Reactivation of a glycerinated model of Amoeba

PROTOPLASMA ◽  
1981 ◽  
Vol 109 (1-2) ◽  
pp. 127-142 ◽  
Author(s):  
K. Kuroda ◽  
S. Sonobe
Keyword(s):  
Glycerinated Model

Kinetic Studies of the Flagellar Movement of Sea-Urchin Spermatozoa

1969 ◽  
Vol 50 (1) ◽  
pp. 203-222
Author(s):  
M. E. J. HOLWILL
Keyword(s):  
Dielectric Constant ◽  
Ionic Strength ◽  
Sea Urchin ◽  
Activation Parameters ◽  
Kinetic Studies ◽  
Basic Part ◽  
Substrate Complex ◽  
Enzyme Substrate ◽  
Glycerinated Model ◽  
Rate Limiting

1. The movements of intact and glycerinated spermatozoa from two species of sea-urchin were examined in the range of temperature between 5 and 30° C. The variations of the frequency of the glycerinated spermatozoa with changing pH, dielectric constant and ionic strength were examined. 2. From the temperature studies values for activation entropies and enthalpies were obtained that pertain to the chemical reaction which limits the frequency. 3. Within the limits of experimental error the activation parameters are identical for the intact spermatozoa and for the glycerinated model of the same species. The results are consistent with the hypothesis that the rate-limiting reaction is the breakdown of an enzyme-substrate complex. 4. The pH studies suggest that neither the acidic nor the basic part of the enzyme is involved in complex formation but that both participate in the breakdown of the complex. 5. The results obtained from the studies of dielectric constant are interpreted in terms of a model for the breakdown of the enzyme-substrate complex involving the separation of several spherical charges. 6. The studies of pH and ionic strength suggest that both 14 S and 30 S dynein participate in the mechano-chemical process responsible for bending the flagellum.

Ultrastructural aspects of a glycerinated model ofAmoeba proteus

PROTOPLASMA ◽  
1986 ◽  
Vol 130 (1) ◽  
pp. 41-50 ◽  
Author(s):  
S. Sonobe ◽  
K. Kuroda
Keyword(s):  
Glycerinated Model
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