Continuous production ofL-malic acid by immobilizedBrevibacterium ammoniagenes cells

1976 ◽  
Vol 3 (3) ◽  
pp. 169-183 ◽  
Author(s):  
Kozo Yamamoto ◽  
Tetsuya Tosa ◽  
Kiyokazu Yamashita ◽  
Ichiro Chibata
Keyword(s):  
Malic Acid ◽  
Continuous Production

Kinetcs and decay of fumarase activity of immobilizedBrevibacterium ammoniagenes cells for continuous production ofL-malic acid

1977 ◽  
Vol 19 (8) ◽  
pp. 1101-1114 ◽  
Author(s):  
Kozo Yamamoto ◽  
Tetsuya Tosa ◽  
Kiyokazu Yamashita ◽  
Ichiro Chibata
Keyword(s):  
Malic Acid ◽  
Continuous Production ◽  
Fumarase Activity

Design of continuous flow immobilized bacterial cells reactor for production of L-malic acid

1990 ◽  
Vol 55 (9) ◽  
pp. 2192-2198
Author(s):  
Marie Fialová ◽  
Jindřich Zahradník ◽  
Hana Kučerová
Keyword(s):  
Reaction Kinetics ◽  
Malic Acid ◽  
Pilot Plant ◽  
Continuous Production ◽  
Scale Up ◽  
Fixed Bed ◽  
Fixed Bed Reactor ◽  
Design Parameters ◽  
Bacterial Cells ◽  
Effective Reaction

An experimental study was carried out, aimed at establishing an engineering data base for the optimum design of a pilot-plant fixed bed reactor for continuous biotransformation of natrium fumarate to L-malic acid catalysed by immobilized bacterial cells. Cells Corynebacterium sp. immobilized in calcium alginate were used for experiments. Effective reaction kinetics including the effect of transport phenomena in the bed was determined and conditions of the safe conversion data scale up were defined. Regarding the requirements of continuous production, long-term stability of the biocatalytic system was tested at operation conditions corresponding to constant degree of substrate conversion at the reactor outlet. Quantitative evidence on changes of the effective reaction kinetics (i.e. on the decrease of the effective rate constant value) over the three-months production period was obtained from this experimental run. Decisive design parameters of a pilot-plant reactor were calculated for estimated production of 20 t of L-malic acid per 90 days period (maximum time of economic biocatalyst utilization).

Continuous production of L-malic acid by immobilized cells

1983 ◽  
Vol 1 (1) ◽  
pp. 9-11 ◽  
Author(s):  
Ichiro Chibata ◽  
Tetsuya Tosa ◽  
Isao Takata
Keyword(s):  
Malic Acid ◽  
Immobilized Cells ◽  
Continuous Production

Enrichment of common carp (Cyprinus carpio) diet with Malic acid: Effects on skin mucosal immunity, antioxidant defecne and growth performance

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Roghieh Safari ◽  
Seyed Hossein Hoseinifar ◽  
Maryam Dadar ◽  
Hien Van Doan
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Malic Acid ◽  
Control Group ◽  
Skin Mucus ◽  
Antioxidant Genes ◽  
Treatment Comparison ◽  
Genes Expression ◽  
Significant Difference ◽  
And Control

AbstractThe present study investigated possible effects of dietary malic acid on the expression of immunity, antioxidant and growth related genes expression as well as skin mucus immune parameters in common carp. Common carp (Cyprinus carpio) fingerlings were fed diets supplemented with different levels (0 [control], 0.5%, 1%, 2%) of malic acid (MA) for 60 days. The results revealed highest expression levels of immune-related genes (tnf-alpha, il1b, il8 and lyz) in skin of common carp fed 2% MA (P < 0.05). Regarding 1% MA treatment comparison with control group, significant difference was noticed just in case of lyz (P < 0.05). Evaluation of growth related genes expression revealed no significant difference between treatments (P > 0.05). The study of antioxidant related genes (gsta and gpx) in common carp skin fed with MA, showed significant difference between treated groups and control (P < 0.05). Carps fed with 2% MA had highest alkaline phosphatase activity in skin mucus compared other treated groups and control (P < 0.05). There were no significant difference between 0.5% and 1% and control (P > 0.05). The study of total protein and total immunoglobulin (Ig) in common carp skin musus revealed no alteration following MA treatment (P > 0.05). The present data demonstrated that feeding with MA altered immune and antioxidant genes expression in skin mucus of common carp.

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