General expansions for nuclear surface and curvature energy coefficients in series of volume terms and surface moments

Cytochalasin B, a mould metabolite from Helminthosporium dermatioideum has been shown to interfere with specific cell activities such as cytoplasmic cleavage and cell movement. Cells undergoing nuclear division in the presence of cytochalasin B are unable to complete the separation of the resulting daughter cells. In time-lapse studies, the daughter cells coalesce after an initial unsuccessful attempt at separation and form large multinucleate polyploid cells. The present report describes the fine structure of the large polyploid cells induced in Earle's L-cell monolayer cultures by exposure to cytochalasin B (lγ/ml) for 92 hours.In the present material we have seen as many as 7 nuclei in these polyploid cells. Treatment with cytochalasin B for longer periods of time (6 to 7 days, with one medium change on the 3rd day) did not increase the number of nuclei beyond the 7 nuclei stage. Figure 1 shows a large polyploid cell with four nuclei. These nuclei are indistinguishable in their fine structure from those of the cells from control cultures but often show unusually large numbers of cytoplasmic invaginations and extensions of the nuclear surface (Figure 2).

Download Full-text

Detection and mapping of interactions between chromatin and the nuclear envelope in drosophila embryos

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100140191 ◽

1995 ◽

Vol 53 ◽

pp. 764-765

Author(s):

W.F. Marshall ◽

A.F. Dernburg ◽

B. Harmon ◽

J.W. Sedat

Keyword(s):

Nuclear Envelope ◽

Chromatin Condensation ◽

Three Dimensional ◽

Physiological Role ◽

Nuclear Surface ◽

Intact Cells ◽

Molecular Determinants ◽

Surface Harmonic ◽

Drosophila Embryos

Interactions between chromatin and nuclear envelope (NE) have been implicated in chromatin condensation, gene regulation, nuclear reassembly, and organization of chromosomes within the nucleus. To further investigate the physiological role played by such interactions, it will be necessary to determine which loci specifically interact with the nuclear envelope. This will not only facilitate identification of the molecular determinants of this interaction, but will also allow manipulation of the pattern of chromatin-NE interactions to probe possible functions. We have developed a microscopic approach to detect and map chromatin-NE interactions inside intact cells.Fluorescence in situ hybridization (FISH) is used to localize specific chromosomal regions within the nucleus of Drosophila embryos and anti-lamin immunofluorescence is used to detect the nuclear envelope. Widefield deconvolution microscopy is then used to obtain a three-dimensional image of the sample (Fig. 1). The nuclear surface is represented by a surface-harmonic expansion (Fig 2). A statistical test for association of the FISH spot with the surface is then performed.

Download Full-text

The Shikani HME: A New Tracheostomy Heat and Moisture Exchanger

Journal of Speech Language and Hearing Research ◽

10.1044/2020_jslhr-19-00107 ◽

2020 ◽

Vol 63 (9) ◽

pp. 2921-2929

Author(s):

Alan H. Shikani ◽

Elamin M. Elamin ◽

Andrew C. Miller

Keyword(s):

Ex Vivo ◽

Moisture Loss ◽

Speaking Valve ◽

Time Zero ◽

In Series ◽

Turbulent Airflow ◽

The Difference ◽

Loss Efficiency ◽

Heat And Moisture ◽

Lung Simulation

Purpose Tracheostomy patients face many adversities including loss of phonation and essential airway functions including air filtering, warming, and humidification. Heat and moisture exchangers (HMEs) facilitate humidification and filtering of inspired air. The Shikani HME (S-HME) is a novel turbulent airflow HME that may be used in-line with the Shikani Speaking Valve (SSV), allowing for uniquely preserved phonation during humidification. The aims of this study were to (a) compare the airflow resistance ( R airflow ) and humidification efficiency of the S-HME and the Mallinckrodt Tracheolife II tracheostomy HME (M-HME) when dry (time zero) and wet (after 24 hr) and (b) determine if in-line application of the S-HME with a tracheostomy speaking valve significantly increases R airflow over a tracheostomy speaking valve alone (whether SSV or Passy Muir Valve [PMV]). Method A prospective observational ex vivo study was conducted using a pneumotachometer lung simulation unit to measure airflow ( Q ) amplitude and R airflow , as indicated by a pressure drop ( P Drop ) across the device (S-HME, M-HME, SSV + S-HME, and PMV). Additionally, P Drop was studied for the S-HME and M-HME when dry at time zero (T 0 ) and after 24 hr of moisture testing (T 24 ) at Q of 0.5, 1, and 1.5 L/s. Results R airflow was significantly less for the S-HME than M-HME (T 0 and T 24 ). R airflow of the SSV + S-HME in series did not significant increase R airflow over the SSV or PMV alone. Moisture loss efficiency trended toward greater efficiency for the S-HME; however, the difference was not statistically significant. Conclusions The turbulent flow S-HME provides heat and moisture exchange with similar or greater efficacy than the widely used laminar airflow M-HME, but with significantly lower resistance. The S-HME also allows the innovative advantage of in-line use with the SSV, hence allowing concurrent humidification and phonation during application, without having to manipulate either device.

