In vitro crystallisation systems for the study of urinary stone formation

1997 ◽  
Vol 15 (4) ◽  
pp. 244-251 ◽  
Author(s):  
W. Achilles
Keyword(s):  
Stone Formation ◽  
Urinary Stone

scholarly journals Effect of Curcumin AgainstProteus mirabilisDuring Crystallization of Struvite from Artificial Urine

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Jolanta Prywer ◽  
Agnieszka Torzewska
Keyword(s):  
Urinary Tract Infection ◽  
Proteus Mirabilis ◽  
Crystallization Process ◽  
Crystal Morphology ◽  
Stone Formation ◽  
Urinary Stone ◽  
Urinary Stones ◽  
Artificial Urine ◽  
Struvite Crystallization

We investigated the activity of curcumin againstProteus mirabilisand the struvite crystallization in relation to urinary stones formation. In order to evaluate an activity of curcumin we performed anin vitroexperiment of struvite growth from artificial urine. The crystallization process was induced byProteus mirabilisto mimic the real urinary tract infection, which usually leads to urinary stone formation. The results demonstrate that curcumin exhibits the effect againstProteus mirabilisinhibiting the activity of urease—an enzyme produced by these microorganisms. Addition of curcumin increases the induction time and decreases the efficiency of growth of struvite compared with the absence of curcumin. Interestingly, the addition of curcumin does not affect the crystal morphology and habit. In conclusion, curcumin has demonstrated its significant potential to be further investigated for its use in the case of struvite crystallization induced for the growth byProteus mirabilisin relation to urinary stone formation.

scholarly journals IN VITRO ANTIUROLITHIATIC ACTIVITY OF MACERATED AQUEOUS EXTRACT OF TERMINALIA BELERICA BY USING TURBIDITY METHOD

2021 ◽  
pp. 87-89
Author(s):  
ANU V. ◽  
AKHILA S. ◽  
SNEHA ANTONY
Keyword(s):  
Aqueous Extract ◽  
Stone Formation ◽  
Inhibitory Effect ◽  
Urinary Stone ◽  
Positive Control ◽  
Standard Drug ◽  
Terminalia Belerica ◽  
Turbidity Method ◽  
Medicinal Drugs

Objective: To evaluate the anti-urolithiatic activity of macerated aqueous extract of Terminalia belerica by using turbidity method Method: The present study was used to study the inhibitory effect of the Terminalia belerica on urinary stone formation. The aim of study was to examine the In vitro antiurolithiatic activity of macerated aqueous extract of T.belerica was to estimate inhibitory activity of aqueous extract on the formation of urinary stone. Cystone was used as a positive control. Anti urolithiatic study was performed by turbidity method. Result: The percentage inhibition shown by aqueous extract at 20μg/ml was 60% and with almost constant inhibition at 100μg/ml and 200μg/ml ranging between 72% and 80%. The percentage inhibition showed by aqueous extract of Terminalia belerica has significant compared to standard drug. Conclusion: In future this drug can be performed in vitro and clinical study beneficial for people with avoiding adverse effect of modern medicinal drugs

scholarly journals Correlation between Ion Composition of Oligomineral Water and Calcium Oxalate Crystal Formation

Crystals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1507
Author(s):  
Manuela Rossi ◽  
Biagio Barone ◽  
Dante Di Domenico ◽  
Rodolfo Esposito ◽  
Antonio Fabozzi ◽  
...  
Keyword(s):  
Calcium Oxalate ◽  
Aqueous Medium ◽  
Stone Formation ◽  
Ionic Species ◽  
Urinary Stone ◽  
Crystal Formation ◽  
Ion Composition ◽  
Calcium Oxalate Crystal ◽  
Ion Content

