Cytoplasmic streaming in internodal cells ofNitella under centrifugal acceleration: a study done with a newly constructed centrifuge microscope

PROTOPLASMA ◽  
1989 ◽  
Vol 152 (2-3) ◽  
pp. 148-155 ◽  
Author(s):  
E. Kamitsubo ◽  
Y. Ohashi ◽  
M. Kikuyama
Keyword(s):  
Cytoplasmic Streaming ◽  
Centrifugal Acceleration ◽  
Centrifuge Microscope

Ultrastructural Investigations of the Contractile Filaments of Amoebae

1974 ◽  
Vol 32 ◽  
pp. 188-189
Author(s):  
P.L. Moore
Keyword(s):  
Cytoplasmic Streaming ◽  
Three Dimensional ◽  
Freeze Fracture ◽  
Amoeboid Movement ◽  
Different Types ◽  
Chemical Conditions ◽  
Complete Interpretation

Previous freeze fracture results on the intact giant, amoeba Chaos carolinensis indicated the presence of a fibrillar arrangement of filaments within the cytoplasm. A complete interpretation of the three dimensional ultrastructure of these structures, and their possible role in amoeboid movement was not possible, since comparable results could not be obtained with conventional fixation of intact amoebae. Progress in interpreting the freeze fracture images of amoebae required a more thorough understanding of the different types of filaments present in amoebae, and of the ways in which they could be organized while remaining functional.The recent development of a calcium sensitive, demembranated, amoeboid model of Chaos carolinensis has made it possible to achieve a better understanding of such functional arrangements of amoeboid filaments. In these models the motility of demembranated cytoplasm can be controlled in vitro, and the chemical conditions necessary for contractility, and cytoplasmic streaming can be investigated. It is clear from these studies that “fibrils” exist in amoeboid models, and that they are capable of contracting along their length under conditions similar to those which cause contraction in vertebrate muscles.

Intracellular trafficking and cytoplasmic streaming under abiotic stress conditions

2019 ◽  
Vol 6 (04) ◽  
Author(s):  
JESHIMA KHAN YASIN ◽  
ANIL KUMAR SINGH
Keyword(s):  
Plasma Membrane ◽  
Abiotic Stress ◽  
Confocal Microscopy ◽  
Fusion Protein ◽  
Intracellular Trafficking ◽  
Cytoplasmic Streaming ◽  
Living Cells ◽  
Stress Conditions ◽  
Vesicle Formation ◽  
External Stimuli

Cytoplasmic streaming is one among the vital activities of the living cells. In plants cytolplasmic streaming could clearly be seen in hypocotyls of growing seedlings. To observe cytoplsmic streaming and its correlated intracellular trafficking an investigation was conducted in legumes in comparison with GFP-AtRab75 and 35S::GFP:δTIP tonoplast fusion protein expressing arabidopsis lines. These seedlings were observed under confocal microscopy with different buffer incubation treatments and under different stress conditions. GFP expressing 35S::GFP:δTIP tonoplast lines were looking similar to the control lines and differ under stress conditions. Movement of cytoplasmic invaginations within the tonoplast and cytoplasmic sub vesicle or bulb budding during cytoplasmic streaming was observed in hypocotyls of At-GFP tonoplast plants. We found the cytoplasmic bulbs/ vesicles or sub vesicle formation from the plasma membrane. The streaming speed also depends on the incubation medium in which the specimen was incubated, indicating that the external stimuli as well as internal stimuli can alter the speed of streaming

scholarly journals Microtubule–microtubule sliding by kinesin-1 is essential for normal cytoplasmic streaming in Drosophila oocytes

2016 ◽  
Vol 113 (34) ◽  
pp. E4995-E5004 ◽  
Author(s):  
Wen Lu ◽  
Michael Winding ◽  
Margot Lakonishok ◽  
Jill Wildonger ◽  
Vladimir I. Gelfand
Keyword(s):  
Cytoplasmic Streaming ◽  
Cell Types ◽  
Nurse Cells ◽  
Wild Type ◽  
Actin Cortex ◽  
Microtubule Motor ◽  
Multiple Cell ◽  
Cytoplasmic Microtubules ◽  
Two Populations

