IgG avidity assay for estimation of the time after onset of hantavirus infection in colonized and wild bank voles

1993 ◽  
Vol 132 (3-4) ◽  
pp. 359-367 ◽  
Author(s):  
Irina Gavrilovskaya ◽  
N. Apekina ◽  
N. Okulova ◽  
V. Demina ◽  
A. Bernshtein ◽  
...  
Keyword(s):  
Hantavirus Infection ◽  
Avidity Assay ◽  
Bank Voles ◽  
Igg Avidity

scholarly journals Measles Virus IgG Avidity Assay for Use in Classification of Measles Vaccine Failure in Measles Elimination Settings

2012 ◽  
Vol 19 (11) ◽  
pp. 1810-1817 ◽  
Author(s):  
Sara Mercader ◽  
Philip Garcia ◽  
William J. Bellini
Keyword(s):  
Measles Virus ◽  
Area Under The Curve ◽  
Epidemiological Data ◽  
Diagnostic Tools ◽  
High Avidity ◽  
Characteristic Analysis ◽  
Vaccine Failure ◽  
Avidity Assay ◽  
Igg Avidity

ABSTRACTIn regions where endemic measles virus has been eliminated, diagnostic assays are needed to assist in correctly classifying measles cases irrespective of vaccination status. A measles IgG avidity assay was configured using a commercially available measles-specific IgG enzyme immunoassay by modifying the protocol to include three 5-min washes with diethylamine (60 mM; pH 10.25) following serum incubation; serum was serially diluted, and the results were expressed as the end titer avidity index. Receiver operating characteristic analysis was used for evaluation and validation and to establish low (≤30%) and high (≥70%) end titer avidity thresholds. Analysis of 319 serum specimens expected to contain either high- or low-avidity antibodies according to clinical and epidemiological data indicated that the assay is highly accurate, with an area under the curve of 0.998 (95% confidence interval [CI], 0.978 to 1.000), sensitivity of 91.9% (95% CI, 83.2% to 97.0%), and specificity of 98.4% (95% CI, 91.6% to 100%). The assay is rapid (<2 h) and precise (standard deviation [SD], 4% to 7%). In 18 samples from an elimination setting outbreak, the assay identified 2 acute measles cases with low-avidity results; both were IgM-positive samples. Additionally, 11 patients (15 samples) with modified measles who were found to have high-avidity IgG results were classified as secondary vaccine failures; one sample with an intermediate-avidity result was not interpretable. In elimination settings, measles IgG avidity assays can complement existing diagnostic tools in confirming unvaccinated acute cases and, in conjunction with adequate clinical and epidemiologic investigation, aid in the classification of vaccine failure cases.

scholarly journals Demographic Factors Associated with Hantavirus Infection in Bank Voles (Clethrionomys glareolus)

2002 ◽  
Vol 8 (9) ◽  
pp. 924-929 ◽  
Author(s):  
Gert E. Olsson ◽  
Neil White ◽  
Clas Ahlm ◽  
Fredrik Elgh ◽  
Ann-Christin Verlemyr ◽  
...  
Keyword(s):  
Demographic Factors ◽  
Clethrionomys Glareolus ◽  
Hantavirus Infection ◽  
Factors Associated ◽  
Bank Voles

Assessment of IgM enzyme immunoassay and IgG avidity assay for distinguishing between primary and secondary immune response to rubella vaccine

2005 ◽  
Vol 130 (1-2) ◽  
pp. 59-65 ◽  
Author(s):  
Rasool Hamkar ◽  
Somayeh Jalilvand ◽  
Talat Mokhtari-Azad ◽  
Keramat Nouri Jelyani ◽  
Hosein Dahi-Far ◽  
...  
Keyword(s):  
Immune Response ◽  
Enzyme Immunoassay ◽  
Secondary Immune Response ◽  
Rubella Vaccine ◽  
Avidity Assay ◽  
Igg Avidity

scholarly journals Longitudinal Study of SARS-CoV-2 Antibody Characteristics Using Label-Free Immunoassays

2021 ◽  
Vol 156 (Supplement_1) ◽  
pp. S32-S32
Author(s):  
Y R Luo ◽  
C Yun ◽  
A H Wu ◽  
K L Lynch ◽  
I Chakraborty
Keyword(s):  
Symptom Onset ◽  
Neutralizing Antibody ◽  
Label Free ◽  
Serum Samples ◽  
Neutralization Activity ◽  
Antibody Avidity ◽  
Avidity Assay ◽  
Initial Rise ◽  
Igg Avidity ◽  
Antibody Titers

