Permeabilization ofNitella internodal cell with electrical pulses

PROTOPLASMA ◽  
1983 ◽  
Vol 117 (1) ◽  
pp. 93-96 ◽  
Author(s):  
T. Shimmen ◽  
M. Tazawa
Keyword(s):  
Internodal Cell ◽  
Electrical Pulses

Photoconductive gating of picosecond electrical pulses on thinned-silicon substrates

2000 ◽  
Vol 36 (14) ◽  
pp. 1225 ◽  
Author(s):  
J.F. Holzman ◽  
F.E. Vermeulen ◽  
B.W. Arnold ◽  
A.Y. Elezzabi
Keyword(s):  
Silicon Substrates ◽  
Electrical Pulses

scholarly journals Real-Time High-Level Acute Pain Detection Using a Smartphone and a Wrist-Worn Electrodermal Activity Sensor

Sensors ◽  
2021 ◽  
Vol 21 (12) ◽  
pp. 3956
Author(s):  
Youngsun Kong ◽  
Hugo F. Posada-Quintero ◽  
Ki H. Chon
Keyword(s):  
Real Time ◽  
Electrodermal Activity ◽  
Smartphone Application ◽  
Real Time Processing ◽  
Pain Stimulation ◽  
Significant Difference ◽  
Before And After ◽  
Electrical Pulses ◽  
Pain Detection ◽  
High Level

The subjectiveness of pain can lead to inaccurate prescribing of pain medication, which can exacerbate drug addiction and overdose. Given that pain is often experienced in patients’ homes, there is an urgent need for ambulatory devices that can quantify pain in real-time. We implemented three time- and frequency-domain electrodermal activity (EDA) indices in our smartphone application that collects EDA signals using a wrist-worn device. We then evaluated our computational algorithms using thermal grill data from ten subjects. The thermal grill delivered a level of pain that was calibrated for each subject to be 8 out of 10 on a visual analog scale (VAS). Furthermore, we simulated the real-time processing of the smartphone application using a dataset pre-collected from another group of fifteen subjects who underwent pain stimulation using electrical pulses, which elicited a VAS pain score level 7 out of 10. All EDA features showed significant difference between painless and pain segments, termed for the 5-s segments before and after each pain stimulus. Random forest showed the highest accuracy in detecting pain, 81.5%, with 78.9% sensitivity and 84.2% specificity with leave-one-subject-out cross-validation approach. Our results show the potential of a smartphone application to provide near real-time objective pain detection.

scholarly journals Turnover of protein-bound serine phosphate in respiring slices of guinea-pig cerebral cortex. Effects of putative transmitters, tetrodotoxin and other agents

1973 ◽  
Vol 132 (3) ◽  
pp. 475-482 ◽  
Author(s):  
Martin Reddington ◽  
Richard Rodnight ◽  
Michael Williams
Keyword(s):  
Cerebral Cortex ◽  
Cyclic Amp ◽  
Guinea Pig ◽  
Electrical Pulses ◽  
Phosphorus Turnover ◽  
Serine Phosphate ◽  
Stimulation Of

1. The effect of various agents on the turnover of protein-bound phosphorus in respiring slices of cerebral cortex was studied. 2. Confirming previous work turnover was increased by the application of electrical pulses for 10s to the tissue. 3. Turnover was also increased by exposure of the slices for 10min to noradrenaline (0.5mm), 5-hydroxytryptamine (1μm) and histamine (0.1mm). 4. When slices were stimulated by electrical pulses in the presence of histamine the increase in turnover was the sum of the responses given by each agent above, suggesting that different phosphorylating systems were involved. 5. Tetrodotoxin (0.5μm) blocked the increased turnover due to electrical pulses, but not that due to histamine. Tetrodotoxin also prevented the increase in tissue cyclic AMP content caused by the application of electrical pulses. 6. Phosphoprotein turnover was not affected by adenosine, despite the increase in tissue cyclic AMP content given by this agent. 7. Adenosine blocked the phosphoprotein response to histamine, but did not affect the response to electrical pulses. 8. The results are discussed in relation to the hypothesis that the stimulation of protein phosphorus turnover by electrical pulses is secondary to the release of cyclic AMP in the tissue.

Introduction of exogenous DNA into Chlamydomonas reinhardtii by electroporation

1991 ◽  
Vol 11 (4) ◽  
pp. 2328-2332
Author(s):  
L E Brown ◽  
S L Sprecher ◽  
L R Keller
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Plasmid Dna ◽  
Cell Walls ◽  
Intact Cell ◽  
Exogenous Dna ◽  
Electrical Pulses ◽  
Exogenous Plasmid

The fate of exogenous DNA introduced into Chlamydomonas reinhardtii by electroporation was analyzed. With single and double electrical pulses, plasmids as large as 14 kb were introduced into cells with and without intact cell walls. Within hours after introduction, exogenous plasmid DNA was associated with nuclei isolated from cells; several weeks after introduction, exogenous DNA was stably integrated into the Chlamydomonas genome. These studies establish electroporation as a method for introducing DNA, and potentially other molecules, into C. reinhardtii.

Repetitive Potentials Following Brief Electric Stimuli in a Hydroid

1961 ◽  
Vol 38 (3) ◽  
pp. 579-593
Author(s):  
ROBERT K. JOSEPHSON
Keyword(s):  
Electrical Stimulation ◽  
Average Velocity ◽  
Nervous Activity ◽  
Repetitive Stimulation ◽  
Conducting System ◽  
Threshold Intensity ◽  
Electric Shocks ◽  
Electrical Pulses ◽  
Minimum Interval ◽  
Shape And Size

1. Electrical pulses (amplitude -0.05 to -15 mV.; duration 20-120 msec.) have been recorded from the stolon of Cordylophora lacustris following stimulation. These pulses are propagated with an average velocity of 2.7 cm./sec. at 22° C. 2. Brief electric shocks of little more than threshold intensity can evoke bursts of pulses. The number of pulses in a burst increases with stimulus intensity, but the shape and size of individual pulses do not. 3. Repetitive stimulation causes facilitation of both size of single pulses and number of pulses in a burst. Refractory period, if present, is variable. The minimum interval between two pulses is about 200 msec. 4. Mechanical stimulation evokes pulses identical to those evoked by electrical stimulation. 5. The greater the number of pulses recorded in the stolon near a polyp, the greater and faster is the contraction of that polyp. 6. The number of pulses, but not their individual sizes, decreases with increasing distance from the point of stimulation. 7. It is concluded that conduction in the stolon and the electrical pulses are due to nervous activity and that the conducting system is a network having interneural junctions which sometimes require to be facilitated.

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