Microspore development inBrassica napus and the effect of high temperature on division in vivo and in vitro

PROTOPLASMA ◽  
1993 ◽  
Vol 172 (2-4) ◽  
pp. 154-165 ◽  
Author(s):  
Cheryl A. Telmer ◽  
W. Newcomb ◽  
Daina H. Simmonds
Keyword(s):  
High Temperature ◽  
Microspore Development

Study on Assessment of High Temperature and Humidity in Working Environment on Human Health

2012 ◽  
Vol 610-613 ◽  
pp. 739-742 ◽  
Author(s):  
Yu Ping Sun ◽  
Neng Zhu
Keyword(s):  
High Temperature ◽  
Energy Metabolism ◽  
Human Health ◽  
Physiological Responses ◽  
Working Environment ◽  
Heat Diffusion ◽  
Metabolic Heat ◽  
Temperature And Humidity

In this study, a series of references on human physiology and psychology response to hot and humid environments were analyzed. On basis of the thermo metabolism system, energy metabolism system, cardiovascular system and respiratory system, impact mechanism of high temperature and humidity on human health was presented. The results indicate that the high temperature and humidity in working environment have significant impact on human health. The high temperature and humidity cause the reducing of temperature difference in vitro and in vivo, the difficult of metabolic heat diffusion, the significant increasing of energy metabolism and oxygen consumption, heart failure, hypoxia and other physiological responses.

scholarly journals Whole Ovine Ovaries as a Model for Human: Perfusion with CryoprotectantsIn VivoandIn Vitro

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Vladimir Isachenko ◽  
Gohar Rahimi ◽  
Maria Dattena ◽  
Peter Mallmann ◽  
Saltanat Baikoshkarova ◽  
...  
Keyword(s):  
High Temperature ◽  
Calf Serum ◽  
Surgical Removal ◽  
Vascular Pedicle ◽  
Perfusion Medium ◽  
Bovine Calf Serum ◽  
Freezing Medium ◽  
Human Ovaries

These experiments were performed to test the perfusion of ovine as a model for human ovaries by cryoprotectantsin vivoat high temperature when the permeability of capillaries is high and when blood is insensibly replaced by the solution of cryoprotectants. By our hypothetical supposition, ovaries could be saturated by cryoprotectants before their surgical removal. The objective was to examine the effectiveness of perfusion of ovine ovaries with vascular pediclein vivoandin vitro.Arteria ovaricawas cannuled and ovaries were perfused by Leibovitz L-15 medium + 100 IU/mL heparin + 5% bovine calf serum + 6% dimethyl sulfoxide + 6% ethylene glycol + 0.15 M sucrose + Indian inkin vivoandin vitro. In the first and second cycle of experiments, ovaries (n=13andn=23) were perfusedin vivoandin vitro, respectively, during 60 min with the rate of perfusion 50 mL/h (0.8 mL/min). It was established within vivoperfusion that only about 10% of ovarian tissues were perfused due to an appearance of multiple anastomoses when the perfusion medium goes fromarteria ovaricatoarteria uterinawithout inflow into the ovaries. It was concluded thatin vitroperfusion of ovine intact ovaries with vascular pedicle by freezing medium is more effective than this manipulation performedin vivo.

scholarly journals Laboratory characteristics of an attenuated influenza type A (H3N2) virus (‘Alice’ strain)

1976 ◽  
Vol 77 (2) ◽  
pp. 181-188 ◽  
Author(s):  
Michèle Lobmann ◽  
Andrée Delem ◽  
J. Peetermans ◽  
C. Huygelen
Keyword(s):  
Influenza Virus ◽  
High Temperature ◽  
Resistant Strain ◽  
H3n2 Virus ◽  
Virus Recombinant ◽  
Low Sensitivity ◽  
Live Attenuated Influenza Virus ◽  
Selection Of

SummaryThe Alice strain of live attenuated influenza virus was obtained by selection of a γ inhibitor-resistant strain from a virus recombinant between A/PR/8/34 (H0N1) and A/England/42/72 (H3N2). Its behaviour in vitro and in vivo was studied. Three marker systems were investigated: resistance to serum inhibitors, growth capacity at high temperature and low sensitivity to amantadine hydrochloride. In ferrets the strain was found to be attenuated and immunogenic. Passages in man, animals and eggs have not affected its resistance to γ inhibitors.

