Variation in the susceptibility of BHK populations and cloned cell lines to three strains of foot-and-mouth disease virus

1980 ◽  
Vol 63 (1) ◽  
pp. 1-9 ◽  
Author(s):  
J. B. Clarke ◽  
R. E. Spier
Keyword(s):  
Disease Virus ◽  
Cell Lines ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Foot And Mouth

The carboxy-terminal half of nonstructural protein 3A is not essential for foot-and-mouth disease virus replication in cultured cell lines

2016 ◽  
Vol 161 (5) ◽  
pp. 1295-1305 ◽  
Author(s):  
Mrutyunjay Behura ◽  
Jajati K. Mohapatra ◽  
Laxmi K. Pandey ◽  
Biswajit Das ◽  
Mukesh Bhatt ◽  
...  
Keyword(s):  
Disease Virus ◽  
Cell Lines ◽  
Virus Replication ◽  
Nonstructural Protein ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Cultured Cell ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Carboxy Terminal

scholarly journals Persistence of foot-and-mouth disease virus in cell culture revisited: implications for contingency in evolution

2008 ◽  
Vol 89 (1) ◽  
pp. 232-244 ◽  
Author(s):  
Mónica Herrera ◽  
Ana Grande-Pérez ◽  
Celia Perales ◽  
Esteban Domingo
Keyword(s):  
Cell Culture ◽  
Disease Virus ◽  
Cell Lines ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Persistently Infected ◽  
Established Cell Lines ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Established Cell

If we could rewind the tape of evolution and play it again, would it turn out to be similar to or different from what we know? Obviously, this key question can only be addressed by fragmentary experimental approaches. Twenty-two years ago, we described the establishment of BHK-21 cells persistently infected with foot-and-mouth disease virus (FMDV), a system that displayed as its major biological feature a coevolution of the cells and the resident virus in the course of persistence. Now we report the establishment of two persistently infected cell lines in parallel, starting with the same clones of FMDV and BHK-21 cells used 22 years ago. We have asked whether the evolution of the two newly established cell lines and of the earlier cell line would be similar or different. The main conclusions of the study are: (i) the basic behaviour characterized by virus–cell coevolution is similar in the three carrier cell lines, despite differences in some genetic alterations of FMDV; (ii) a strikingly parallel behaviour has been observed with the two newly established cell lines passaged in parallel, unveiling a deterministic virus behaviour during persistence; and (iii) selective RT-PCR amplifications have detected imbalances in the proportion of positive- versus negative-strand viral RNA, mediated by both viral and cellular factors. The results confirm coevolution of cells and virus as a major and reproducible feature of FMDV persistence in cell culture, and suggest that rapidly evolving viruses may constitute adequate test systems to probe the influence of historical contingency on evolutionary events.

scholarly journals Antiviral efficacy of short-hairpin RNAs and artificial microRNAs targeting foot-and-mouth disease virus

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11227
Author(s):  
Anabella Currá ◽  
Marco Cacciabue ◽  
María José Gravisaco ◽  
Sebastián Asurmendi ◽  
Oscar Taboga ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Disease Virus ◽  
Cell Lines ◽  
Small Rna ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Short Hairpin ◽  
Short Hairpin Rnas

RNA interference (RNAi) is a well-conserved mechanism in eukaryotic cells that directs post-transcriptional gene silencing through small RNA molecules. RNAi has been proposed as an alternative approach for rapid and specific control of viruses including foot-and-mouth disease virus (FMDV), the causative agent of a devastating animal disease with high economic impact. The aim of this work was to assess the antiviral activity of different small RNA shuttles targeting the FMDV RNA-dependent RNA polymerase coding sequence (3D). Three target sequences were predicted within 3D considering RNA accessibility as a major criterion. The silencing efficacy of short-hairpin RNAs (shRNAs) and artificial microRNAs (amiRNAs) targeting the selected sequences was confirmed in fluorescent reporter assays. Furthermore, BHK-21 cells transiently expressing shRNAs or amiRNAs proved 70 to >95% inhibition of FMDV growth. Interestingly, dual expression of amiRNAs did not improve FMDV silencing. Lastly, stable cell lines constitutively expressing amiRNAs were established and characterized in terms of antiviral activity against FMDV. As expected, viral replication in these cell lines was delayed. These results show that the target RNA-accessibility-guided approach for RNAi design rendered efficient amiRNAs that constrain FMDV replication. The application of amiRNAs to complement FMDV vaccination in specific epidemiological scenarios shall be explored further.

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