A novel tubular array associated with protein bodies in the rough endoplasmic reticulum ofopaque-2 maize

PROTOPLASMA ◽  
1996 ◽  
Vol 195 (1-4) ◽  
pp. 68-77 ◽  
Author(s):  
C. R. Lending
Keyword(s):  
Endoplasmic Reticulum ◽  
Rough Endoplasmic Reticulum ◽  
Protein Bodies ◽  
Tubular Array

Ébauches intracytoplasmiques de grains protéiques dans des cotylédons de Raphanus en développement

1986 ◽  
Vol 64 (12) ◽  
pp. 2887-2895
Author(s):  
Louis Genevès ◽  
Jacques Rutin
Keyword(s):  
Endoplasmic Reticulum ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Rough Endoplasmic Reticulum ◽  
Early Phase ◽  
Acid Phosphatase Activity ◽  
Early Stage ◽  
Protein Bodies ◽  
Meristematic Cells ◽  
Size And Structure

Protein bodies were characterized at an early stage of their maturation in thin green cotyledons of developing radish embryos. They appeared as granules in the cytoplasm of meristematic cells. Their diameter (0.5 to 1 μm) was in the range of that of mitochondria. They were distinguished from vacuoles by their morphology, size, and structure. Some appeared to be associated with cisternae of endoplasmic reticulum or dictyosomes (permanganic fixations). Their evolution was synchronous in the cell and also in the cotyledonary tissue. Compact in appearance, they were constituted of thin packed fibrillar structures, limited by a denser peripheral layer. It is difficult to know whether or not they had a limiting membrane. Some possessed thin dense crystals or globoids (aldehydic fixations). During this early phase, several types of organelles seemed to contribute to the development of protein bodies, including saccules of rough endoplasmic reticulum and dictyosomes. Polyribosomes constituted a network around their surface. They did not exhibit any acid phosphatase activity. In this respect, they differed from the vacuoles, saccules of endoplasmic reticulum, and several neighbouring vesicles.

Seasonally dependent formation of protein-storage vacuoles in the inner bark tissues of Salix microstachya

1990 ◽  
Vol 68 (8) ◽  
pp. 1747-1755 ◽  
Author(s):  
John S. Greenwood ◽  
Cobi Demmers ◽  
Suzanne Wetzel
Keyword(s):  
Endoplasmic Reticulum ◽  
Rough Endoplasmic Reticulum ◽  
Protein Body ◽  
Protein Bodies ◽  
Electron Microscopic ◽  
Protein Storage ◽  
Inner Bark ◽  
Protein Storage Vacuoles ◽  
Body Development ◽  
Microscopic Studies

The inner bark tissues of temperate hardwoods often act in the temporary storage of reduced nitrogen as protein during the overwintering period. Electron microscopic studies reported here demonstrate the analogy between the protein-storage vacuoles of the inner bark tissues and protein bodies in seeds. Development of these organelles parallels that of protein body formation seen in many dicotyledonous seeds. Coincident with the synthesis and sequestering of specific proteins, the large central vacuoles of the phloem parenchyma cells are slowly replaced over a 3- to 4-week period with numerous smaller protein-storage vacuoles (protein bodies). These arise via the subdivision of the larger vacuole and subsequent filling of the smaller vacuoles with protein. During this process there is a proliferation of both free ribosomes and rough endoplasmic reticulum in the ground cytoplasm. Stacks of rough endoplasmic reticulum are present in the peripheral cytoplasm and surround the smaller vacuoles as proteinaceous material is deposited. Golgi complexes, although not numerous, are present in the ground cytoplasm during the filling of the protein storage vacuoles. Key words: protein-storage vacuoles, protein body development, Salix microstachya, hardening, nitrogen storage, dormancy onset.

