An electron microscopic study of MDBK cells persistently infected with Newcastle disease virus

1977 ◽  
Vol 53 (3) ◽  
pp. 185-195 ◽  
Author(s):  
M. S. McNulty ◽  
E. J. Gowans ◽  
A. C. Louz� ◽  
G. Fraser
Keyword(s):  
Newcastle Disease Virus ◽  
Microscopic Study ◽  
Disease Virus ◽  
Electron Microscopic Study ◽  
Newcastle Disease ◽  
Electron Microscopic ◽  
Persistently Infected ◽  
Mdbk Cells

Electron Microscopic Study of Penetration of Newcastle Disease Virus into Cells Leading to Formation of Polykaryocytes

1967 ◽  
Vol 2 (1) ◽  
pp. 71-76
Author(s):  
N. MEISELMAN ◽  
A. KOHN ◽  
D. DANON
Keyword(s):  
Epithelial Cells ◽  
Cell Membrane ◽  
Newcastle Disease Virus ◽  
Microscopic Study ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Cell Membranes ◽  
Viral Envelope ◽  
Electron Microscopic ◽  
Input Multiplicity

Treatment of FL or Lu 106 epithelial cells with Newcastle disease virus (NDV) at an input multiplicity of 500 EID50 per cell induces in these cells the formation of polykaryocytes at the end of 2-3 h of contact. Electron micrographs of such NDV-treated monolayers after 2-10 min of incubation show the presence of virions adsorbed to the cell membranes, in vacuoles and with the viral envelope partly fused with the cell membrane. In polykaryocytes induced by NDV, remnants of cell membranes showing numerous breaks may still be present after 3 h.

scholarly journals Effect of Exogenous Interferon on L Cells Persistently Infected with Newcastle Disease Virus

1972 ◽  
Vol 5 (1) ◽  
pp. 145-146 ◽  
Author(s):  
Jules V. Hallum ◽  
Harshad R. Thacore ◽  
Julius S. Youngner
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Persistently Infected ◽  
L Cells

scholarly journals Analysis of PPI networks of transcriptomic expression identifies hub genes associated with Newcastle disease virus persistent infection in bladder cancer

2020 ◽  
Author(s):  
Umar Ahmad ◽  
De Ming Chau ◽  
Suet Lin Chia ◽  
Khatijah Yusoff ◽  
Syahril Abdullah ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Newcastle Disease Virus ◽  
Cancer Cells ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Persistent Infection ◽  
Ppi Network ◽  
Hub Genes ◽  
Persistently Infected ◽  
Bladder Cancer Cells

AbstractMotivationBladder cancer cells acquire persistent infection associated with oncolytic Newcastle disease virus (NDV) in which its molecular events are still unclear. This poses a potential problem for oncolytic virus application for cancer therapy. To unravel the molecular mechanism underlying the development of NDV persistent infection in bladder cancer, we used mRNA expression profile of the persistently infected bladder cancer cells to construct PPI network.ResultsBased on path and module exploring in the PPI network, the bridges were found mainly from pathways of p53 signalling, ECM-receptor interaction, and TGF-beta signalling by the upregulated mRNAs, to the antigen processing and presentation, protein processing in endoplasmic reticulum, completement and coagulation cascades by the downregulated mRNAs in NDV persistent TCCSUPPi cells. In persistent EJ28Pi cells comparatively, connections were identified mainly from pathways of renal carcinoma, viral carcinogenesis, Ras signalling and cell cycle by the upregulated mRNAs, to the Wnt signalling, HTLV-I infection and pathways in cancer by the downregulated mRNAs. This connection was mainly dependent on of RPL8- HSPA1A/HSPA4 in TCCSUPPi cells and EP300, PTPN11, RAC1 - TP53, SP1, CCND1 and XPO1 in EJ28Pi cells. Oncomine validation showed that the top hub genes identified in the network that includes RPL8, THBS1, F2 from TCCSUPPi and TP53 and RAC1 from EJ28Pi are involved in the development and progression of bladder cancer. Protein-drug interaction network, have identified several drugs targets that could be used to disconnect the linkages between modules and prevent bladder cancer cells from acquiring NDV persistent infection. This is the first time reporting the PPI network analysis of differentially expressed mRNAs of the NDV persistently infected bladder cancer cell lines which provide an insight into screening drugs that could be used together with NDV to manage bladder cancer resistance to therapy and progression.

scholarly journals Cells Persistently Infected with Newcastle Disease Virus

1970 ◽  
Vol 6 (1) ◽  
pp. 42-48 ◽  
Author(s):  
Harshad Thacore ◽  
Julius S. Youngner
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Persistently Infected
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