Cloning and characterization of endosymbiotic algae isolated from Paramecium bursaria

PROTOPLASMA ◽  
1998 ◽  
Vol 203 (1-2) ◽  
pp. 91-99 ◽  
Author(s):  
N. Nishihara ◽  
S. Horiike ◽  
T. Takahashi ◽  
T. Kosaka ◽  
Y. Shigenaka ◽  
...  
Keyword(s):  
Paramecium Bursaria ◽  
Endosymbiotic Algae

scholarly journals Characterization of a Novelcis-synandtrans-syn-IIPyrimidine Dimer Glycosylase/AP Lyase from a Eukaryotic Algal Virus,Paramecium bursaria chlorellaVirus-1

1998 ◽  
Vol 273 (21) ◽  
pp. 13136-13142 ◽  
Author(s):  
Amanda K. McCullough ◽  
Matthew T. Romberg ◽  
Simon Nyaga ◽  
Yuanfen Wei ◽  
Thomas G. Wood ◽  
...  
Keyword(s):  
Paramecium Bursaria ◽  
Algal Virus ◽  
Ap Lyase

Morphological features and isoenzyme characterization of endosymbiotic algae from green hydra

2009 ◽  
Vol 284 (1-2) ◽  
pp. 33-39 ◽  
Author(s):  
Goran Kovačević ◽  
Sandra Radić ◽  
Biserka Jelenčić ◽  
Mirjana Kalafatić ◽  
Hrvoje Posilović ◽  
...  
Keyword(s):  
Morphological Features ◽  
Green Hydra ◽  
Endosymbiotic Algae

The role of endosymbiotic algae in photoaccumulation of green Paramecium bursaria

Planta ◽  
1981 ◽  
Vol 152 (3) ◽  
pp. 268-271 ◽  
Author(s):  
D�rte Niess ◽  
Werner Reisser ◽  
Wolfgang Wiessner
Keyword(s):  
Paramecium Bursaria ◽  
Endosymbiotic Algae

Photosynthetic efficiency of endosymbiotic algae of Paramecium bursaria originating from locations with cold and warm climates

2018 ◽  
Vol 47 (2) ◽  
pp. 202-210
Author(s):  
Katarzyna Możdżeń ◽  
Patrycja Z. Leśnicka ◽  
Tomasz Burnecki ◽  
Sylwia Śliwińska-Wilczewska ◽  
Andrzej Skoczowski ◽  
...  
Keyword(s):  
Photosynthetic Activity ◽  
Cold Climate ◽  
Paramecium Bursaria ◽  
Control Group ◽  
Constant Darkness ◽  
Light Conditions ◽  
Warm Climate ◽  
Symbiotic Algae ◽  
Different Temperatures ◽  
Endosymbiotic Algae

Abstract Paramecium bursaria (Ciliophora) is a cosmopolitan unicellular organism that plays a significant role in aquatic ecosystems. P. bursaria contains symbiotic algae and this association is a mutual symbiosis. The aim of the present study was to determine the activity of photosystem II (PSII) in Chlorella sp. inside P. bursaria cells. Ciliates were incubated for 7 days at different temperatures from 6 to 18°C, under the circadian cycle: 12 h light/12 h dark, at light intensity of 200 μmol m-2 s-1 and under constant darkness conditions. The control group was kept at a temperature of 18°C under constant light conditions. Changes in PSII were monitored using different fluorescence parameters. Differences in responses between endosymbiotic algae of two P. bursaria strains – Ard7 from a warm climate and KD64 from a cold climate – were determined. The highest photosynthetic activity of P. bursaria green endosymbionts was observed at a temperature of 18°C, regardless of the light conditions. Algae from warm climate were more sensitive to cold temperature stress than algae from P. bursaria collected in cold climate.

scholarly journals Characterization of the RNA-interference pathway as a tool for reverse genetic analysis in the nascent phototrophic endosymbiosis, Paramecium bursaria

2021 ◽  
Vol 8 (4) ◽  
Author(s):  
Benjamin H. Jenkins ◽  
Finlay Maguire ◽  
Guy Leonard ◽  
Joshua D. Eaton ◽  
Steven West ◽  
...  
Keyword(s):  
Rna Interference ◽  
Paramecium Bursaria ◽  
Rnai Pathway ◽  
Reverse Genetic ◽  
Knock Down ◽  
Protein Encoding ◽  
Host Growth ◽  
Rna Pathways ◽  
Si Rna

