Generalized Legendre's associated functions (Integral theorems, recurrence formulas)

1959 ◽  
Vol 63 (1) ◽  
pp. 24-31 ◽  
Author(s):  
L. Kuipers
Keyword(s):  
Recurrence Formulas ◽  
Associated Functions

Importance of Zebrafish as an efficient research model for the screening of novel therapeutics in neurological disorders

2020 ◽  
Vol 19 ◽  
Author(s):  
Diksha Saluja ◽  
Rishabh Jhanji ◽  
Swati Kaushal ◽  
Bharti Verma ◽  
Neelam Sharma ◽  
...  
Keyword(s):  
Animal Model ◽  
Neurological Disorders ◽  
Tropical Fish ◽  
Mammalian Brain ◽  
Behavioral Repertoire ◽  
Experimental Procedure ◽  
Novel Drugs ◽  
Associated Functions ◽  
Pharmacology And Toxicology ◽  
Rats And Mice

Abstract:: In the previous years of research, the use of animal model becomes very common for the screening of novel drugs. Animal model represents the complex problems of humans into simplest forms which can be extended further to include the experimental procedure. The most successful models in neuroscience, rats and mice, undoubtedly considered as one of the best models to understand the psychology of mammalian brain and its associated functions involved in various behavioral repertoire. Moreover, recently researchers in behavioral neuroscience are focusing more on the use of aquatic animals especially fish as model species due to their simplicity, and cost effectiveness. Zebrafish (Danio rerio) is a tropical fish from minnow family a genetic structure surprisingly 84 % similar to humans. It is gaining popularity as a model to study the mechanism in behavioral neuropharmacology. Moreover, Zebrafish is having numerous advantages over other rodent models like ease in maintenance due to their small size; breeding power is more, transparency of embryos, overall reduced cost of experimentation and many more. Nowadays, it is considered as an ideal model to study the neurobehavioral aspects with relevance to humans. It is also used in varieties of scientific studies like genetics, neuroscience, pharmacology, and toxicology. In this manuscript, we have described the feasibility and importance of Zebrafish as a model for the screening of novel drugs for different neurological disorders.

scholarly journals Burkholderia cenocepacia transcriptome during the early contacts with giant plasma membrane vesicles derived from live bronchial epithelial cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Andreia I. Pimenta ◽  
Nuno Bernardes ◽  
Marta M. Alves ◽  
Dalila Mil-Homens ◽  
Arsenio M. Fialho
Keyword(s):  
Membrane Vesicles ◽  
Bronchial Epithelial Cell ◽  
Host Cells ◽  
Burkholderia Cenocepacia ◽  
Transport Systems ◽  
Epithelial Cell Line ◽  
Plasma Membrane Vesicles ◽  
Bronchial Epithelial ◽  
Cellular Processes ◽  
Associated Functions

AbstractBurkholderia cenocepacia is known for its capacity of adherence and interaction with the host, causing severe opportunistic lung infections in cystic fibrosis patients. In this work we produced Giant Plasma Membrane Vesicles (GPMVs) from a bronchial epithelial cell line and validated their use as a cell-like alternative to investigate the steps involved in the adhesion process of B. cenocepacia. RNA-sequencing was performed and the analysis of the B. cenocepacia K56-2 transcriptome after the first contacts with the surface of host cells allowed the recognition of genes implicated in bacterial adaptation and virulence-associated functions. The sensing of host membranes led to a transcriptional shift that caused a cascade of metabolic and physiological adaptations to the host specific environment. Many of the differentially expressed genes encode proteins related with central metabolic pathways, transport systems, cellular processes, and virulence traits. The understanding of the changes in gene expression that occur in the early steps of infection can uncover new proteins implicated in B. cenocepacia-host cell adhesion, against which new blocking agents could be designed to control the progression of the infectious process.

scholarly journals SIMULTANEOUS DEFINITION OF KEY CHARACTERISTICS IN ORDER TO FACILITATE ROBUST DESIGN IN EARLY PRODUCT DEVELOPMENT STAGES

2021 ◽  
Vol 1 ◽  
pp. 2691-2700
Author(s):  
Stefan Goetz ◽  
Dennis Horber ◽  
Benjamin Schleich ◽  
Sandro Wartzack
Keyword(s):  
Product Development ◽  
Robust Design ◽  
Language Processing ◽  
Design Stage ◽  
Concept Design ◽  
Clear Definition ◽  
Implicit Information ◽  
Key Characteristics ◽  
Associated Functions ◽  
Definition Of

AbstractThe success of complex product development projects strongly depends on the clear definition of target factors that allow a reliable statement about the fulfilment of the product requirements. In the context of tolerancing and robust design, Key Characteristics (KCs) have been established for this purpose and form the basis for all downstream activities. In order to integrate the activities related to the KC definition into product development as early as possible, the often vaguely formulated requirements must be translated into quantifiable KCs. However, this is primarily a manual process, so the results strongly depend on the experience of the design engineer.In order to overcome this problem, a novel computer-aided approach is presented, which automatically derives associated functions and KCs already during the definition of product requirements. The approach uses natural language processing and formalized design knowledge to extract and provide implicit information from the requirements. This leads to a clear definition of the requirements and KCs and thus creates a founded basis for robustness evaluation at the beginning of the concept design stage. The approach is exemplarily applied to a window lifter.