Download Full-text

Corrosion(Fifth in Series)- Corrosion Testing: Aeration and Expressions for Corrosion Rate

Industrial & Engineering Chemistry ◽

10.1021/ie50537a600 ◽

1954 ◽

Vol 46 (9) ◽

pp. 85-88

Author(s):

Mars Fontana

Keyword(s):

Corrosion Rate ◽

Corrosion Testing ◽

In Series

Download Full-text

Status of nondestructive control of transport function metal products at JSC EVRAZ NTMK

Ferrous Metallurgy Bulletin of Scientific Technical and Economic Information ◽

10.32339/0135-5910-2020-6-586-590 ◽

2020 ◽

Vol 76 (6) ◽

pp. 586-590

Author(s):

S. P. Bersenev ◽

E. M. Slobtsova

Keyword(s):

Crack Detection ◽

Middle Part ◽

Control Line ◽

Magnetic Powder ◽

Nondestructive Control ◽

Automated Control ◽

Ultrasonic Control ◽

Acoustic Contact ◽

In Series

Achievements in the area of automated ultrasonic control of quality of rails, solid-rolled wheels and tyres, wheels magnetic powder crack detection, carried out at JSC EVRAZ NTMK. The 100% nondestructive control is accomplished by automated control in series at two ultrasonic facilities RWI-01 and four facilities УМКК-1 of magnetic powder control, installed into the exit control line in the wheel-tyre shop. Diagram of location, converters displacement and control operations in the process of control at the facility RWI-01 presented, as well as the structural diagram of the facility УМКК-1. The automated ultrasonic control of rough tyres is made in the tyres control line of the wheel-tyre shop at the facility УКБ-1Д. The facility enables to control internal defects of tyres in radial, axis and circular directions of radiation. Possibilities of the facility УКБ-1Д software were shown. Nondestructive control of railway rails is made at two facilities, comprising the automated control line of the rail and structural shop. The УКР-64Э facility of automated ultrasonic rails control is intended to reveal defects in the area of head, web and middle part of rail foot by pulse echo-method with a immersion acoustic contact. The diagram of rail P65 at the facility УКР-64Э control presented. To reveal defects of the macrostructure in the area of rail head and web by mirror-shadow method, an ultrasonic noncontact electromagnetic-acoustic facility is used. It was noted, that implementation of the 100% nondestructive control into the technology of rolled stuff production enabled to increase the quality of products supplied to customers and to increase their competiveness.

Download Full-text

Dimensions of plane and solid angles and their units in the International System of Units (SI)

Izmeritel`naya Tekhnika ◽

10.32446/0368-1025it.2020-10-26-32 ◽

2020 ◽

pp. 26-32

Author(s):

M. I. Kalinin ◽

L. K. Isaev ◽

F. V. Bulygin

Keyword(s):

Solid Angle ◽

International System ◽

International Committee ◽

Plane Angle ◽

Arc Length ◽

Weights And Measures ◽

International System Of Units ◽

System Of Units ◽

In Series ◽

Solid Angles

The situation that has developed in the International System of Units (SI) as a result of adopting the recommendation of the International Committee of Weights and Measures (CIPM) in 1980, which proposed to consider plane and solid angles as dimensionless derived quantities, is analyzed. It is shown that the basis for such a solution was a misunderstanding of the mathematical formula relating the arc length of a circle with its radius and corresponding central angle, as well as of the expansions of trigonometric functions in series. From the analysis presented in the article, it follows that a plane angle does not depend on any of the SI quantities and should be assigned to the base quantities, and its unit, the radian, should be added to the base SI units. A solid angle, in this case, turns out to be a derived quantity of a plane angle. Its unit, the steradian, is a coherent derived unit equal to the square radian.

Download Full-text