Antigenic variation of avian infectious bronchitis virus during replication in BHK-21 cells

1982 ◽  
Vol 73 (1) ◽  
pp. 75-78 ◽  
Author(s):  
K. Otsuki ◽  
Y. Tagawa ◽  
M. Tsubokura
Keyword(s):  
Infectious Bronchitis Virus ◽  
Antigenic Variation ◽  
Avian Infectious Bronchitis Virus ◽  
Infectious Bronchitis

scholarly journals A Novel Immunochromatographic Strip for Antigen Detection of Avian Infectious Bronchitis Virus

2019 ◽  
Vol 20 (9) ◽  
pp. 2216 ◽  
Author(s):  
I-Li Liu ◽  
Yi-Chun Lin ◽  
Yong-Chong Lin ◽  
Cai-Zhen Jian ◽  
Ivan-Chen Cheng ◽  
...  
Keyword(s):  
Infectious Bronchitis Virus ◽  
Antigenic Variation ◽  
Viral Evolution ◽  
Rapid Test ◽  
High Specificity ◽  
Antigen Detection ◽  
Economic Losses ◽  
Avian Infectious Bronchitis Virus ◽  
Immunochromatographic Strip ◽  
Infectious Bronchitis

Avian infectious bronchitis virus (IBV) causes considerable economic losses in the poultry industry worldwide, including Taiwan. IBV is among the most important pathogens in chickens, and it spreads rapidly among flocks. In addition to dozens of known serotypes, new viral variants have emerged due to the viral evolution and antigenic variation in IBVs. Therefore, the development of a sensitive, specific, and easily performed assay is crucial for the rapid detection and surveillance of IBV infections. A rapid and simple immunochromatographic strip (ICS) was developed in this study by employing monoclonal antibodies against spike and nucleocapsid proteins of IBV as the tracer and the capture antibody. The ICS showed high specificity in detecting IBV antigens, including several IBV genotypes and novel variants, as opposed to three other common avian respiratory viruses. The detection limit of the strip reached 104.4 50% embryo-infective dose. Moreover, in the experimental chicken model, the strip test demonstrated consistency in detecting IBV with RT-PCR gene detection. Taken together, this antigen detection strip has the potential to serve as an on-farm rapid test for IBV; therefore, it may facilitate surveillance and control of the disease.

Comparative Evaluation of In Vitro and In Vivo Assays for the Detection of Avian Infectious Bronchitis Virus as a Contaminant of Live Poultry Vaccines

1998 ◽  
Vol 26 (5) ◽  
pp. 629-634
Author(s):  
Emiliana Falcone ◽  
Edoardo Vignolo ◽  
Livia Di Trani ◽  
Simona Puzelli ◽  
Maria Tollis
Keyword(s):  
Infectious Bronchitis Virus ◽  
Serological Response ◽  
Avian Infectious Bronchitis Virus ◽  
Animal Testing ◽  
Rt Pcr ◽  
Pcr Assay ◽  
In Vivo Test ◽  
Infectious Bronchitis

A reverse transcriptase polymerase chain reaction (RT-PCR) assay specific for identifying avian infectious bronchitis virus (IBV) in poultry vaccines, and the serological response to IBV induced by the inoculation of chicks with a Newcastle disease vaccine spiked with the Massachusetts strain of IBV, were compared for their ability to detect IBV as a contaminant of avian vaccines. The sensitivity of the IBV-RT-PCR assay provided results which were at least equivalent to the biological effect produced by the inoculation of chicks, allowing this assay to be considered a valid alternative to animal testing in the quality control of avian immunologicals. This procedure can easily be adapted to detect a number of contaminants for which the in vivo test still represents the only available method of detection.

Avian infectious bronchitis virus: isolation of an apparently new variant in Italy

2000 ◽  
Vol 146 (7) ◽  
pp. 191-193 ◽  
Author(s):  
A. Zanella ◽  
R. Coaro ◽  
R. Marchi ◽  
G. Fabris ◽  
A. Lavazza
Keyword(s):  
Infectious Bronchitis Virus ◽  
Virus Isolation ◽  
Avian Infectious Bronchitis Virus ◽  
Infectious Bronchitis ◽  
New Variant

scholarly journals Research Note: Antibodies to Avian Infectious Bronchitis Virus in Pakistani Chickens

1987 ◽  
Vol 66 (4) ◽  
pp. 765-767 ◽  
Author(s):  
MOHAMMAD A. MUNEER ◽  
JOHN A. NEWMAN ◽  
SAGAR M. GOYAL ◽  
M. AJMAL
Keyword(s):  
Infectious Bronchitis Virus ◽  
Research Note ◽  
Avian Infectious Bronchitis Virus ◽  
Infectious Bronchitis
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