Use of egg-yolk antibody for detection of respiratory syncytial virus in nasal secretions by ELISA

1986 ◽  
Vol 90 (3-4) ◽  
pp. 197-206 ◽  
Author(s):  
M. Zrein ◽  
G. Obert ◽  
M. H. V. van Regenmortel
Keyword(s):  
Respiratory Syncytial Virus ◽  
Egg Yolk ◽  
Nasal Secretions ◽  
Syncytial Virus ◽  
Egg Yolk Antibody

Beta-Chemokines in Nasal Secretions of Infants with Respiratory Syncytial Virus-Induced Respiratory Infections

2001 ◽  
Vol 15 (2) ◽  
pp. 89-96 ◽  
Author(s):  
Roberto P. Garofalo ◽  
Barbara Olszewska-Pazdrak ◽  
Pearay L. Ogra ◽  
Robert C. Welliver
Keyword(s):  
Respiratory Syncytial Virus ◽  
Respiratory Infections ◽  
Nasal Secretions ◽  
Syncytial Virus

scholarly journals Class-specific antibodies to bovine respiratory syncytial virus in experimentally infected lambs

1992 ◽  
Vol 108 (1) ◽  
pp. 135-145 ◽  
Author(s):  
R. Sharma ◽  
Z. Woldehiwet
Keyword(s):  
Respiratory Syncytial Virus ◽  
Sandwich Elisa ◽  
Lung Lavage ◽  
Bovine Respiratory Syncytial Virus ◽  
Post Inoculation ◽  
Serum Samples ◽  
Iga Antibodies ◽  
Nasal Secretions ◽  
Specific Igg ◽  
Syncytial Virus

SUMMARYEnzyme-linked immunoabsorbent assay (ELISA) was used to titrate virus-specific IgG, IgM and IgA levels in nasal secretions, lung lavage fluids and serum samples sequentially obtained from lambs experimentally infected with bovine respiratory syncytial virus (RSV). Virus-specific IgG and IgM responses were measured by the indirect double antibody sandwich ELISA using anti-bovine RSV monoclonal antibody, as capture antibody, and peroxidase-conjugated anti-sheep IgG and anti-sheep IgM. Virus-specific IgA antibodies were measured by antibody capture assay using anti-sheep IgA (α–chain specific) and anti-bovine RSV monoclonal antibodies.Bovine RSV-specific IgM and IgA antibodies were detected in the serum samples within 6 days post-inoculation (p.i.). Virus-specific IgC antibodies appeared in serum samples 4 days later. In nasal secretions, IgA antibodies appeared 7 days p.i. but IgM antibodies were not detected until 12–16 days p.i. In serum samples, IgM titres were predominant for the first 2 weeks p.i. IgC titres becoming predominant thereafter. In nasal secretions and lung lavage fluids, IgA titres were significantly higher than IgM or IgG titres up to 21 days p.i. (0·01).

Respiratory Syncytial Virus Neutralizing Activity in Nasal Secretions Following Natural Infection

1969 ◽  
Vol 131 (2) ◽  
pp. 658-661 ◽  
Author(s):  
H. W. Kim ◽  
J. A. Bellanti ◽  
J. O. Arrobio ◽  
J. Mills ◽  
C. D. Brandt ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Natural Infection ◽  
Neutralizing Activity ◽  
Nasal Secretions ◽  
Syncytial Virus

scholarly journals Quantification of Respiratory Syncytial Virus Polypeptides in Nasal Secretions by Monoclonal Antibodies

1985 ◽  
Vol 66 (8) ◽  
pp. 1705-1714 ◽  
Author(s):  
R. M. Hendry ◽  
E. Godfrey ◽  
L. J. Anderson ◽  
B. F. Fernie ◽  
K. McIntosh
Keyword(s):  
Monoclonal Antibodies ◽  
Respiratory Syncytial Virus ◽  
Nasal Secretions ◽  
Syncytial Virus
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