W-reactivation of phage lambda in X-irradiated mutants ofEscherichia coli K-12

1980 ◽  
Vol 18 (1) ◽  
pp. 27-36 ◽  
Author(s):  
K. D. Martignoni ◽  
I. Haselbacher
Keyword(s):  
Ofescherichia Coli ◽  
Phage Lambda ◽  
K 12

scholarly journals Genetic studies of strain Bs8 ofEscherichia coli

1968 ◽  
Vol 12 (1) ◽  
pp. 55-63 ◽  
Author(s):  
John Donch ◽  
Joseph Greenberg
Keyword(s):  
Radiation Resistance ◽  
Resistant Strain ◽  
Double Mutant ◽  
Ofescherichia Coli ◽  
Genetic Studies ◽  
Radiation Resistant ◽  
K 12

Strain Bs8 is a u.v.-sensitive derivative of strain B. It is unable to reactivate irradiated phage (HCR) and it cannot be induced to form filaments. The HCR properties are attributable to a gene,uvr8, cotransducible withgal+. Whenuvr8was transduced into radiation-resistant strain B/r the resulting phenotype was indistinguishable from Bs8. When transduced into alonK-12 strain the phenotype was more sensitive than Bs8, filament-inducible and mucoid. When PI-Bs8 was used to transduceproC+into aproCK-12 strain, 4% of the transductants werelon, i.e. about as u.v.-sensitive as Ion K-12, filament-inducible and mucoid. Radiation resistance could not be transduced from strain Bs8 withproC+into aproClon K-12 derivative. Nor did Bs8 show any evidence of being exr. Bs8 is a double mutant of strain B, behaving as though it were HCE, in a B/r background.

Effects of different culture media and oxygen upon lipids ofEscherichia coli K-12

Lipids ◽  
1974 ◽  
Vol 9 (5) ◽  
pp. 322-327 ◽  
Author(s):  
William F. Naccarato ◽  
John R. Gilbertson ◽  
Rose A. Gelman
Keyword(s):  
Culture Media ◽  
Ofescherichia Coli ◽  
K 12

Expression, isolation and properties of Fur (ferric uptake regulation) protein ofEscherichia coli K 12

1988 ◽  
Vol 1 (1) ◽  
pp. 62-68 ◽  
Author(s):  
Sechan Wee ◽  
J. B. Neilands ◽  
Michael L. Bittner ◽  
Bruce C. Hemming ◽  
Barry L. Haymore ◽  
...  
Keyword(s):  
Ofescherichia Coli ◽  
K 12

scholarly journals First-Time Isolation and Characterization of a Bacteriophage Encoding the Shiga Toxin 2c Variant, Which Is Globally Spread in Strains of Escherichia coli O157

2004 ◽  
Vol 72 (12) ◽  
pp. 7030-7039 ◽  
Author(s):  
Eckhard Strauch ◽  
Christoph Schaudinn ◽  
Lothar Beutin
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Phage Lambda ◽  
Wild Type ◽  
Chromosomal Locus ◽  
O157 Strain ◽  
Diagnostic Pcr ◽  
E Coli ◽  
Isolation And Characterization ◽  
K 12

ABSTRACT A bacteriophage encoding the Shiga toxin 2c variant (Stx2c) was isolated from the human Escherichia coli O157 strain CB2851 and shown to form lysogens on the E. coli K-12 laboratory strains C600 and MG1655. Production of Stx2c was found in the wild-type E. coli O157 strain and the K-12 lysogens and was inducible by growing bacteria in the presence of ciprofloxacin. Phage 2851 is the first reported viable bacteriophage which carries an stx 2c gene. Electron micrographs of phage 2851 showed particles with elongated hexagonal heads and long flexible tails resembling phage lambda. Sequence analysis of an 8.4-kb region flanking the stx 2c gene and other genetic elements revealed a mosaic gene structure, as found in other Stx phages. Phage 2851 showed lysis of E. coli K-12 strains lysogenic for Stx phages encoding Stx1 (H19), Stx2 (933W), Stx (7888), and Stx1c (6220) but showed superinfection immunity with phage lambda, presumably originating from the similarity of the cI repressor proteins of both phages. Apparently, phage 2851 integrates at a different chromosomal locus than Stx2 phage 933W and Stx1 phage H19 in E. coli, explaining why Stx2c is often found in combination with Stx1 or Stx2 in E. coli O157 strains. Diagnostic PCR was performed to determine gene sequences specific for phage 2851 in wild-type E. coli O157 strains producing Stx2c. The phage 2851 q and o genes were frequently detected in Stx2c-producing E. coli O157 strains, indicating that phages related to 2851 are associated with Stx2c production in strains of E. coli O157 that were isolated in different locations and time periods.

Comparing the predicted and observed properties of proteins encoded in the genome ofEscherichia coli K-12

1997 ◽  
Vol 18 (8) ◽  
pp. 1259-1313 ◽  
Author(s):  
Andrew J. Link ◽  
Keith Robison ◽  
George M. Church
Keyword(s):  
Ofescherichia Coli ◽  
K 12

scholarly journals Activation by Gene Amplification ofpitB, Encoding a Third Phosphate Transporter ofEscherichia coli K-12

2001 ◽  
Vol 183 (15) ◽  
pp. 4659-4663 ◽  
Author(s):  
Sally M. Hoffer ◽  
Paul Schoondermark ◽  
Hendrik W. van Veen ◽  
Jan Tommassen
Keyword(s):  
Escherichia Coli ◽  
Gene Amplification ◽  
Inorganic Phosphate ◽  
Double Mutant ◽  
Phosphate Transporter ◽  
Ofescherichia Coli ◽  
Two Systems ◽  
K 12

ABSTRACT Two systems for the uptake of inorganic phosphate (Pi) in Escherichia coli, PitA and Pst, have been described. A revertant of a pitA pstS double mutant that could grow on Pi was isolated. We demonstrate that the expression of a new Pi transporter, PitB, is activated in this strain by a gene amplification event.

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