Antigenic relationships between rotaviruses from different species as studied by neutralization and immunofluorescence

1982 ◽  
Vol 73 (1) ◽  
pp. 45-50 ◽  
Author(s):  
K. Sato ◽  
Y. Inaba ◽  
Y. Miura ◽  
S. Tokuhisa ◽  
M. Matumoto
Keyword(s):  
Antigenic Relationships

Host range, RFLP, and antigenic relationships betweenRhizobium fredii strains andRhizobium sp. NGR234

1994 ◽  
Vol 161 (1) ◽  
pp. 21-29 ◽  
Author(s):  
Hari B. Krishnan ◽  
Steven G. Pueppke
Keyword(s):  
Host Range ◽  
Antigenic Relationships

scholarly journals Antigenic diversity and seroprevalences of Torque teno viruses in children and adults by ORF2-based immunoassays

2013 ◽  
Vol 94 (2) ◽  
pp. 409-417 ◽  
Author(s):  
Tingting Chen ◽  
Elina Väisänen ◽  
Petri S. Mattila ◽  
Klaus Hedman ◽  
Maria Söderlund-Venermo
Keyword(s):  
Serum Samples ◽  
Phylogenetic Groups ◽  
Single Strain ◽  
New Approach ◽  
Antiviral Antibody ◽  
Specific Igg ◽  
Species Specific ◽  
Multiple Strains ◽  
Antigenic Relationships

Torque teno viruses (TTVs) circulate widely among humans, causing persistent viraemia in healthy individuals. Numerous TTV isolates with high genetic variability have been identified and segregated into 29 species of five major phylogenetic groups. To date, the diversity of TTV sequences, challenges in protein expression and the subsequent lack of serological assays have hampered TTV seroprevalence studies. Moreover, the antigenic relationships of different TTVs and their specific seroprevalences in humans remain unknown. For five TTV strains – belonging to different species of four genogroups – we developed, using recombinant glutathione S-transferase (GST)-fused TTV ORF2 proteins, glutathione–GST capture enzyme immunoassays (EIAs) detecting antibodies towards conformational epitopes. We then analysed serum samples from 178 healthy adults and 108 children; IgG reactivities were observed either towards a single strain or towards multiple strains, which pointed to antigenic distinction of TTV species. The overall seroprevalence for the five TTVs peaked at 43 % (18 of 42) in children 2–4 years of age, subsequently declined, and again reached 42 % (74 of 178) among adults. TTV6 species-specific IgG predominated in children, whereas that for TTV13 predominated in adults. During a 3 year follow-up of the same children, both species-specific seroconversions and seroreversions occurred. This is the first EIA-based study of different TTVs, providing a new approach for seroepidemiology and diagnosis of TTV infections. Our data suggest that different TTVs in humans may differ in antiviral antibody profiles, infection patterns and epidemiology.

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