Abstract Introduction/Objective Since the start of the COVID-19 pandemic, much research has focused on the kinetics and magnitude of humoral immune response. With the advantages of monitoring real-time immunoreactions, label-free immunoassay (LFIA) is becoming a powerful tool in serology studies. We have developed LFIAs to measure SARS- CoV-2 antibody avidity and neutralization activity in a cohort of COVID-19 patients and determine if they correlate with antibody concentration. Serial serum samples collected from mild to severe COVID-19 patients were measured out to 8 months post-symptom onset to determine the durability of the neutralizing antibody response. Methods/Case Report Based on thin-film interferometry technology, we established a label-free IgG avidity assay and a label-free surrogate virus neutralization test (LF-sVNT). For measurement, sensing probes pre-coated with receptor-binding domain (RBD) of SARS-CoV-2 spike protein are applied to serum samples containing SARS-CoV-2 antibodies. The label-free IgG avidity assay measures the binding strength between RBD and IgG under urea dissociation. The LF-sVNT analyzes the binding ability of RBD to ACE2 after neutralizing RBD with antibodies. Results (if a Case Study enter NA) IgG avidity indices and neutralizing antibody titers (IC50) were determined from serum samples (n=246) from COVID-19 patients (n=113). IgG concentrations were measured using a fluorescent immunoassay. The neutralizing antibody titers showed a weak correlation with IgG concentrations and no correlation with IgG avidity indices. Over the time course up to 8 months post-symptom onset, IgG concentrations and neutralizing antibody titers presented similar trends: an initial rise, plateau and then in some cases a gradual decline after 40 days. The IgG avidity indices, in the same cases, plateaued after the initial rise. Conclusion The results demonstrated that LFIA could be used an excellent solution in the determination of SARS- CoV-2 antibody characteristics. The study found that IgG concentration and neutralizing antibody titer declined over time, while IgG avidity index remained constant after reaching a plateau. The decline of antibody neutralization activity can be attributed to the reduction in antibody quantity rather than the deterioration of antibody quality, as measured by antibody avidity.

Rapid diagnosis of hantavirus disease with an IgG-avidity assay

The Lancet ◽  
1991 ◽  
Vol 338 (8779) ◽  
pp. 1353-1356 ◽  
Author(s):  
K. Hedman ◽  
A. Vaheri ◽  
M. Brummer-Korvenkontio
Keyword(s):  
Rapid Diagnosis ◽  
Avidity Assay ◽  
Igg Avidity

scholarly journals Use of an Anti-Hepatitis C Virus (HCV) IgG Avidity Assay To Identify Recent HCV Infection

2010 ◽  
Vol 48 (9) ◽  
pp. 3281-3287 ◽  
Author(s):  
C. Gaudy-Graffin ◽  
G. Lesage ◽  
I. Kousignian ◽  
S. Laperche ◽  
A. Girault ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Hcv Infection ◽  
Avidity Assay ◽  
Igg Avidity

scholarly journals Clinical evaluation of the Roche Elecsys® CMV IgG Avidity assay

2014 ◽  
Vol 33 (8) ◽  
pp. 1365-1369 ◽  
Author(s):  
C. Vauloup-Fellous ◽  
T. Lazzarotto ◽  
M. G. Revello ◽  
L. Grangeot-Keros
Keyword(s):  
Clinical Evaluation ◽  
Avidity Assay ◽  
Igg Avidity

scholarly journals Evaluation of the New Elecsys Toxo IgG Avidity Assay for Toxoplasmosis and New Insights into the Interpretation of Avidity Results

2012 ◽  
Vol 19 (11) ◽  
pp. 1838-1843 ◽  
Author(s):  
Jean-Benjamin Murat ◽  
Coralie L'Ollivier ◽  
Hélène Fricker Hidalgo ◽  
Jacqueline Franck ◽  
Hervé Pelloux ◽  
...  
Keyword(s):  
Severe Disease ◽  
High Avidity ◽  
Serum Samples ◽  
Recent Infection ◽  
Content Type ◽  
Additional Information ◽  
Avidity Assay ◽  
Igg Avidity ◽  
Acute Toxoplasmosis ◽  
Almost All

ABSTRACTDetection and treatment of acute toxoplasmosis during pregnancy can avoid severe disease of the fetus. In this context, assessment of anti-ToxoplasmaIgG avidity has been shown to exclude recent infection. The Elecsys Toxo IgG and IgM assays (Roche Diagnostics) have been validated for screening pregnant women and a new assay, Elecsys Toxo IgG Avidity, was recently developed. Our aims were to investigate the performance characteristics of this new avidity assay and explore whether additional information can be provided by avidity assays. The Elecsys assay was compared with the Vidas (bioMérieux) and Architect (Abbott) Avidity assays using two sets of serum samples (n= 291 andn= 255). The rate of general agreement between the Elecsys and Vidas assays was 74%, and that between the Elecsys and Architect assays was 83%. For 11% of the serum samples, avidity was high with the Vidas assay and within the gray zone with the Elecsys assay. None of the assays detected high-avidity antibodies in serum taken <4 months after infection. Avidity values of >90% were exclusively reported in sera taken >9 months after infection by the Elecsys and Architect assays. Almost all avidities of <19% with the Elecsys assay and <17% with the Architect assay corresponded to sera taken <3 and <2 months after infection, respectively. The Elecsys IgG Avidity assay can be used to exclude recent infection. New ways of interpreting the avidity result are also suggested: very high or low values could exclude infections within the last 9 months or help to confirm a recent infection, respectively. However, these potential interpretations require further investigation.

IgG avidity assay firms up the diagnosis of acute toxoplasmosis on the first serum sample in immunocompetent pregnant women

2007 ◽  
Vol 58 (1) ◽  
pp. 83-88 ◽  
Author(s):  
Ermanno Candolfi ◽  
Rebecca Pastor ◽  
Rachel Huber ◽  
Denis Filisetti ◽  
Odile Villard
Keyword(s):  
Pregnant Women ◽  
Serum Sample ◽  
Avidity Assay ◽  
Igg Avidity ◽  
Acute Toxoplasmosis

scholarly journals Erratum: Temporal dynamics of Puumala hantavirus infection in cyclic populations of bank voles

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Liina Voutilainen ◽  
Eva R. Kallio ◽  
Jukka Niemimaa ◽  
Olli Vapalahti ◽  
Heikki Henttonen
Keyword(s):  
Temporal Dynamics ◽  
Hantavirus Infection ◽  
Bank Voles ◽  
Cyclic Populations
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