Two-dimensional flow cytometric analysis of intrahepatic lymphocyte subsets from patients with chronic hepatitis

Abstract Background The purpose of this study is to investigate the aqueous humor (AH) T lymphocyte subsets and cytokines of acute retinal necrosis (ARN) to elucidate the immunologic inflammatory features of this disorder. Methods Three patients with ARN infected with varicella zoster virus (VZV) who underwent multiple intravitreal injections of ganciclovir were enrolled in this study. The control group consisted of four non-infectious patients with acute anterior uveitis (AAU). Flow cytometric analysis was performed on the lymphocyte subsets from the AH and peripheral blood (PB) samples during the active phase of intraocular inflammation. Five inflammatory cytokines were measured in each AH sample and various clinical characteristics were also assessed. Results VZV deoxyribonucleic acid (DNA) was detected by real-time polymerase chain reaction (PCR) in AH from all the ARN patients, who showed higher CD8+ T lymphocytes population in AH than the AAU patients (p = 0.006). CD4/CD8 ratios of T lymphocytes and the percentage of CD8 + CD25+ T lymphocytes in AH were significantly lower in ARN than in AAU (p = 0.006; p = 0.012). In the ARN patients, the percentages of CD4+ and CD8+ T lymphocytes in AH were higher than those found in PB. The percentage of CD4 + CD25+ T lymphocytes in AH was significantly higher than the proportion in PB in the AAU patients (p = 0.001). Immunoregulatory cytokine Interleukin-10 in AH was significantly elevated in the ARN patients in comparison with the case of the AAU patients (p = 0.036). In ARN, the copy number of VZV DNA in AH positively correlated with the percentage of CD8+ T lymphocytes in AH and negatively correlated with the CD4/CD8 ratio in AH during the course of disease treatment (p = 0.009, r = 0.92; p = 0.039, r = − 0.834). Conclusion The ARN patients caused by VZV had different intraocular T lymphocyte subsets and cytokines profile than those of the non-infectious patients. High percentages of CD8+ T lymphocytes and low CD4/CD8 T cell ratios may be a potential biomarker for diagnosis of viral-infectious uveitis. T lymphocytes examination at the inflammatory sites has the potential to become a useful research tool for differentiating viral and non-viral uveitis.

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Two-dimensional flow field distribution characteristics of flocking drainage pipes in tunnel

Open Physics ◽

10.1515/phys-2020-0013 ◽

2020 ◽

Vol 18 (1) ◽

pp. 139-148

Author(s):

Shiyang Liu ◽

Xuefu Zhang ◽

Feng Gao ◽

Liangwen Wei ◽

Qiang Liu ◽

...

Keyword(s):

Flow Field ◽

Field Distribution ◽

Rapid Development ◽

Maximum Velocity ◽

Distribution Characteristics ◽

Two Dimensional ◽

Dimensional Flow ◽

Laboratory Test Results ◽

Transverse Distance ◽

Two Dimensional Flow

AbstractWith the rapid development of traffic infrastructure in China, the problem of crystal plugging of tunnel drainage pipes becomes increasingly salient. In order to build a mechanism that is resilient to the crystal plugging of flocking drainage pipes, the present study used the numerical simulation to analyze the two-dimensional flow field distribution characteristics of flocking drainage pipes under different flocking spacings. Then, the results were compared with the laboratory test results. According to the results, the maximum velocity distribution in the flow field of flocking drainage pipes is closely related to the transverse distance h of the fluff, while the longitudinal distance h of the fluff causes little effect; when the transverse distance h of the fluff is less than 6.25D (D refers to the diameter of the fluff), the velocity between the adjacent transverse fluffs will be increased by more than 10%. Moreover, the velocity of the upstream and downstream fluffs will be decreased by 90% compared with that of the inlet; the crystal distribution can be more obvious in the place with larger velocity while it is less at the lower flow rate. The results can provide theoretical support for building a mechanism to deal with and remove the crystallization of flocking drainage pipes.

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