Immunocytochemical analysis of protein body formation in seeds of Sorghum bicolor

1989 ◽  
Vol 67 (10) ◽  
pp. 2850-2856 ◽  
Author(s):  
Hari B. Krishnan ◽  
Jerry A. White ◽  
Steven G. Pueppke
Keyword(s):  
Endoplasmic Reticulum ◽  
Sorghum Bicolor ◽  
Rough Endoplasmic Reticulum ◽  
Protein A ◽  
Sodium Dodecyl ◽  
Protein Body ◽  
Electrophoretic Analysis ◽  
Protein Bodies ◽  
Aleurone Layer ◽  
Dark Staining

Electrophoretic analysis of sorghum (Sorghum bicolor (L.) Moench) seed prolamines in the presence of sodium dodecyl sulfate reveals major proteins of 27 and 25 kDa and two other proteins of 18 and 12 kDa. Antibodies were raised against this prolamine fraction and used to examine the subcellular distribution of the proteins in developing sorghum seeds. Protein bodies in the starchy endosperm and subaleurone cells usually are round in cross section and contain darkly staining materials arranged in concentric rings. Protein bodies in the first two layers beneath the aleurone layer are irregular in shape and contain discrete pockets of light and dark staining inclusions. Prolamines were detected in both types of protein bodies by immunolabeling. Other oganelles, including Golgi complexes, mitochondria, and amyloplasts, were not labeled. The protein bodies, which have ribosomes attached to their surfaces, are directly connected to the rough endoplasmic reticulum. In some instances, this endoplasmic reticulum was specifically labeled with protein A – gold particles. Based on these observations, we suggest that the rough endoplasmic reticulum serves as the site of both synthesis and accumulation of sorghum prolamines.

Modification of Pineal Ultrastructure by Exogenous Hormones

1967 ◽  
Vol 25 ◽  
pp. 170-171
Author(s):  
R. A. Turner ◽  
A. E. Rodin ◽  
D. K. Roberts
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Rough Endoplasmic Reticulum ◽  
Estradiol Benzoate ◽  
Female Rats ◽  
List Type ◽  
Type Number ◽  
Pregnant Rats ◽  
Gonadal Atrophy ◽  
Pineal Ultrastructure

There have been many reports which establish a relationship between the pineal and sexual structures, including gonadal hypertrophy after pinealectomy, and gonadal atrophy after injection of pineal homogenates or of melatonin. In order to further delineate this relationship the pineals from 5 groups of female rats were studied by electron microscopy:ControlsPregnant ratsAfter 4 weekly injections of 0.1 mg. estradiol benzoate.After 8 daily injections of 150 mcgm. melatonin (pineal hormone).After 8 daily injections of 3 mg. serotonin (melatonin precursor).No ultrastructural differences were evident between the control, and the pregnancy and melatonin groups. However, the estradiol injected animals exhibited a marked increase in the amount and size of rough endoplasmic reticulum within the pineal cells.

Membrane-associated microtubular areays induced in proximal myelinated processes of dorsal root ganglia by large doses of vitamin B6

1986 ◽  
Vol 44 ◽  
pp. 368-369
Author(s):  
V.J. Montpetit ◽  
S. Dancea ◽  
L. Tryphonas ◽  
D.F. Clapin
Keyword(s):  
Animal Model ◽  
Endoplasmic Reticulum ◽  
Dorsal Root Ganglia ◽  
Rough Endoplasmic Reticulum ◽  
Dorsal Root ◽  
Vitamin B6 ◽  
Morphological Changes ◽  
Model System ◽  
Sensory Nerves ◽  
Peripheral Sensory

Very large doses of pyridoxine (vitamin B6) are neurotoxic in humans, selectively affecting the peripheral sensory nerves. We have undertaken a study of the morphological and biochemical aspects of pyridoxine neurotoxicity in an animal model system. Early morphological changes in dorsal root ganglia (DRG) associated with pyridoxine megadoses include proliferation of neurofilaments, ribosomes, rough endoplasmic reticulum, and Golgi complexes. We present in this report evidence of the formation of unique aggregates of microtubules and membranes in the proximal processes of DRG which are induced by high levels of pyridoxine.

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