Somatic embryogenesis in suspension and suspension-derived callus cultures ofDactylis glomerata

PROTOPLASMA ◽  
1984 ◽  
Vol 122 (3) ◽  
pp. 196-202 ◽  
Author(s):  
D. J. Gray ◽  
B. V. Conger ◽  
G. E. Hanning
Keyword(s):  
Somatic Embryogenesis ◽  
Callus Cultures

scholarly journals Bioproduction of Diosgenin in Callus Cultures of Balanites aegyptiaca: Effect of Growth Regulators, Explants and Somatic Embryogenesis

2006 ◽  
Vol 1 (3) ◽  
pp. 1934578X0600100
Author(s):  
Bishnu P. Chapagain ◽  
Vinod Saharan ◽  
Dan Pelah ◽  
Ram C. Yadav ◽  
Zeev Wiesman
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Callus Formation ◽  
Basal Medium ◽  
Callus Cultures ◽  
Suspension Cultures ◽  
Balanites Aegyptiaca ◽  
Basal Media

This study describes the effects of plant growth regulators, explants, and somatic embryogenesis on in vitro production of the steroidal sapogenin, diosgenin, in callus cultures of the Balanites aegyptiaca (L.) Del.(desert date). Root, shoot, hypocotyl, and epicotyl callus culture of B. aegyptiaca, were raised on MS basal media supplemented with various combinations of either 2,4-D and NAA alone, or with BAP. The diosgenin content (on a dry weight basis) was found to be highest when calli were cultured in MS basal medium supplemented with 1.0 mg l−1 2,4-D alone and/or in combination with 0.5 mg l−1 BAP. However, the callus growth was highest in media supplemented with 2.5 or 3.0 mg l−1 2,4-D. MS basal media supplemented with 2,4-D 2.5 mg l−1 alone and in combination with 0.5 mg l−1 BAP induced pre-embryogenic callus formation on root cultures. When these pre-embryogenic callus cultures were used to establish cell suspension cultures, two growth densities were obtained in embryogenic suspension cultures, inducing clusters of somatic embryos at various stages of development. The maximum number of somatic embryos were obtained at the fifth week on the medium supplemented with 1.0 mg l−1 2,4-D. However, the diosgenin content in these somatic cells was found to be lower compared to the explant calluses. This study revealed that production of diosgenin in callus cultures of B. aegyptiaca is possible, but the amount is significantly affected by the growth regulators, type of explants, and somatic embryogenesis.

Somatic embryogenesis from leaf and petiole callus cultures of Gossypium hirsutum L.

1986 ◽  
Vol 5 (6) ◽  
pp. 457-459 ◽  
Author(s):  
Nick J. Gawel ◽  
Arelli P. Rao ◽  
Carol D. Robacker
Keyword(s):  
Somatic Embryogenesis ◽  
Gossypium Hirsutum ◽  
Callus Cultures ◽  
Gossypium Hirsutum L

Somatic embryogenesis and plant regeneration from callus cultures of several species in the genus Tricyrtis

2004 ◽  
Vol 40 (3) ◽  
pp. 274-278 ◽  
Author(s):  
Masaru Nakano ◽  
Keiko Mizunashi ◽  
Shigefumi Tanaka ◽  
Toshinari Godo ◽  
Masashi Nakata ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Callus Cultures

The role of chitinases and glucanases in somatic embryogenesis of black pine and hybrid firs

2013 ◽  
Vol 8 (12) ◽  
pp. 1172-1182 ◽  
Author(s):  
Lenka Fráterová ◽  
Terézia Salaj ◽  
Ildikó Matušíková ◽  
Ján Salaj
Keyword(s):  
Somatic Embryogenesis ◽  
Culture Media ◽  
Extracellular Fluid ◽  
Abies Alba ◽  
Pinus Nigra ◽  
Callus Cultures ◽  
Growth Media ◽  
Large Variability ◽  
Embryogenic Cell ◽  
Embryogenic Potential

AbstractGlucanase and chitinase enzymes play an important role in different plant processes including defense against pathogens and morphogenesis. Moreover, their role in the processes of somatic embryogenesis has been demonstrated. It has been suggested, that the presence of this type of proteins might be a marker for embryogenic potential of callus cultures. In this work we screened for the presence of glucanases and chitinases in liquid growth media of a set of conifer embryogenic cell lines in order to find correlation with their embryogenic potential. We have found that none of the 12 chitinase isoforms detected in culture media of Pinus nigra Arn. or the nine chitinases detected in media with Abies alba × A. cephalonica and Abies alba × A. numidica embryogenic tissues could be linked to their embryogenic capacity. Similarly, none of the six glucanase isoforms detected in the extracellular fluid of Pinus nigra Arn. cultures can be assigned as a marker of embryogenic potential. Thus, our data indicate the large variability and doubtless importance of glucanases and chitinases for cell growth and development of somatic embryos, however, do not support the premise that they are markers of embryogenesis.

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