Auxin-induced longitudinal-to-transverse reorientation of cortical microtubules in nonelongating epidermal cells of azuki bean epicotyls

From immunofluorescence microscopy it has been cortical microtubules form whole-cell arrays. This has clearly seen in cylindrical hairs where the existence testifies to the continuity of the array around the cell. It is not, however, clear how microtubules pack polyhedral cells with multiple, angled facets. In problem, elongated and isodiametric cells in the stramonium L. were subjected to anti-tubulin avoiding distortion by cellulase treatment and air- sections were then deblurred by computer, the digitized, reconstructed and then rotated in order to arrangement of microtubules along the anticlinal walls This established several things. Microtubules tend to any one cell face; they form transverse, oblique or except that some walls bear a crisscross arrangement. cells, microtubules clearly form helices. In the cells, transversely wound microtubules are confirmed continuous from one face to another and probably, constitute helices. Microtubules on oblique end walls continue onto the side walls and do not form a microtubules can be ordered upon two adjacent facets, with respect to the stem's axis need not necessarily both facets, i.e. overall alignment can change at the isodiametric epidermal cells, microtubules can one cell facet to another. However, where microtubules anticlinal walls spill over onto a periclinal wall at a crisscross arrangement is set up. This is attributed geometrical problem of fitting parallel lines around polyhedra. Despite crossing over one another, the walls are nevertheless continuous with MTs on the side conclusion, in elongated cells the arrays still various pitch: in isodiametric cells (where the walls non-orthogonal angles to one another) the integrity of appears to be preserved by microtubules crossing over what is termed a ‘sacrificial’ face. The overriding microtubules to form an integral array regardless of

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Hypergravity induces reorientation of cortical microtubules and modifies growth anisotropy in azuki bean epicotyls

Planta ◽

10.1007/s00425-006-0319-8 ◽

2006 ◽

Vol 224 (6) ◽

pp. 1485-1494 ◽

Cited By ~ 35

Author(s):

Kouichi Soga ◽

Kazuyuki Wakabayashi ◽

Seiichiro Kamisaka ◽

Takayuki Hoson

Keyword(s):

Azuki Bean ◽

Cortical Microtubules ◽

Growth Anisotropy

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The arrangement of microtubules in leaves of monocotyledonous and dicotyledonous plants

Canadian Journal of Botany ◽

10.1139/b89-430 ◽

1989 ◽

Vol 67 (12) ◽

pp. 3506-3512 ◽

Cited By ~ 10

Author(s):

Taizo Hogetsu

Keyword(s):

Cell Wall ◽

Zea Mays ◽

Avena Sativa ◽

Epidermal Cells ◽

Cellulose Microfibrils ◽

Cortical Microtubules ◽

Monocotyledonous Plant ◽

Pisum Sativum L ◽

Dicotyledonous Plants ◽

Parenchymal Cells

The first leaf of Avena sativa L., a monocotyledonous plant, grows in a region that lies within 10 mm of the base of the leaf. Cells in that region elongate longitudinally but hardly expand laterally. The orientation of cortical microtubules in the elongating region is transverse in both epidermal and parenchymal cells. The same features of the arrangement of microtubules are also observed in the leaves of Zea mays. Cellulose microfibrils in the cell wall are coaligned with microtubules, lying approximately transverse to the axis of elongation, as if they function as hoops to facilitate the longitudinal elongation of the cell. The cells of growing leaves of Pisum sativum L., a dicotyledonous plant, expand superficially in every direction at every point on the leaf. Cortical microtubules lining the outer walls of epidermal cells are arranged randomly or in parallel. The parallel microtubules are oriented in various directions. In the outer walls of epidermal cells of growing leaves, areas with different predominant orientations of microfibrils are found within a single cell, consistent with the arrangement of microtubules. These results indicate that the orientation of cortical microtubules is correlated with the orientation of microfibrils and the direction of growth in growing leaves of both monocotyledons and dicotyledons, suggesting the involvement of cortical microtubules in control of the direction of growth in leaves.

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