Dye-induced fluorescence of tumour cells: photochemical action spectra

1974 ◽  
Vol 6 (3) ◽  
pp. 237-243 ◽  
Author(s):  
A. L. Bastos ◽  
D. Marques ◽  
M. A. Affra
Keyword(s):  
Tumour Cells ◽  
Action Spectra ◽  
Photochemical Action

scholarly journals Photochemical action spectra indicate that cytochrome a/a3 is the predominant haemoprotein terminal oxidase in Acanthamoeba castellanii

1983 ◽  
Vol 210 (3) ◽  
pp. 721-725 ◽  
Author(s):  
R I Scott ◽  
D Lloyd
Keyword(s):  
Cytochrome B ◽  
Room Temperature ◽  
Stationary Phases ◽  
Acanthamoeba Castellanii ◽  
Terminal Oxidase ◽  
Difference Spectra ◽  
Intact Cells ◽  
Action Spectra ◽  
Stages Of Growth ◽  
Photochemical Action

1. Room-temperature CO-reduced minus reduced difference spectra of intact cells of Acanthamoeba castellanii show the presence of CO-reacting haemoproteins in cells from the early-exponential, late-exponential and stationary phases of growth. 2. The relative rates of reaction with CO of the two haemoproteins differ; that of cytochrome a/a3 with CO is complete within 1 min of bubbling with CO, whereas that of cytochrome b takes longer than 90 min. 3. Photochemical action spectra reveal cytochrome a/a3 as the predominant haemoprotein oxidase at all stages of growth. 4. It is concluded that the alternative oxidases known to be present in these organisms are not cytochromes.

scholarly journals Haemoprotein terminal oxidases in the nematodes Nippostrongylus brasiliensis and Ascaridia galli

1988 ◽  
Vol 256 (1) ◽  
pp. 295-298 ◽  
Author(s):  
T A Paget ◽  
M Fry ◽  
D Lloyd
Keyword(s):  
Muscle Tissue ◽  
Endogenous Respiration ◽  
Nippostrongylus Brasiliensis ◽  
Reproductive Tissue ◽  
Ascaridia Galli ◽  
Difference Spectra ◽  
Cytochrome Aa3 ◽  
Action Spectra ◽  
Terminal Oxidases ◽  
Photochemical Action

1. Mitochondria isolated from the gut-dwelling nematodes Nippostrongylus brasiliensis and Ascaridia galli (muscle and gut + reproductive tissue) were examined for cytochromes, and it was observed that N. brasiliensis and A. galli muscle tissue mitochondria contained a-, b- and c-type cytochromes, but their stoichiometries were quite different (1:2:1.9 and 1:11.4:13.6 respectively); A. galli gut + reproductive-tissue mitochondria, however, only contained b and c cytochromes, in a ratio of 1:0.8. 2. CO difference spectra showed the presence of CO-reacting b-type cytochrome(s) in all three types of mitochondria; the fast-reacting species comprised 30, 44 and 39% of the total in N. brasiliensis, A. galli muscle and A. galli gut + reproductive-tissue mitochondria respectively. 3. Cytochrome aa3 was observed in N. brasiliensis mitochondria and in those from A. galli muscle, but was below the level of detectability (less than 0.005 nmol/mg of protein) for A. galli gut + reproductive-tissue mitochondria. 4. Photochemical action spectra for the reversal of CO inhibition of the endogenous respiration of whole worms (at 24 microM- and 40 microM-O2 respectively for N. brasiliensis and A. galli) gave maxima at 598 and 542-543 nm, corresponding to the alpha- and beta-absorption maxima of cytochrome aa3, and at 567 nm (b-type cytochrome) for both worms. These results suggest that cytochrome aa3 is the major functional oxidase in N. brasiliensis, whereas the CO-reacting b-type cytochrome dominates in A. galli.

scholarly journals Studies of Photochemical Action Spectra on N, N, N', N'-Tetramethyl-p-phenylenediamine-Oxidase-Negative Mutants of Azotobacter vinelandii

1980 ◽  
Vol 105 (1) ◽  
pp. 177-185 ◽  
Author(s):  
Paul S. HOFFMAN ◽  
Richard M. IRWIN ◽  
L. A. CARREIRA ◽  
T. Vance MORGAN ◽  
Burt D. ENSLEY ◽  
...  
Keyword(s):  
Azotobacter Vinelandii ◽  
Action Spectra ◽  
Photochemical Action
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