Proviral sequences detected by polymerase chain reaction in peripheral blood cells of horses with equine infectious anemia lentivirus

1991 ◽  
Vol 117 (1-2) ◽  
pp. 109-119 ◽  
Author(s):  
Katherine I. O'Rourke ◽  
M. L. Besola ◽  
T. C. McGuire
Keyword(s):  
Polymerase Chain Reaction ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Peripheral Blood Cells ◽  
Chain Reaction ◽  
Equine Infectious Anemia ◽  
Polymerase Chain

scholarly journals New sensitive method for the detection of the A3243G mutation of human mitochondrial deoxyribonucleic acid in diabetes mellitus patients by ligation-mediated polymerase chain reaction

1998 ◽  
Vol 44 (10) ◽  
pp. 2088-2093 ◽  
Author(s):  
Michiyo Urata ◽  
Machiko Wakiyama ◽  
Masanori Iwase ◽  
Makoto Yoneda ◽  
Sachiko Kinoshita ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Polymerase Chain Reaction ◽  
Conventional Method ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Nucleotide Position ◽  
Peripheral Blood Cells ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Sensitive Method

Abstract An adenine-to-guanine mutation at nucleotide position (np) 3243 in the mitochondrial tRNALeu(UUR) gene is closely associated with various clinical phenotypes of diabetes mellitus. Because the mutation creates a new restriction site for the restriction enzyme ApaI, the mutation is usually detected and quantified by ApaI cleavage of the PCR products including np 3243. The sensitivity of the conventional method is, however, 5–10% heteroplasmy. The percentage of heteroplasmy of the mutation is usually highest in the affected tissues and is much lower in peripheral blood cells, which are used most frequently for the analysis. The sensitivity of the conventional method, however, is not sufficient to detect the mutation from peripheral blood cells. Utilizing ligation-mediated polymerase chain reaction, we have developed a feasible and sensitive method to detect 0.01% heteroplasmy of the 3243 mutation in peripheral leukocytes.

scholarly journals Peripheral blood cells are predominantly chimeric of affected and normal cells in patients with paroxysmal nocturnal hemoglobinuria: simultaneous investigation on clonality and expression of glycophosphatidylinositol-anchored proteins

Blood ◽  
1994 ◽  
Vol 83 (3) ◽  
pp. 853-859 ◽  
Author(s):  
H Ohashi ◽  
T Hotta ◽  
A Ichikawa ◽  
T Kinoshita ◽  
R Taguchi ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Paroxysmal Nocturnal Hemoglobinuria ◽  
Southern Hybridization ◽  
Peripheral Blood Cells ◽  
Chain Reaction ◽  
Normal Cells ◽  
Clonality Analysis ◽  
Polymerase Chain

To investigate clonal compositions of hematologic cells in paroxysmal nocturnal hemoglobinuria (PNH), we analyzed peripheral blood (PB) cells of 12 female patients with PNH, by clonality analysis using X-chromosome inactivation and assessment of expression of glycophosphatidylinositol-anchored proteins (GPI-APs) by flow cytometry. Southern hybridization showed that granulocytes were monoclonal in three and polyclonal in eight patients, respectively, whereas lymphocytes were polyclonal in all nine patients examined. Expressions of CD16 and CD59 on granulocytes varied greatly in seven patients examined. Clonality analysis of granulocytes by the polymerase chain reaction showed that CD59-and CD59low+ cells were monoclonal, whereas CD59+ cells were polyclonal. It was shown that PB cells are predominantly chimeric of clonal (GPI-AP-or GPI-APlow+) and nonclonal (GPI-AP+) cells in PNH, and that degrees of chimerism differ greatly from patient to patient.

scholarly journals Peripheral blood cells are predominantly chimeric of affected and normal cells in patients with paroxysmal nocturnal hemoglobinuria: simultaneous investigation on clonality and expression of glycophosphatidylinositol-anchored proteins

Blood ◽  
1994 ◽  
Vol 83 (3) ◽  
pp. 853-859 ◽  
Author(s):  
H Ohashi ◽  
T Hotta ◽  
A Ichikawa ◽  
T Kinoshita ◽  
R Taguchi ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Paroxysmal Nocturnal Hemoglobinuria ◽  
Southern Hybridization ◽  
Peripheral Blood Cells ◽  
Chain Reaction ◽  
Normal Cells ◽  
Clonality Analysis ◽  
Polymerase Chain

Abstract To investigate clonal compositions of hematologic cells in paroxysmal nocturnal hemoglobinuria (PNH), we analyzed peripheral blood (PB) cells of 12 female patients with PNH, by clonality analysis using X-chromosome inactivation and assessment of expression of glycophosphatidylinositol-anchored proteins (GPI-APs) by flow cytometry. Southern hybridization showed that granulocytes were monoclonal in three and polyclonal in eight patients, respectively, whereas lymphocytes were polyclonal in all nine patients examined. Expressions of CD16 and CD59 on granulocytes varied greatly in seven patients examined. Clonality analysis of granulocytes by the polymerase chain reaction showed that CD59-and CD59low+ cells were monoclonal, whereas CD59+ cells were polyclonal. It was shown that PB cells are predominantly chimeric of clonal (GPI-AP-or GPI-APlow+) and nonclonal (GPI-AP+) cells in PNH, and that degrees of chimerism differ greatly from patient to patient.

Detection of human cytomegalovirus DNA in peripheral blood leukocytes by the polymerase chain reaction

Transfusion ◽  
2003 ◽  
Vol 33 (9) ◽  
pp. 783-784 ◽  
Author(s):  
Ian.S. Bevan ◽  
Matthew R. Walker ◽  
Richard A. Daw ◽  
Andreas Bitsch ◽  
Gregor Bein ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Human Cytomegalovirus ◽  
Peripheral Blood ◽  
Peripheral Blood Leukocytes ◽  
Chain Reaction ◽  
Blood Leukocytes ◽  
Polymerase Chain
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