scholarly journals Exploring New Scaffolds for the Dual Inhibition of HIV-1 RT Polymerase and Ribonuclease Associated Functions

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3821
Author(s):  
Rita Meleddu ◽  
Angela Corona ◽  
Simona Distinto ◽  
Filippo Cottiglia ◽  
Serenella Deplano ◽  
...  
Keyword(s):  
Single Agent ◽  
Hiv Reverse Transcriptase ◽  
Substitution Pattern ◽  
Associated Functions ◽  
Dual Inhibition ◽  
Hiv Rt ◽  
Therapeutic Protocols ◽  
Anti Hiv ◽  
Hiv 1 ◽  
Selection Of

Current therapeutic protocols for the treatment of HIV infection consist of the combination of diverse anti-retroviral drugs in order to reduce the selection of resistant mutants and to allow for the use of lower doses of each single agent to reduce toxicity. However, avoiding drugs interactions and patient compliance are issues not fully accomplished so far. Pursuing on our investigation on potential anti HIV multi-target agents we have designed and synthesized a small library of biphenylhydrazo 4-arylthiazoles derivatives and evaluated to investigate the ability of the new derivatives to simultaneously inhibit both associated functions of HIV reverse transcriptase. All compounds were active towards the two functions, although at different concentrations. The substitution pattern on the biphenyl moiety appears relevant to determine the activity. In particular, compound 2-{3-[(2-{4-[4-(hydroxynitroso)phenyl]-1,3-thiazol-2-yl} hydrazin-1-ylidene) methyl]-4-methoxyphenyl} benzamide bromide (EMAC2063) was the most potent towards RNaseH (IC50 = 4.5 mM)- and RDDP (IC50 = 8.0 mM) HIV RT-associated functions.

scholarly journals Targeting DNA Methylation Depletes Uterine Leiomyoma Stem Cell–enriched Population by Stimulating Their Differentiation

Endocrinology ◽  
2020 ◽  
Vol 161 (10) ◽  
Author(s):  
Shimeng Liu ◽  
Ping Yin ◽  
Jingting Xu ◽  
Ariel J Dotts ◽  
Stacy A Kujawa ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Stem Cell ◽  
Cell Population ◽  
Uterine Leiomyoma ◽  
Stem Cell Population ◽  
Epigenetic Landscape ◽  
Stem Cell Regulation ◽  
Integrative Analyses ◽  
Associated Functions ◽  
Intermediate Cells

Abstract Uterine leiomyoma (LM) is the most common tumor in women and can cause severe morbidity. Leiomyoma growth requires the maintenance and proliferation of a stem cell population. Dysregulated deoxyribonucleic acid (DNA) methylation has been reported in LM, but its role in LM stem cell regulation remains unclear. Here, we fluorescence-activated cell sorting (FACS)-sorted cells from human LM tissues into 3 populations: LM stem cell–like cells (LSC, 5%), LM intermediate cells (LIC, 7%), and differentiated LM cells (LDC, 88%), and we analyzed the transcriptome and epigenetic landscape of LM cells at different differentiation stages. Leiomyoma stem cell–like cells harbored a unique methylome, with 8862 differentially methylated regions compared to LIC and 9444 compared to LDC, most of which were hypermethylated. Consistent with global hypermethylation, transcript levels of TET1 and TET3 methylcytosine dioxygenases were lower in LSC. Integrative analyses revealed an inverse relationship between methylation and gene expression changes during LSC differentiation. In LSC, hypermethylation suppressed the genes important for myometrium- and LM-associated functions, including muscle contraction and hormone action, to maintain stemness. The hypomethylating drug, 5′-Aza, stimulated LSC differentiation, depleting the stem cell population and inhibiting tumor initiation. Our data suggest that DNA methylation maintains the pool of LSC, which is critical for the regeneration of LM tumors.

scholarly journals Chromatin-Associated Proteins Revealed by SILAC-Proteomic Analysis Exhibit a High Likelihood of Requirement for Growth Fitness under DNA Damage Stress

2012 ◽  
Vol 2012 ◽  
pp. 1-12
Author(s):  
Han Wang ◽  
Pornpimol Tipthara ◽  
Lei Zhu ◽  
Suk Yean Poon ◽  
Kai Tang ◽  
...  
Keyword(s):  
Dna Damage ◽  
Proteomic Analysis ◽  
Ribosomal Proteins ◽  
Cell Extract ◽  
Label Free ◽  
High Likelihood ◽  
Nonhistone Proteins ◽  
Quantitative Proteomic Analysis ◽  
Proteomic Approach ◽  
Associated Functions