Quantification of the In Vitro and In Vivo Metabolic Fates of 2-, 3- and 4-Bromobenzoic Acids Using High Temperature LC Coupled to ICP-MS and Linear Ion Trap MS

2008 ◽  
Vol 67 (9-10) ◽  
pp. 673-678 ◽  
Author(s):  
Christopher J. Smith ◽  
Stefanie Shillingford ◽  
Anthony M. Edge ◽  
Christopher Bailey ◽  
Ian D. Wilson
Keyword(s):  
High Temperature ◽  
Ion Trap ◽  
Linear Ion Trap ◽  
Icp Ms

scholarly journals 399 The Mortality of Artificially Infested Third Instar Larvas of Anastrepaha ludens and A. obliqua in Mango Fruit with Insecticidal Controlled Atmospheres at High Temperature

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 512E-512
Author(s):  
Elhadi M. Yahia ◽  
Dora Ortega ◽  
Pamela Moreno ◽  
Alejandro Martinez
Keyword(s):  
High Temperature ◽  
Statistical Analysis ◽  
High Temperatures ◽  
Mango Fruit ◽  
Controlled Atmospheres

Previous work in our laboratory and also reported in this meeting has indicated that insecticidal controlled atmospheres at high temperatures (0.5% O2 + 50% CO2 at 44-55°C and 50% RH) are very effective in causing in vitro mortality of eggs and third instar larvas of Anastrepaha ludens and A. obliqua. This work is a follow up that evaluated the effect of such atmospheres on the in vivo mortality of third instar larvas artificially infested in mango. Atmospheres evaluated included 0% O2+ 50% CO2 at 35, 37, 39, 40, 42, 43, 44, 45, 46, 47, 48, and 49°C for 160 min. Treatments at 35-40°C caused 100% mortality of larvas of A. obliqua, but not of A. ludens. Temperatures of 42 to 49°C caused 100% mortality of larvas of both species. Statistical analysis to calculate the probit 9 will be discussed.

scholarly journals THE RESISTANCE TO A SPECIFIC HEMOLYSIN OF HUMAN ERYTHROCYTES IN HEALTH AND DISEASE

1909 ◽  
Vol 11 (6) ◽  
pp. 763-785 ◽  
Author(s):  
Peyton Rous
Keyword(s):  
High Temperature ◽  
Human Erythrocytes ◽  
The Body ◽  
Biological Reaction ◽  
Health And Disease

1. Phagocytosis of pneumococci in vitro runs parallel with phagocytosis in vivo. 2. Virulence depends not only on resistance to phagocytosis, but also on the ability to grow in the body of the animal. 3. The biological reaction of the pigeon to pneumococcus infection does not differ from that of the mouse. 4. The "immunity" of the pigeon to pneumococcus infection is due to its normal high temperature.

In vitro and in vivo Performance and Lifetime of Perfluorinated Ionomer-Coated Glucose Sensors after High-Temperature Curing

1994 ◽  
Vol 66 (22) ◽  
pp. 3882-3888 ◽  
Author(s):  
Francis. Moussy ◽  
Stephen. Jakeway ◽  
D. Jed. Harrison ◽  
Ray V. Rajotte
Keyword(s):  
High Temperature ◽  
Glucose Sensors

scholarly journals EXPERIMENTAL STUDIES ON PNEUMOCOCCUS INFECTIONS

1909 ◽  
Vol 11 (5) ◽  
pp. 743-761 ◽  
Author(s):  
S. Strouse
Keyword(s):  
High Temperature ◽  
Experimental Studies ◽  
The Body ◽  
Biological Reaction

1. Phagocytosis of pneumococci in vitro runs parallel with phagocytosis in vivo. 2. Virulence depends not only on resistance to phagocytosis, but also on the ability to grow in the body of the animal. 3. The biological reaction of the pigeon to pneumococcus infection does not differ from that of the mouse. 4. The "immunity" of the pigeon to pneumococcus infection is due to its normal high temperature.