The ion content of drinking water might be associated with urinary stone formation, representing a keystone of conservative nephrolithiasis management. However, the effects of specific ions on calcium oxalate crystal formation and their mechanism of action are still highly controversial. We report an investigation of the effects of oligomineral waters with similar total salt amount but different ion composition on calcium oxalate (CaOx) precipitation in vitro, combining gravimetric and microscopic assays. The results suggest that the “collective” physicochemical properties of the aqueous medium, deriving from the ion combination rather than from a single ionic species, are of importance. Particularly, the ability of ions to strengthen/weaken the aqueous medium structure determines an increase/decrease in the interfacial energy, modulating the formation and growth of CaOx crystals.

scholarly journals Proteus mirabilis Urease: Unsuspected Non-Enzymatic Properties Relevant to Pathogenicity

2021 ◽  
Vol 22 (13) ◽  
pp. 7205
Author(s):  
Matheus V. C. Grahl ◽  
Augusto F. Uberti ◽  
Valquiria Broll ◽  
Paula Bacaicoa-Caruso ◽  
Evelin F. Meirelles ◽  
...  
Keyword(s):  
Proteus Mirabilis ◽  
Stone Formation ◽  
Cell Types ◽  
Bioinformatic Analysis ◽  
Urinary Stone ◽  
Enzymatic Properties ◽  
Hek293 Cells ◽  
Urinary Stones ◽  
Isolated Cells ◽  
Tnf Α

Infection by Proteus mirabilis causes urinary stones and catheter incrustation due to ammonia formed by urease (PMU), one of its virulence factors. Non-enzymatic properties, such as pro-inflammatory and neurotoxic activities, were previously reported for distinct ureases, including that of the gastric pathogen Helicobacter pylori. Here, PMU was assayed on isolated cells to evaluate its non-enzymatic properties. Purified PMU (nanomolar range) was tested in human (platelets, HEK293 and SH-SY5Y) cells, and in murine microglia (BV-2). PMU promoted platelet aggregation. It did not affect cellular viability and no ammonia was detected in the cultures’ supernatants. PMU-treated HEK293 cells acquired a pro-inflammatory phenotype, producing reactive oxygen species (ROS) and cytokines IL-1β and TNF-α. SH-SY5Y cells stimulated with PMU showed high levels of intracellular Ca2+ and ROS production, but unlike BV-2 cells, SH-SY5Y did not synthesize TNF-α and IL-1β. Texas Red-labeled PMU was found in the cytoplasm and in the nucleus of all cell types. Bioinformatic analysis revealed two bipartite nuclear localization sequences in PMU. We have shown that PMU, besides urinary stone formation, can potentially contribute in other ways to pathogenesis. Our data suggest that PMU triggers pro-inflammatory effects and may affect cells beyond the renal system, indicating a possible role in extra-urinary diseases.

scholarly journals Nutrition and Kidney Stone Disease

Nutrients ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1917
Author(s):  
Roswitha Siener
Keyword(s):  
Kidney Stone ◽  
Fluid Intake ◽  
Stone Formation ◽  
Urinary Stone ◽  
Stone Disease ◽  
Urinary Stones ◽  
Dietary Therapy ◽  
Effective Prevention ◽  
Kidney Stone Disease ◽  
Nutrition And Diet

The prevalence of kidney stone disease is increasing worldwide. The recurrence rate of urinary stones is estimated to be up to 50%. Nephrolithiasis is associated with increased risk of chronic and end stage kidney disease. Diet composition is considered to play a crucial role in urinary stone formation. There is strong evidence that an inadequate fluid intake is the major dietary risk factor for urolithiasis. While the benefit of high fluid intake has been confirmed, the effect of different beverages, such as tap water, mineral water, fruit juices, soft drinks, tea and coffee, are debated. Other nutritional factors, including dietary protein, carbohydrates, oxalate, calcium and sodium chloride can also modulate the urinary risk profile and contribute to the risk of kidney stone formation. The assessment of nutritional risk factors is an essential component in the specific dietary therapy of kidney stone patients. An appropriate dietary intervention can contribute to the effective prevention of recurrent stones and reduce the burden of invasive surgical procedures for the treatment of urinary stone disease. This narrative review has intended to provide a comprehensive and updated overview on the role of nutrition and diet in kidney stone disease.

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