Cytoplasmic streaming in Drosophila oocytes is a microtubule-based bulk cytoplasmic movement. Streaming efficiently circulates and localizes mRNAs and proteins deposited by the nurse cells across the oocyte. This movement is driven by kinesin-1, a major microtubule motor. Recently, we have shown that kinesin-1 heavy chain (KHC) can transport one microtubule on another microtubule, thus driving microtubule–microtubule sliding in multiple cell types. To study the role of microtubule sliding in oocyte cytoplasmic streaming, we used a Khc mutant that is deficient in microtubule sliding but able to transport a majority of cargoes. We demonstrated that streaming is reduced by genomic replacement of wild-type Khc with this sliding-deficient mutant. Streaming can be fully rescued by wild-type KHC and partially rescued by a chimeric motor that cannot move organelles but is active in microtubule sliding. Consistent with these data, we identified two populations of microtubules in fast-streaming oocytes: a network of stable microtubules anchored to the actin cortex and free cytoplasmic microtubules that moved in the ooplasm. We further demonstrated that the reduced streaming in sliding-deficient oocytes resulted in posterior determination defects. Together, we propose that kinesin-1 slides free cytoplasmic microtubules against cortically immobilized microtubules, generating forces that contribute to cytoplasmic streaming and are essential for the refinement of posterior determinants.

scholarly journals Color Response Modification of Encapsulated Liquid Crystals Used in Rotating Disk Heat Transfer Studies

1995 ◽  
Author(s):  
Cengiz Camci ◽  
Boris Glezer
Keyword(s):  
Heat Transfer ◽  
Liquid Crystal ◽  
Liquid Crystals ◽  
Rotational Speed ◽  
Rotating Disk ◽  
Point Of View ◽  
Centrifugal Acceleration ◽  
Relative Shift ◽  
Color Response ◽  
Stationary Conditions

The liquid crystal thermography can be successfully used in both transient and steady-state heat transfer experiments with excellent spatial resolution and good accuracy. Although most of the past liquid crystal based heat transfer studies are reported in the stationary frame, measurements from the rotating frame of turbomachinery systems exist The main objective of the present investigation is to determine the influence of rotation on the color calibration of encapsulated liquid crystals sprayed on the flat surface of a rotating aluminum disk. The investigation is performed for a rotational speed range from 0 rpm to 7500 rpm using three different liquid crystal coatings displaying red at 30, 35 and 45° C, under stationary conditions. An immediate observation from the present study is that the color response of liquid crystals is strongly modified by the centrifugal acceleration of the rotating environment. It is consistently and repeatedly observed that the hue versus temperature curve is continuously shifted toward lower temperatures by increasing rotational speed. The relative shift of the display temperature of the green can be as high as 7°C at 7500 rpm when compared to the temperature of the green observed under stationary conditions. The present study shows that relative shift of the liquid crystal color has a well-defined functional dependency to rotational speed. The shift is linearly proportional to the centrifugal acceleration. It is interesting to note that the individual shift curves of the green for all three liquid crystal coatings collapse into a single curve when they are normalized with respect to their own stationary green values. When the color attribute is selected as “intensity” instead of “hue”, very similar shifts of the temperature corresponding to the intensity maximum value appearing around green is observed. An interpretation of the observed color shift is made from a thermodynamics energy balance point of view.

Photon correlation analysis of cytoplasmic streaming

1976 ◽  
Vol 444 (3) ◽  
pp. 893-898 ◽  
Author(s):  
K.H. Langley ◽  
R.W. Piddington ◽  
D. Ross ◽  
D.B. Sattelle
Keyword(s):  
Correlation Analysis ◽  
Cytoplasmic Streaming ◽  
Photon Correlation

Cytoplasmic streaming in characean cells: role of subcortical fibrils

1980 ◽  
Vol 58 (7) ◽  
pp. 760-765 ◽  
Author(s):  
Eiji Kamitsubo
Keyword(s):  
Electrical Stimulation ◽  
Experimental Evidence ◽  
Cytoplasmic Streaming ◽  
Motive Force ◽  
Microbeam Irradiation ◽  
Subcortical Fibrils

Three or four parallel fibrils of ca. 0.1 μm in width attached to each file of chloroplasts in intact internodal cells generate the motive force for cytoplasmic streaming. Experimental evidence for this conclusion is drawn from experiments in which fibrillar motion and streaming are interrupted by centrifugation, microbeam irradiation, and electrical stimulation. The role of Pb2+ in preventing cessation of cytoplasmic streaming after electrical stimulation is interpreted in terms of localized changes in viscosity of the cytoplasm.

Studies on cessation of cytoplasmic streaming under K+-induced depolarization inNitella axilliformis

1995 ◽  
Vol 108 (4) ◽  
pp. 457-462 ◽  
Author(s):  
Teruo Shimmen ◽  
Munehiro Kikuyama ◽  
Masashi Tazawa
Keyword(s):  
Cytoplasmic Streaming
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