Effects of high temperature on grain growth and on the metabolites and enzymes in the starch-synthesis pathway in the grains of two wheat cultivars differing in their responses to temperature

2003 ◽  
Vol 30 (3) ◽  
pp. 291 ◽  
Author(s):  
Morteza Zahedi ◽  
Rajinder Sharma ◽  
Colin F. Jenner
Keyword(s):  
High Temperature ◽  
Starch Synthesis ◽  
Grain Filling ◽  
Triticum Aestivum L ◽  
Soluble Starch ◽  
Starch Synthase ◽  
Effect Of Temperature ◽  
The Difference

The effects of a sustained period of moderately high temperature were evaluated on the availability of substrate and the activity of starch synthase (ADP-glucose: 1,4-α-D-glucan 4-α-D-glucosyltransferase, EC 2.4.1.21) in the developing grains of two wheat Triticum aestivum L. cultivars differing in their tolerance to high temperature. Final grain weight was reduced by 33% in the least sensitive (cv. Kavko) and by 40% in the most sensitive (cv. Lyallpur) cultivar as post-anthesis temperature was raised from 20/15°C (day/night) to 30/25°C. The difference in the response of the two cultivars was mainly due to changes in the rate of grain filling at high temperature. The response of the rate of grain filling at high temperature, and the differential effects on the two cultivars, did not seem to be explained by an effect of temperature on the supply of assimilate (sucrose) or on the availability of the substrate for starch synthesis (ADP-glucose) in the grains. In vitro, but not in vivo, the differential responses of the efficiency (Vmax/Km) of soluble starch synthase in the two cultivars to an increase in temperature were associated with differences in the temperature sensitivity of grain filling. In vivo, the most remarkable difference between the two varieties was in the absolute values of the efficiency of soluble starch synthase, with the most tolerant cultivar having the highest efficiency.

scholarly journals A cold shock protein promotes high-temperature microbial growth through binding to diverse RNA species

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Zikang Zhou ◽  
Hongzhi Tang ◽  
Weiwei Wang ◽  
Lige Zhang ◽  
Fei Su ◽  
...  
Keyword(s):  
High Temperature ◽  
Cold Shock ◽  
Rna Binding ◽  
Cold Shock Protein ◽  
Mrna Accumulation ◽  
Temperature Resistance ◽  
High Temperature Resistance ◽  
Biomass Increase

AbstractEndowing mesophilic microorganisms with high-temperature resistance is highly desirable for industrial microbial fermentation. Here, we report a cold-shock protein (CspL) that is an RNA chaperone protein from a lactate producing thermophile strain (Bacillus coagulans 2–6), which is able to recombinantly confer strong high-temperature resistance to other microorganisms. Transgenic cspL expression massively enhanced high-temperature growth of Escherichia coli (a 2.4-fold biomass increase at 45 °C) and eukaryote Saccharomyces cerevisiae (a 2.6-fold biomass increase at 36 °C). Importantly, we also found that CspL promotes growth rates at normal temperatures. Mechanistically, bio-layer interferometry characterized CspL’s nucleotide-binding functions in vitro, while in vivo we used RNA-Seq and RIP-Seq to reveal CspL’s global effects on mRNA accumulation and CspL’s direct RNA binding targets, respectively. Thus, beyond establishing how a cold-shock protein chaperone provides high-temperature resistance, our study introduces a strategy that may facilitate industrial thermal fermentation.

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