Light and electron microscopic cytochemistry of the epidermal mucoid cells ofAeolidia papillosa andCoryphella rufibranchialis (Mollusca, Nudibranchia)

PROTOPLASMA ◽  
1983 ◽  
Vol 114-114 (1-2) ◽  
pp. 14-22 ◽  
Author(s):  
K. J. Porter ◽  
E. R. Rivera
Keyword(s):  
Electron Microscopic ◽  
Electron Microscopic Cytochemistry ◽  
Mucoid Cells

EM of human atherosclerosis: Aortic fatty streaks with transitional lesion features

1992 ◽  
Vol 50 (1) ◽  
pp. 622-623
Author(s):  
K. Florian Klemp ◽  
J.R. Guyton
Keyword(s):  
Foam Cell ◽  
Cell Formation ◽  
Processing Technique ◽  
Foam Cell Formation ◽  
Tissue Preparation ◽  
Electron Microscopic ◽  
Extracellular Lipid ◽  
Electron Microscopic Cytochemistry ◽  
Human Atherosclerosis ◽  
Clinically Significant

The earliest distinctive lesions in human atherosclerosis are fatty streaks (FS), characterized initially by lipid-laden foam cell formation. Fibrous plaques (FP), the clinically significant lesions, differ from FS in several respects. In addition to foam cells, the FP also exhibit fibromuscular proliferation and a necrotic core region rich in extracellular lipid. The possible transition of FS into mature FP has long been debated, however. A subset of FS described by Katz etal., was intermediate in lipid composition between ordinary FS and FP. We investigated this hypothesis by electron microscopic cytochemistry by employing a tissue processing technique previously described by our laboratory. Osmium-tannic acid-paraphenylenediamine (OTAP) tissue preparation enabled ultrastructural analysis of lipid deposits to discern features characteristic of mature fibrous plaques.

Ultrastructural Cytochemical Study of Human Gastric Cancer: Observation of AKP ase,ACP ase,G6P ase,TPP ase and CO ase

1990 ◽  
Vol 48 (3) ◽  
pp. 254-255
Author(s):  
Dong Yuming ◽  
Yang Guanglin ◽  
Du Wei Dong ◽  
Xu Ai Liam
Keyword(s):  
Gastric Cancer ◽  
Gastric Epithelium ◽  
Human Gastric Cancer ◽  
Electron Microscopic ◽  
Cytochemical Study ◽  
Electron Microscopic Cytochemistry ◽  
Cytochemical Reaction ◽  
Set Up ◽  
Cancer Tissues ◽  
Cytochemical Technique

The activities and distributions of AKPase ,ACPase,G6Pase,TPPase and COase in human normal gastric mucosa and gastric cancer tissues were studied histochemically at light microscopic level. These enzymes are the marker enzymes of cell membrane lysosome endoplasmic reticulum, Golgi apparatus and mitochondrion objectively. On the basis of the research we set up a special ultrastructural cytochemical technique and first researched into gastric cancer domesticly. Ultrastructural cytochemistry is also called electron microscopic cytochemistry. This new technique possesses both the sensitivity of cytochemical reaction andi the high resolution of electron microscope. It is characterized by direct observation,exact localization and the combination morphology with function.The distributions of AKPase,ACPase,G6Pase,TPPase and COase in 14 cases of gastric cancer and 1 case of gastric Denign lesion were studied ultrastructurally. The results showed: 1. normal gastric epithelium had no AKPase reaction. The reaction of ACPase,G6Pase,TPPase and Coase were found in the corresponding organella, which were consistent with their function.

Electron microscopic evidence for the presence of an asialoglycoprotein receptor on isolated foetal rat hepatocyte surface

Development ◽  
1984 ◽  
Vol 79 (1) ◽  
pp. 41-51
Author(s):  
Laura Conti Devirgiliis ◽  
Luciana Dini ◽  
Salvatore Russo-Caia
Keyword(s):  
Binding Capacity ◽  
Asialoglycoprotein Receptor ◽  
Electron Microscopic ◽  
Adult Rat ◽  
Receptor Complexes ◽  
Developmental Age ◽  
Foetal Rat ◽  
Mammalian Liver ◽  
Electron Microscopic Cytochemistry ◽  
Entire Cell

The ontogeny of asialoglycoprotein receptor was investigated by electron microscopic cytochemistry in hepatocytes isolated from foetal and adult rat. The binding capacity for asialofetuin coupled to horseradish peroxidase was lacking before the 18th day of intrauterine life; it arises at this time and increases with developmental age. The ligand-receptor complexes form small patches. The distribution pattern of positivity is very similar in pre and postnatal age, covering the entire cell surface. These results indicate a rather late appearance of the galactose-binding capacity related to the asialoglycoprotein clearance function, which is typical of adult mammalian liver.

scholarly journals DNA dependence and inhibition by novobiocin and coumermycin of the nucleolar adenosine triphosphatase (ATPase) of human fibroblasts.

1982 ◽  
Vol 30 (4) ◽  
pp. 364-370 ◽  
Author(s):  
N Fox ◽  
G P Studzinski
Keyword(s):  
Adenosine Triphosphatase ◽  
Elevated Temperatures ◽  
Human Fibroblasts ◽  
Nucleolar Organizer ◽  
Electron Microscopic ◽  
Dna Topoisomerase ◽  
B Subunit ◽  
Electron Microscopic Cytochemistry ◽  
Nucleolar Chromatin ◽  
A Subunit

We have recently demonstrated by electron microscopic cytochemical methods that unfixed human fibroblasts exhibit intense MG2+ dependent adenosine triphosphatase (nATPase) activity in circumscribed areas of the cell nucleoli. The nATPase was specific for ATP and dATP and was inhibited by other ribonucleoside triphosphates. Its intranucleolar localization relative to nucleolar chromatin, and segregation into nucleolar zones after actinomycin treatment of the cells, suggested that the reaction took place in fibrillar centers. This ATPase has now been further characterized by electron microscopic cytochemistry. It was determined that short fixation permitted retention of most of the ATPase activity, and that the enzyme was active at high ionic strength (up to 400 mM KCl), but that the enzyme activity was very sensitive to elevated temperatures. DNA dependence of the enzyme was shown by inhibition of the reaction by DNase pretreatment in parallel with the removal of DNA from the cell, while pretreatment with RNase had no significant effect. The nATPase activity was also selectively inhibited by treatment of the cells with antagonists of the B subunit of DNA gyrase, novobiocin, and coumermycin, but not by nalidixic or oxolinic acids, which interfere with the A subunit of gyrase. Inhibitors of RNA synthesis, actinomycin D and aminonucleoside of puromycin, potentiate rather than inhibit nATPase reaction. The results suggest that nATPase functions to alter the degree of supercoiling or catenation of nucleolar organizer DNA, and is in reality a DNA topoisomerase that hydrolyzes ATP during its action.

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