Chromatin-associated nonhistone proteins (CHRAPs) are readily collected from the DNaseI digested crude chromatin preparation. In this study, we show that the absolute abundance-based label-free quantitative proteomic analysis fail to identify potential CHRAPs from the CHRAP-prep. This is because that the most-highly abundant cytoplasmic proteins such as ribosomal proteins are not effectively depleted in the CHRAP-prep. Ribosomal proteins remain the top-ranked abundant proteins in the CHRAP-prep. On the other hand, we show that relative abundance-based SILAC-mediated quantitative proteomic analysis is capable of discovering the potential CHRAPs in the CHRAP-prep when compared to the whole-cell-extract. Ribosomal proteins are depleted from the top SILAC ratio-ranked proteins. In contrast, nucleus-localized proteins or potential CHRAPs are enriched in the top SILAC-ranked proteins. Consistent with this, gene-ontology analysis indicates that CHRAP-associated functions such as transcription, regulation of chromatin structures, and DNA replication and repair are significantly overrepresented in the top SILAC-ranked proteins. Some of the novel CHRAPs are confirmed using the traditional method. Notably, phenotypic assessment reveals that the top SILAC-ranked proteins exhibit the high likelihood of requirement for growth fitness under DNA damage stress. Taken together, our results indicate that the SILAC-mediated proteomic approach is capable of determining CHRAPs without prior knowledge.

Development of a Brassica seed cDNA microarray

Genome ◽  
2008 ◽  
Vol 51 (3) ◽  
pp. 236-242 ◽  
Author(s):  
Daoquan Xiang ◽  
Raju Datla ◽  
Fengling Li ◽  
Adrian Cutler ◽  
Meghna R. Malik ◽  
...  
Keyword(s):  
Expression Patterns ◽  
Cdna Array ◽  
Brassica Species ◽  
Crop Species ◽  
Rna Targets ◽  
Associated Functions ◽  
Leaf Tissues ◽  
Germinating Seeds ◽  
Signal Intensities

Brassica species represent several important crops including canola ( Brassica napus ). Understanding of genetic elements that contribute to seed-associated functions will impact future improvements in the canola crop. Brassica species share a very close taxonomic and molecular relationship with Arabidopsis thaliana. However, there are several subtle but distinct seed-associated agronomic characteristics that differ among the oil seed crop species. To address these, we have generated 67 535 ESTs predominately from Brassica seeds, analyzed these sequences, and identified 10 642 unigenes for the preparation of a targeted seed cDNA array. A set of 10 642 PCR primer pairs was designed and corresponding amplicons were produced for spotting, along with relevant controls. Critical quality control tests produced satisfactory results for use of this microarray in biological experiments. The microarray was also tested with specific RNA targets from embryos, germinating seeds, and leaf tissues. The hybridizations, signal intensities, and overall quality of these slides were consistent and reproducible. Additionally, there are 429 ESTs represented on the array that show no homology with any A. thaliana annotated gene or any gene in the Brassica genome databases or other plant databases; however, all of these probes hybridized to B. napus transcripts, indicating that the array also will be useful in defining expression patterns for genes so far unique to Brassica species.

Analysis for understanding and standardization of additive manufacturing processes

2021 ◽  
Author(s):  
Alexander Matschinski ◽  
Tim Osswald ◽  
Klaus Drechsler
Keyword(s):  
Additive Manufacturing ◽  
High Performance ◽  
Manufacturing Systems ◽  
Polymer Melt ◽  
Thermal Shrinkage ◽  
Target Component ◽  
Associated Functions ◽  
Cooling Mechanism ◽  
Characteristic Values ◽  
As Stress

The market segment of additive manufacturing is showing an annual growth of more than ten percent, with extrusion-based processes being the larger segment of the market. The scope of use is limited to secondary structures. Equipment manufacturers try to guarantee constant material characteristics by closed systems. The characteristic values are up to 50% below the ones from injection molding. The processing of high-performance polymers with reinforcing fibers is an additional challenge. Further development requires an opening of the material and manufacturing systems. The guidelines and standardization for this are still missing. For this reason, a functional analysis (FA) according to TRIZ ("theory of the resolution of invention-related tasks") is performed within this study. This identifies the undesired functions and quantifies their coupling with process components and parameters. In the FA, the manufactured part is the target component in order to address its quality. This way the FA identifies five undesirable functions in the process. These are: deform, cool, weaken, swell and shape. For hightemperature thermoplastics, thermal shrinkage is the primary cause of geometric tolerance. Therefore, the deformation is largely dependent on the cooling mechanism. For a detailed analysis, the polymer melt is further disassembled. The results are six sub-components. The weakening is mainly due to the physical phase of the voids, which exists during the entire processing. The breakdown comprises physical fields such as stress, temperature and flow. These determine the output properties as well as the bonding between the layers. The associated functions are the swelling and shaping. In order to generate broadly applicable standardizations, research questions for further investigation are derived from this study.

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