus niger and Trichoderma reesei fungi in carboxymethyl cellulose media followed by 80% ammonium sulfate precipitation can be a promising cellulase production with high cellulase activity.

scholarly journals CHARACTERIZATION OF CRUDE CELLULASE OF SEAGRASS ENDOPHYTIC FUNGUS

2014 ◽  
Vol 6 (1) ◽  
Author(s):  
Yulia Oktavia ◽  
Aulia Andhikawati ◽  
Tati Nurhayati ◽  
Kustiariyah Tarman
Keyword(s):  
Endophytic Fungus ◽  
Cellulase Activity ◽  
Enzyme Characterization ◽  
Cellulase Production ◽  
Fungal Culture ◽  
Endoglucanase Activity ◽  
Maximum Reaction Rate ◽  
Glucosidase Activity ◽  
Maximum Reaction

<p>In this study, cellulase was produced by endophytic fungus isolated from seagrass. Substrate used for incubating the fungus was the waste of agar industry. The objectives of this study were to optimize cellulase production and to characterize the highest activity of fungal crude cellulase. In our previous study, the EN isolate (isolated from <span style="text-decoration: underline;">Enhalus</span> <span style="text-decoration: underline;">sp</span>.) showed the highest cellulolytic index. Therefore, in this research we focused on cellulase activity of the isolate. Cellulase activity was determined based on endoglucanase activity, total cellulase activity, and β-glucosidase activity. The highest activity was then used to determine cellulase activity in enzyme characterization. The fungus was cultured in different concentration of agar extraction algal wastes. The fungal culture was incubated for 3-21 days with 120 rpm orbital shaker. The results showed that endoglucanase activity was 0.019-0.031 U/mL, total cellulase activity was 0.007-0.013 U/mL, and β-glucosidase activity was 0.00012-0.00361 U/mL. The highest endoglucanase and total cellulase activity were obtained from the fungal culture after 9 days incubation, β-glucosidase was obtained from the fungal culture after 15 days incubation with 1.5% of algal waste as substrate. The optimum pH and temperature were determined as 4 and 60 <sup>o</sup>C, maximum reaction rate (V<sub>max</sub>) and Michaelis-Menten constant (K<sub>m</sub>) for endoglucanase activity was determined as 0.044 U/mL and 0.103% respectively.</p> <p>Keywords: algal waste, cellulase, endophytes, enzymes, seagrass</p>

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