Biochemical studies with infectious bursal disease virus: Comparison of some of its properties with infectious pancreatic necrosis virus

1979 ◽  
Vol 60 (3-4) ◽  
pp. 265-277 ◽  
Author(s):  
D. Todd ◽  
M. S. McNulty
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Pancreatic Necrosis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Infectious Bursal Disease ◽  
Necrosis Virus ◽  
Biochemical Studies ◽  
Bursal Disease ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

Immunofluorescent detection of double-stranded RNA in cells infected with reovirus, infectious pancreatic necrosis virus, and infectious bursal disease virus

1980 ◽  
Vol 26 (2) ◽  
pp. 256-261 ◽  
Author(s):  
Richard D. Macdonald
Keyword(s):  
Infectious Bursal Disease Virus ◽  
Pancreatic Necrosis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Necrosis Virus ◽  
Urea Treatment ◽  
Double Stranded Rna ◽  
Bursal Disease ◽  
Infected Cells ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

Urea treatment of ethanol-fixed virus-infected cells exposed nucleic acid antigens for immunofluorescence. Three double-stranded (ds) RNA-containing viruses showed bright fluorescence using antibodies against dsRNA. Three single-stranded RNA-containing viruses showed less intense fluorescence with anti-dsRNA. Four out of five cell lines persistently infected with various RNA-containing viruses showed no dsRNA detectable by immunofluorescence.

scholarly journals Peptides resulting from the pVP2 C-terminal processing are present in infectious pancreatic necrosis virus particles

2004 ◽  
Vol 85 (8) ◽  
pp. 2231-2236 ◽  
Author(s):  
Marie Galloux ◽  
Christophe Chevalier ◽  
Celine Henry ◽  
Jean-Claude Huet ◽  
Bruno Da Costa ◽  
...  
Keyword(s):  
Pancreatic Necrosis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Necrosis Virus ◽  
Virus Particles ◽  
C Terminus ◽  
Proteolytic Cascade ◽  
Final Maturation ◽  
Bursal Disease ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

The capsid of birnaviruses contains two proteins, VP2 and VP3, which derive from the processing of a large polyprotein, NH2–pVP2–VP4–VP3–COOH. The proteolytic cascade involved in processing the polyprotein, and in the final maturation of pVP2 (the precursor of VP2), has recently been shown to generate VP2 and four structural peptides in infectious bursal disease virus and blotched snakehead virus. The presence of peptides in infectious pancreatic necrosis virus particles was investigated using mass spectrometry and N-terminal sequencing of virus particles. Three peptides deriving from the C terminus of pVP2 (residues 443–486, 487–495 and 496–508 of the polyprotein) and 14 additional peptides produced by further processing of peptides [443–486] and [496–508] were identified. These results indicate that the presence of several virus-encoded peptides in the virions is a hallmark of birnaviruses.

scholarly journals Restriction Fragment Length Polymorphisms and Sequence Analysis: an Approach for Genotyping Infectious Pancreatic Necrosis Virus Reference Strains and Other Aquabirnaviruses Isolated from Northwestern Spain

2004 ◽  
Vol 70 (2) ◽  
pp. 1059-1067 ◽  
Author(s):  
J. M. Cutrín ◽  
J. L. Barja ◽  
B. L. Nicholson ◽  
I. Bandín ◽  
S. Blake ◽  
...  
Keyword(s):  
Pancreatic Necrosis ◽  
Restriction Analysis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Necrosis Virus ◽  
Genotype I ◽  
Northwestern Spain ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus ◽  
Reference Strains ◽  
Restriction Fragment Length

ABSTRACT Reference strains of infectious pancreatic necrosis virus resembling the 10 recognized serotypes and local isolates of aquabirnaviruses isolated in northwestern Spain from reservoirs (mollusks) and from asymptomatic and carrier cultured fish were genotyped by restriction fragment length polymorphism (RFLP) and nucleic acid sequence analyses. The RFLP analysis yielded seven genogroups, each of which was clearly correlated with a serotype. Sequence analysis of the three open reading frames provided quite similar results in terms of genogrouping. Based on the results of this study and in order to unify the two types of assays, we propose placing aquabirnaviruses into six genogroups, four of which can be subdivided into two genotypes based on a two-step restriction analysis. The genotyping corresponds with serotyping as follows: genogroup I includes two genotypes corresponding to serotypes A9 (genotype I.1) and A1 (genotype I.2); genogroup II corresponds to serotype A3; genogroup III includes genotypes III.1 (serotype A2) and III.2 (serotype B1); genogroups IV and V include two genotypes, each corresponding to serotypes A5, A6, A7, and A8 (genotypes IV.1, IV.2, V.1, and V.2, respectively);and genogroup VI corresponds to serotype A4. As expected, most local isolates belonged to genotype III.1 and genogroup II. However, a few local isolates corresponded to the American types of genogroup I. Finally, based on the results of this study and due to its simplicity, the two-step restriction analysis assay is proposed as a method for typing new isolates of aquabirnaviruses, and the results correspond to the results of conventional serotyping.

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