A mouse attenuated mutant of Junin virus with an altered envelope glycoprotein

1990 ◽  
Vol 111 (3-4) ◽  
pp. 257-262 ◽  
Author(s):  
L. A. Scolaro ◽  
Susana E. Mersich ◽  
Elsa B. Damonte
Keyword(s):  
Envelope Glycoprotein ◽  
Junin Virus ◽  
Junín Virus

scholarly journals Structure of a Zinc-binding Domain in the Junín Virus Envelope Glycoprotein

2010 ◽  
Vol 286 (2) ◽  
pp. 1528-1536 ◽  
Author(s):  
Klára Briknarová ◽  
Celestine J. Thomas ◽  
Joanne York ◽  
Jack H. Nunberg
Keyword(s):  
Envelope Glycoprotein ◽  
Binding Domain ◽  
Zinc Binding ◽  
Virus Envelope ◽  
Junin Virus ◽  
Zinc Binding Domain ◽  
Junín Virus

scholarly journals Epistastic Interactions within the Junín Virus Envelope Glycoprotein Complex Provide an Evolutionary Barrier to Reversion in the Live-Attenuated Candid#1 Vaccine

2017 ◽  
Vol 92 (1) ◽  
Author(s):  
Joanne York ◽  
Jack H. Nunberg
Keyword(s):  
Envelope Glycoprotein ◽  
Epistatic Interaction ◽  
Second Generation ◽  
Hemorrhagic Fever ◽  
Viral Envelope ◽  
Junin Virus ◽  
Glycoprotein Complex ◽  
Viral Envelope Glycoprotein ◽  
Argentine Hemorrhagic Fever ◽  
Junín Virus

ABSTRACTThe Candid#1 strain of Junín virus was developed using a conventional attenuation strategy of serial passage in nonhost animals and cultured cells. The live-attenuated Candid#1 vaccine is used in Argentina to protect at-risk individuals against Argentine hemorrhagic fever, but it has not been licensed in the United States. Recent studies have revealed that Candid#1 attenuation is entirely dependent on a phenylalanine-to-isoleucine substitution at position 427 in the fusion subunit (GP2) of the viral envelope glycoprotein complex (GPC), thereby raising concerns regarding the potential for reversion to virulence. In this study, we report the identification and characterization of an intragenic epistatic interaction between the attenuating F427I mutation in GP2 and a lysine-to-serine mutation at position 33 in the stable signal peptide (SSP) subunit of GPC, and we demonstrate the utility of this interaction in creating an evolutionary barrier against reversion to the pathogenic genotype. In the presence of the wild-type F427 residue, the K33S mutation abrogates the ability of ectopically expressed GPC to mediate membrane fusion at endosomal pH. This defect is rescued by the attenuating F427I mutation. We show that the recombinant Candid#1 (rCan) virus bearing K33S GPC is viable and retains its attenuated genotype under cell culture conditions that readily select for reversion in the parental rCan virus. If back-mutation to F427 offers an accessible pathway to increase fitness in rCan, reversion in K33S-GPC rCan is likely to be lethal. The epistatic interaction between K33S and F427I thus may minimize the likelihood of reversion and enhance safety in a second-generation Candid#1 vaccine.IMPORTANCEThe live-attenuated Candid#1 vaccine strain of Junín virus is used to protect against Argentine hemorrhagic fever. Recent findings that a single missense mutation in the viral envelope glycoprotein complex (GPC) is responsible for attenuation raise the prospect of facile reversion to pathogenicity. Here, we characterize a genetic interaction between GPC subunits that evolutionarily forces retention of the attenuating mutation. By incorporating this secondary mutation into Candid#1 GPC, we hope to minimize the likelihood of reversion and enhance safety in a second-generation Candid#1 vaccine. A similar approach may guide the design of live-attenuated vaccines against Lassa and other arenaviral hemorrhagic fevers.

Junin virus activity in two rural populations of the Argentine hemorrhagic fever (AHF) endemic area

1983 ◽  
Vol 12 (4) ◽  
pp. 273-280 ◽  
Author(s):  
Mercedes C. Weissenbacher ◽  
Marta S. Sabattini ◽  
María M. Avila ◽  
Patricia M. Sangiorgio ◽  
María R. F. De Sensi ◽  
...  
Keyword(s):  
Endemic Area ◽  
Hemorrhagic Fever ◽  
Rural Populations ◽  
Junin Virus ◽  
Argentine Hemorrhagic Fever ◽  
Virus Activity ◽  
Junín Virus

A Rapid Method for Detecting Junin Virus Viremia in the Guinea Pig

Intervirology ◽  
1977 ◽  
Vol 8 (6) ◽  
pp. 360-363 ◽  
Author(s):  
Adriana Rabinovich ◽  
Patricio M. Cossio ◽  
Guadalupe Carballal ◽  
Roberto M. Arana
Keyword(s):  
Guinea Pig ◽  
Rapid Method ◽  
Junin Virus ◽  
Junín Virus

scholarly journals Development of Peptide-Conjugated Morpholino Oligomers as Pan-Arenavirus Inhibitors

2011 ◽  
Vol 55 (10) ◽  
pp. 4631-4638 ◽  
Author(s):  
Benjamin W. Neuman ◽  
Lydia H. Bederka ◽  
David A. Stein ◽  
Joey P. C. Ting ◽  
Hong M. Moulton ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Treatment Options ◽  
Hemorrhagic Fever ◽  
Virus Genome ◽  
Lymphocytic Choriomeningitis ◽  
Content Type ◽  
Junin Virus ◽  
Pichinde Virus ◽  
Junín Virus

ABSTRACTMembers of theArenaviridaefamily are a threat to public health and can cause meningitis and hemorrhagic fever, and yet treatment options remain limited by a lack of effective antivirals. In this study, we found that peptide-conjugated phosphorodiamidate morpholino oligomers (PPMO) complementary to viral genomic RNA were effective in reducing arenavirus replication in cell cultures andin vivo. PPMO complementary to the Junín virus genome were designed to interfere with viral RNA synthesis or translation or both. However, only PPMO designed to potentially interfere with translation were effective in reducing virus replication. PPMO complementary to sequences that are highly conserved across the arenaviruses and located at the 5′ termini of both genomic segments were effective against Junín virus, Tacaribe virus, Pichinde virus, and lymphocytic choriomeningitis virus (LCMV)-infected cell cultures and suppressed viral titers in the livers of LCMV-infected mice. These results suggest that arenavirus 5′ genomic termini represent promising targets for pan-arenavirus antiviral therapeutic development.

Effect of Ribavirin on Junin Virus Infection in Guinea Pigs

2012 ◽  
Vol 59 (4) ◽  
pp. 278-285 ◽  
Author(s):  
M. Salazar ◽  
N. E. Yun ◽  
A. L. Poussard ◽  
J. N. Smith ◽  
J. K. Smith ◽  
...  
Keyword(s):  
Virus Infection ◽  
Guinea Pigs ◽  
Junin Virus ◽  
Junín Virus
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