Endosymbiosis was fundamental for the evolution of eukaryotic complexity. Endosymbiotic interactions can be dissected through forward- and reverse-genetic experiments, such as RNA-interference (RNAi). However, distinguishing small (s)RNA pathways in a eukaryote–eukaryote endosymbiotic interaction is challenging. Here, we investigate the repertoire of RNAi pathway protein-encoding genes in the model nascent endosymbiotic system, Paramecium bursaria–Chlorella spp. Using comparative genomics and transcriptomics supported by phylogenetics, we identify essential proteome components of the small interfering (si)RNA, scan (scn)RNA and internal eliminated sequence (ies)RNA pathways. Our analyses reveal that copies of these components have been retained throughout successive whole genome duplication (WGD) events in the Paramecium clade. We validate feeding-induced siRNA-based RNAi in P. bursaria via knock-down of the splicing factor, u2af1 , which we show to be crucial to host growth. Finally, using simultaneous knock-down ‘paradox’ controls to rescue the effect of u2af1 knock-down, we demonstrate that feeding-induced RNAi in P. bursaria is dependent upon a core pathway of host-encoded Dcr1 , Piwi and Pds1 components. Our experiments confirm the presence of a functional, host-derived RNAi pathway in P. bursaria that generates 23-nt siRNA, validating the use of the P. bursaria – Chlorella spp. system to investigate the genetic basis of a nascent endosymbiosis.

scholarly journals The photosynthetic activity of Paramecium bursaria endosymbiotic algae in varying temperature conditions

2017 ◽  
Author(s):  
Katarzyna Możdżeń ◽  
Patrycja Zagata-Leśnicka ◽  
Mateusz Ślęczka ◽  
Magdalena Greczek-Stachura
Keyword(s):  
Fluorescence Spectra ◽  
Photosynthetic Activity ◽  
Red Light ◽  
Paramecium Bursaria ◽  
Green Fluorescence ◽  
Chlorella Sp ◽  
Fluorescence Measurements ◽  
Higher Temperature ◽  
Endosymbiotic Algae ◽  
C 30

The aim of this study was to investigate the effect of higher temperatures on the photosynthesis of endosymbiotic Chlorella sp. of two Paramecium bursaria Ehrenberg 1831 strains originating from regions with a warmer and colder climate (Ardmore – USA and Kamchatka – Russia, respectively). After seven days of protozoa incubation at 18°C (control), 21°C, 24°C, 27°C, 30°C and 33°C, the chlorophyll a fluorescence measurements were carried out and fluorescence spectra were measured in blue-green and red light. As a result of the studies, a significant effect of higher temperature on the photosynthesis process of P. bursaria endosymbionts was observed. Weekly incubation at 33°C was lethal for both protozoan strains in comparison to the control temperature (18°C). The blue-green fluorescence spectra were characterised by marked peaks at 450 nm and 490 nm. Within the red light range, the peak was observed at about 690 nm with a lesser arm at 730 nm. Endosymbionts from Kamchatka were more sensitive to the temperature increase than algae from areas with relatively warm climates.

scholarly journals Isolation and characterization of a virus (CvV-BW1) that infects symbiotic algae of Paramecium bursaria in Lake Biwa, Japan

2010 ◽  
Vol 7 (1) ◽  
pp. 222 ◽  
Author(s):  
Ryo Hoshina ◽  
Mayumi Shimizu ◽  
Yoichi Makino ◽  
Yoshihiro Haruyama ◽  
Shin-ichiro Ueda ◽  
...  
Keyword(s):  
Lake Biwa ◽  
Paramecium Bursaria ◽  
Symbiotic Algae ◽  
Isolation And Characterization

scholarly journals Catalysis of Chlorovirus Production by the Foraging of Bursaria truncatella on Paramecia bursaria Containing Endosymbiotic Algae

2021 ◽  
Vol 9 (10) ◽  
pp. 2170
Author(s):  
Zeina T. Al-Ameeli ◽  
Maitham M. Al-Sammak ◽  
John P. DeLong ◽  
David D. Dunigan ◽  
James L. Van Van Etten
Keyword(s):  
Green Algae ◽  
Virus Replication ◽  
Paramecium Bursaria ◽  
Fecal Pellets ◽  
Population Sizes ◽  
Endosymbiotic Algae ◽  
Additional Support

Chloroviruses are large viruses that replicate in chlorella-like green algae and normally exist as mutualistic endosymbionts (referred to as zoochlorellae) in protists such as Paramecium bursaria. Chlorovirus populations rise and fall in indigenous waters through time; however, the factors involved in these virus fluctuations are still under investigation. Chloroviruses attach to the surface of P. bursaria but cannot infect their zoochlorellae hosts because the viruses cannot reach the zoochlorellae as long as they are in the symbiotic phase. Predators of P. bursaria, such as copepods and didinia, can bring chloroviruses into contact with zoochlorellae by disrupting the paramecia, which results in an increase in virus titers in microcosm experiments. Here, we report that another predator of P. bursaria, Bursaria truncatella, can also increase chlorovirus titers. After two days of foraging on P. bursaria, B. truncatella increased infectious chlorovirus abundance about 20 times above the controls. Shorter term foraging (3 h) resulted in a small increase of chlorovirus titers over the controls and more foraging generated more chloroviruses. Considering that B. truncatella does not release viable zoochlorellae either during foraging or through fecal pellets, where zoochlorellae could be infected by chlorovirus, we suggest a third pathway of predator virus catalysis. By engulfing the entire protist and digesting it slowly, virus replication can occur within the predator and some of the virus is passed out through a waste vacuole. These results provide additional support for the hypothesis that predators of P. bursaria are important drivers of chlorovirus population sizes and dynamics.

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